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Rahnella HGS-393 strain for converting ginsenoside Rf into Rh1 and application

A technology of HGS-393 and ginsenosides, applied in the field of Lahenia strains, can solve the problems of easily destroying aglycon structure, low conversion rate, many by-products, etc., and achieve a clear target product, high conversion rate and low cost. Effect

Active Publication Date: 2021-09-17
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Physical and chemical methods are easy to destroy the aglycon structure during the conversion process, and the conversion rate is low, the reaction is violent, and there are many by-products

Method used

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  • Rahnella HGS-393 strain for converting ginsenoside Rf into Rh1 and application
  • Rahnella HGS-393 strain for converting ginsenoside Rf into Rh1 and application
  • Rahnella HGS-393 strain for converting ginsenoside Rf into Rh1 and application

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Embodiment Construction

[0014] The present invention will be described in detail below in conjunction with specific examples, but the protection scope of the present invention is not limited to the following examples.

[0015] Isolation and cultivation of strains

[0016] In this example, 0.5 g root tissue of 5-year-old fresh wild ginseng collected from Changbai Mountain was sterilized with 75% ethanol for 20 minutes, washed with sterile water for 3-5 times, put into a previously sterilized mortar, and added 5 mL Grind it into a suspension with sterile water, spread it evenly on the LA medium plate, and incubate it upside down in a constant temperature incubator at 37°C for 3 to 5 days. Use a toothpick to pick the bacteria grown on the plate to a new LA plate and repeat Purify until a single colony is obtained.

[0017] Pick the monoclonal bacteria into a 1.5mL EP tube filled with 600μL LB liquid medium, culture it on a shaker at 37°C and 190r / min for 1d, add an equal volume of 50% glycerol, mix wel...

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Abstract

The invention provides a rahnella HGS-393 strain for converting prototype ginsenoside Rf into rare ginsenoside Rh1. The strain is preserved in China Center for Type Culture Collection in Wuhan University, Wuhan, China; the postal code is 430072, the preservation date is May 7, 2021, and the preservation number is CCTCC NO: M2021498. The 16S rDNA sequence of the strain is as shown in SEQ ID NO. 1. The rahnella HGS-393 strain can be used for preparing the rare ginsenoside Rh1 through fermentation and conversion.

Description

technical field [0001] The invention relates to a Lahnella bacterial strain, which can transform ginsenoside Rf into ginsenoside Rh1, and belongs to the technical field of microorganisms. Background technique [0002] Ginseng (Panax ginseng) is a traditional Chinese medicinal material in my country. Ginsenoside (Ginsenoside) is the main active medicinal ingredient of ginseng, which has many physiological and pharmacological activities such as anti-tumor, anti-oxidation, and protection of cardiovascular system and nervous system, and is widely used in the development of drugs and health products. [0003] Ginsenosides are divided into prototype ginsenosides and rare ginsenosides. Prototype ginsenosides refer to the rich ginsenosides directly extracted from ginseng, including Rf, Ra, Rb1, etc. Rare ginsenosides refer to ginsenoside components with stronger biological activity formed after the metabolic transformation of the prototype ginsenosides, which have very low or no c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P33/20C12R1/01
CPCC12P33/20
Inventor 张鹏田佳琳许棵李春艳黄琪雅郑娟王杨洋林兰瑾李浩东
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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