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New application of ginsenoside Rf

A technology of ginsenoside and effective dose, applied in the field of medicine, can solve the problems such as the effect of ginsenoside Rf that is not reported in the literature, and achieve the effects of prolonging the latent period of paw withdrawal and reducing the degree of pain sensitivity.

Inactive Publication Date: 2015-03-04
BEIJING NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no literature reporting the effect of ginsenoside Rf on analgesia

Method used

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  • New application of ginsenoside Rf
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  • New application of ginsenoside Rf

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 10

[0038] Example 1 Study on the Influence of Panax notoginseng on TRPV1

[0039] 1. Experimental materials, reagents and instruments

[0040] 1.1 Experimental materials

[0041] Human embryonic kidney cells (HEK293) were purchased from Shanghai Shengbo Biomedical Technology Co., Ltd.

[0042] Escherichia coli competent BL21 was purchased from Beijing Lanyi Chemical Products Co., Ltd.

[0043] Vector plasmid pcDNA3.1 was purchased from Invitrogen Company.

[0044] The mTRPV1 plasmid was purchased from Beijing Nuolanxin Biochemical Technology Co., Ltd.

[0045] 1.2 Experimental reagents

[0046] Reagent name

company

Lipofectamine 2000 Transfection Reagent

Invitrogen

Cell culture medium (Opti-MEM, DMEM-H, EBSS)

Beijing Maojian Lianxing Technology Co., Ltd.

Tryptone

Beijing Chuangxin Huiyu Technology Co., Ltd.

Yeast Extract

Beijing Chuangxin Huiyu Technology Co., Ltd.

Sodium chloride (NaCl)

Beijing Chuangxin Hu...

Embodiment 2

[0142] Example 2 Study on the Effect of Ginsenoside Rf on TRPV1 in DRG

[0143] 1. Experimental materials, reagents and instruments

[0144] 1.1 Experimental animals

[0145] SPF grade male SD rats weighing 80-100 g were provided by Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., license number: SCXK (Beijing) 2012-0001, SCXK (Beijing) 2012-0001. Raised in SPF grade animal room, free to drink water, fed common sterile mouse food, indoor temperature was controlled at 23±2°C, and humidity was controlled at 45%-60%.

[0146] 1.2 Experimental reagents

[0147] name

company

Cell Basal Medium (EBSS)

Sigma-Aldrich

collagenase

Sigma-Aldrich

Western blot related reagents

Beijing Saichi Biotechnology Co., Ltd.

β-actin antibody

SANTA CRUZ

Horseradish enzyme-labeled goat anti-mouse

Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.

Horseradish enzyme-labeled donkey anti-goat

Beijing Zhongshan ...

Embodiment 3

[0178] The analgesic effect research of embodiment 3 ginsenoside Rf

[0179] 1. Experimental materials, reagents and instruments

[0180] 1.1 Experimental animals

[0181] SPF grade male SD rats weighing 190-210 g were provided by Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., license number: SCXK (Beijing) 2009-0007. Raised in SPF grade animal room, free to drink water, fed common sterile mouse food, indoor temperature was controlled at 23±2°C, and humidity was controlled at 45%-60%.

[0182] 1.2 Experimental reagents

[0183] Capsaicin: 0.05% dissolved in physiological saline containing 0.5% Tween20; others see Example 1 for details. 1.3 Experimental Instruments

[0184] name

company

1mL syringe

Beijing Chuangxin Huiyu Technology Co., Ltd.

Foot Tester

Italy

[0185] 2. Experimental method

[0186] 2.1 Grouping and administration of experimental animals

[0187] Control group: SD rats, male, 8 rats, first give...

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Abstract

The invention relates to the field of medicine, specifically relates to the new application of ginsenoside Rf, and in particular relates to the application of the ginsenoside Rf serving as a TRPV1 antagonist. The antagonist ginsenoside Rf is capable of remarkably inhibiting TRPV1 protein expression at the cellular level, and at the animal level, is capable of remarkably prolonging the paw withdrawal latency of a rat induced by capsaicin (CAP) and reducing the degree of sensitivity of the rat to pain. As a result, the ginsenoside Rf can be used as the TRPV1 antagonist to ease pain with TRPV1 as the target spot, and also can be used for preparing analgesics for treating diseases related to a TRPV1 receptor.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the new application of ginsenoside Rf, especially its application as a TRPV1 antagonist. Background technique [0002] In 1994, the International Association for the Study Pain (IASP) defined pain as "an unpleasant subjective sensory and emotional experience related to tissue damage or potential damage", which is clinically related to diabetes, joint Inflammation, cancer, and various pain processes after surgery. Pain medicine has gradually become the most active field in the medical field. According to a group of data released by China Pain Relief Week (October 11-17) in 2005, at least 100 million patients in my country are suffering from pain. Pain can not only seriously affect people's physical and mental health, but also It causes a great financial burden on the patient's family. According to the survey, the amount of lost productive time due to pain in the UK is 4.6 hours per week;...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61P25/00A61P29/00A61P13/12
CPCA61K31/704
Inventor 张文生刘晓丽王胜兰辛文锋
Owner BEIJING NORMAL UNIVERSITY
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