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Method for preparing 20-glucose-ginsenoside Rf monomers from panax japonicas

A technology of ginsenoside and glucose, applied in the field of natural medicinal chemistry, can solve the problems of many organic solvents, cumbersome process, and low purity of monomers, and achieve the effect of reducing dosage and simple process

Inactive Publication Date: 2016-02-24
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 20-glucose-ginsenoside Rf can be separated from Ginseng by column chromatography, but the process is cumbersome, more organic solvents are used, and the purity of the obtained monomer is low, and the yield is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Pearl ginseng 10 kg, 70% ethanol water reflux extraction 3 times, the amount of ethanol was 10, 8, 8 times (V / V) respectively, the reflux time was 1.5h, 1.0h, 1.0h respectively, the three extractions were combined, and the solvent was recovered , After drying, 1400 g of the extract of ginseng was obtained.

[0020] Get pearl ginseng extract 50g, separate with silica gel column chromatography, mobile phase is dichloromethane:methanol:water (6:1:0.5, volume ratio), obtain the component B10g that contains 20-glucose-ginsenoside Rf; Component B is separated by ODS column chromatography, and the mobile phase is methanol-water (1:2, volume ratio) to obtain component I8g containing 20-glucose-ginsenoside Rf; component I is separated by high-speed countercurrent chromatography (HSCCC) For separation, the solvent ratio is n-butanol-ethyl acetate-water (4:1:5, volume ratio) as the solvent system for preparative countercurrent chromatographic separation, and the flow rate is 2mL m...

Embodiment 2

[0033] Get the total extract 50g of Radix Ginseng, separate with silica gel column chromatography, mobile phase is ethyl acetate: methanol: water (7:1:0.5, volume ratio), obtain the component B9 that contains 20-glucose-ginsenoside Rf. 1g; Component B is separated by ODS column chromatography, and the mobile phase is methanol-water (1:1.5, volume ratio) to obtain 7.3g of component I containing 20-glucose-ginsenoside Rf; Countercurrent chromatography (HSCCC) was used for separation, and the solvent ratio was n-butanol-ethyl acetate-water (4:1:5, volume ratio) as the solvent system for preparative countercurrent chromatography separation, and the flow rate was 2mL min -1 , the speed is 800r·min -1 , the detection wavelength is 203nm.

[0034] The specific operation is as follows: take 1200mL of n-butanol, 300mL of ethyl acetate, and 1500mL of water, put them in a 2000mL separatory funnel, oscillate and shake well, and let stand overnight. The upper phase is the stationary phas...

Embodiment 3

[0038] Get the total extract 50g of Radix Ginseng, separate with silica gel column chromatography, mobile phase is ethyl acetate: ethanol: water (7:1:1, volume ratio), obtain the component B8 that contains 20-glucose-ginsenoside Rf. 5g; Component B is separated by ODS column chromatography, mobile phase is methanol-water (1:1.5, volume ratio), obtains the component I6.9g that contains 20-glucose-ginsenoside Rf; Countercurrent chromatography (HSCCC) was used for separation, and the solvent ratio was n-butanol-ethyl acetate-water (4:1:5, volume ratio) as the solvent system for preparative countercurrent chromatography separation, and the flow rate was 2mL min -1 , the speed is 800r·min -1 , the detection wavelength is 203nm.

[0039] The specific operation is as follows: take 1200mL of n-butanol, 300mL of ethyl acetate, and 1500mL of water, put them in a 2000mL separatory funnel, oscillate and shake well, and let stand overnight. The upper phase is the stationary phase, and th...

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PUM

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Abstract

The invention discloses a method for preparing 20-glucose-ginsenoside Rf monomers from panax japonicas. Components containing 20-glucose-ginsenoside Rf monomers are prepared, 20-glucose-ginsenoside Rf monomers are separated from components containing 20-glucose-ginsenoside Rf monomers by utilization of a high-speed countercurrent chromatography, wherein the high-speed countercurrent chromatography condition is as follows: n-butanol-ethyl acetate-water with a volume ratio of 4-6:1:5-7 is employed as a solvent system for high-speed countercurrent chromatography separation. In separation of 20-glucose-ginsenoside Rf column chromatography combined with high-speed countercurrent chromatography (HSCCC), the process is simple, the organic reagent dosage is reduced greatly, and the purity of the obtained monomers is more than 95%. The provided method for separation of 20-glucose-ginsenoside Rf monomers employs a high-speed countercurrent chromatography (HSCCC) for the first time. The obtained 20-glucose-ginsenoside Rf monomers can be used for preparation of medicine compositions, health care food and other products.

Description

technical field [0001] The invention relates to a method for preparing 20-glucose-ginsenoside Rf monomer from pearl ginseng, belonging to the field of natural medicinal chemistry. Background technique [0002] Bead ginseng is Araliaceae plant PanaxjaponicusC.A.Mey.var.major(Burk.)C.Y.WuetK.M.Feng or PanaxjaponicusC.A.Mey.var.bipinnatifidus(Seem.)C.Y.WuetK.M .Dried rhizome of Feng. Excavated in autumn, removed the rough bark and fibrous roots, dried; or steamed (boiled) thoroughly and then dried. It has the functions of nourishing the lungs and nourishing yin, dispelling blood stasis, relieving pain, and stopping bleeding; it is used for deficiency of both qi and yin, dysphoria and thirst, fatigue cough, injury from falling, arthralgia, hemoptysis, hematemesis, epistaxis, metrorrhagia, trauma bleeding. [0003] So far people have isolated a variety of saponins from Pearl Ginseng, which mainly include ginsenoside Ro, bamboo ginsenoside IVa, bamboo ginsenoside V, ginsenoside...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00
CPCC07J17/005
Inventor 李绪文李敏金永日
Owner JILIN UNIV
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