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Serratia marcescens HGS-487 strain for converting ginsenoside Rf into ginsenoside Rg1 and application

A technology of HGS-487 and ginsenoside, which is applied in the field of Serratia strains, can solve the problems of easy destruction of aglycon structure, many by-products, and low conversion rate, and achieve a clear target product, high conversion rate, and low cost. Effect

Active Publication Date: 2021-09-17
SHANXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical and chemical methods are easy to destroy the aglycon structure during the conversion process, and the conversion rate is low, the reaction is violent, and there are many by-products

Method used

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  • Serratia marcescens HGS-487 strain for converting ginsenoside Rf into ginsenoside Rg1 and application
  • Serratia marcescens HGS-487 strain for converting ginsenoside Rf into ginsenoside Rg1 and application
  • Serratia marcescens HGS-487 strain for converting ginsenoside Rf into ginsenoside Rg1 and application

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Embodiment Construction

[0013] The present invention will be described in detail below in conjunction with specific examples, but the protection scope of the present invention is not limited to the following examples.

[0014] Isolation and cultivation of strains

[0015] In this example, 0.5 g root tissue of 5-year-old fresh wild ginseng collected from Changbai Mountain was sterilized with 75% ethanol for 20 minutes, washed with sterile water for 3-5 times, put into a previously sterilized mortar, and added 5 mL Grind it into a suspension with sterile water, spread it evenly on the LA medium plate, and incubate it upside down in a constant temperature incubator at 37°C for 3 to 5 days. Use a toothpick to pick the bacteria grown on the plate to a new LA plate and repeat Purify until a single colony is obtained.

[0016] Pick the monoclonal bacteria into a 1.5mL EP tube filled with 600μL LB liquid medium, culture it on a shaker at 37°C and 190r / min for 1d, add an equal volume of 50% glycerol, mix wel...

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Abstract

The invention provides a serratia marcescens HGS-487 strain for converting ginsenoside Rf into ginsenoside Rg1. The strain is preserved in China Center for Type Culture Collection in Wuhan University, Wuhan, China; the postcode is 430072, the preservation date is December 17, 2019, and the preservation number is CCTCC NO: M20191057; and the 16SrDNA sequence of the strain is as shown in SEQ ID NO. 1. The serratia marcescens HGS-487 strain can be used for preparing ginsenoside Rg1 through fermentation and conversion.

Description

technical field [0001] The invention relates to a Serratia strain, which can transform ginsenoside Rf into ginsenoside Rg1, and belongs to the technical field of microorganisms. Background technique [0002] Ginseng (Panax ginseng) is a traditional Chinese medicinal material in my country. Ginsenoside (Ginsenoside) is the main active medicinal ingredient of ginseng, which has many physiological and pharmacological activities such as anti-tumor, anti-oxidation, protection of cardiovascular system and nervous system, and is widely used in the development of drugs and health products. [0003] Ginsenoside Rg1 has a good effect on protecting the central nervous system, can delay and reduce the apoptosis of neurons, has the effect of promoting the proliferation of cortical neural stem cells and the directional differentiation of glial-like cells, and can also reduce The content of β-amyloid protein in the brain has a good anti-Alzheimer's effect. Rg1 also has a good role in pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P33/20C12R1/425
CPCC12P33/20
Inventor 苗莉云张鹏李浩东
Owner SHANXI UNIV OF CHINESE MEDICINE
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