Method for determining contents of multi-index components in Weifuchun tablets

Abstract

The invention discloses a method for determining the contents of multi-index components in Weifuchun tablets, which comprises the following steps: step 1, preparing a mixed reference substance solution: precisely weighing reference substances of hesperidin, neohesperidin, rosmarinic acid, ginsenoside Re, ginsenoside Rg1, ginsenoside Rf and ginsenoside Rb1, and dissolving the reference substances in methanol to prepare the mixed reference substance solution; step 2, carrying out preparation of a test solution: taking a Weifuchun tablet sample, performing grinding, precisely performing weighing, and adding the product into a methanol solution to prepare a test solution; and step 3, carrying out determination: respectively taking the mixed reference substance solution obtained in the step 1 and the test solution obtained in the step 2, performing quantitative sample introduction, and performing determination by adopting a UPLC-QDa coupling technology. The UPLC-QDa multi-index component content determination method established by the invention is used for determining 14 batches of Weifuchun tablets sold in the market. The result shows that the method can be used for evaluating the consistency between batches of the Weifuchun tablets and the controllability of the process, the quality of the Weifuchun tablets can be comprehensively controlled, and a reference is provided for improving the quality of the Weifuchun tablets.

Description

technical field [0001] The invention relates to the technical field of content determination of traditional Chinese medicine components, in particular to a method for determining the content of multi-index components in Weifuchun Tablets. Background technique [0002] Weifuchun Tablets are recorded in the first part of the "Chinese Pharmacopoeia" 2020 edition. It is composed of three traditional Chinese medicines: red ginseng, fragrant tea, and bran-fried Fructus Aurantii. It is a national protected variety of traditional Chinese medicine. Modern medical research believes that it has the functions of suppressing cancer, sterilizing bacteria, accelerating gastric emptying, promoting gastric secretion and analgesia. After TCM syndrome differentiation, the drug is widely used clinically in chronic non-atrophic gastritis, chronic atrophic gastritis, intestinal gland metaplasia, intraepithelial neoplasia and other gastric cancer precancerous lesions, which can effectively inhibit...

AI Technical Summary

Problems solved by technology

Due to the high price, high maintenance cost, and low popularity of LC-MS / MS instruments, it cannot meet the daily sample inspection needs of traditional Chinese medicine companies, and the practical applicability of the method is not high.

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