46results about How to "Thrombolytic effect is good" patented technology

The invention discloses a thrombolysis tube, relating to the medical equipment technology field. The thrombolysis tube comprises a tube main body and a connector connected to the rear end of the tube body; the tube main body comprises an outer tube and an inner tube; the outer tube sleeves the inner tube; a drug filling cavity is a gap between the outer tube and the inner tube; a tube cavity of the inner tube is a drainage cavity; the drug filling cavity is isolated from the drainage cavity; the connector comprises a first port and a second port; the first port is communicated with the drainage cavity; the second port is communicated with the drug filling cavity; an opening is formed in the front end of the drainage cavity; the front end of the outer tube is welded together with the outer circumference surface of the inner tube so as to make the front end of the drug filling cavity sealed; a drainage hole which is used for guiding blood to the drainage cavity and a plurality of drug outlet holes communicating with the drug filling cavity are formed on the tube wall of the outer tube; the drainage hole is arranged behind the drug outlet holes; the drainage hole is communicated with the drainage cavity through the flow guiding tube; the flow guiding tube passes through the drug filling cavity and the two ends of the flow guiding tube are correspondingly connected to the drainage hole and the drainage cavity. The thrombolysis tube makes sure blood circulation is smooth and guarantees a good thrombolysis effect.

Production of natto kinase and its use are disclosed. The process is carried out by taking sugar, soy-bean and starch as raw materials, using hay bacillus high-yield thrombolytics kinase strain, deep liquid fermenting, adjusting metabolism by complex system, membrane separating, and freeze-drying to obtain the final product. It belongs to serine proteinase and can be used to melt thrombus, inhibit platelet coagulation, prevent hypertension and arteriosclerosis, adjust blood fat and keep blood flow unblocked, and it can be developed as infusion granule, tablet, capsule and oral liquid.

The invention discloses a thrombolytic liquid preparation capable of avoiding fibrinogen degradation. The thrombolytic liquid preparation is characterized in that the thrombolytic liquid preparation is prepared from FGFCI powder, a cosolvent and normal saline, wherein the concentration of the FGFCI in the thrombolytic liquid preparation is 1-5 mg/mL. The thrombolytic liquid preparation provided by the invention has advantages, in a concentration range in which thromboclasis can be performed, comprising good repeatability and quality stabilization. In addition, in-vitro Caco-2 experiments show that a proper administration route for the FGFCI is intravenous injection which fully meets the needs of clinical medication, and reduction of bleeding risk and achievement of good thrombolytic effects.

The invention discloses a preparation method of targeted thrombolytic microcapsules, which belongs to the technical field of biological materials, the method and comprises the following steps: 1) adding soluble microspheres serving as inner cores into a dopamine solution to prepare polydopamine modified microspheres; 2) further reacting the polydopamine modified microsphere with platelet ligand protein to obtain the active biological protein modified microspheres; and 3) dissolving and etching the microsphere core of the prepared microsphere through a proper mild solvent to obtain the hollow microcapsule structure capable of loading a drug, and then loading thrombolytic drugs to obtain the thrombolytic microspheres with the targeting function. Through surface modification and solvent etching technologies, the drug-loaded hollow microcapsule with good biocompatibility is prepared, and meanwhile, the preparation method and process are simple, the surface structure is stable, and excellent thrombolysis performance, blood compatibility and practicability are achieved.

This invention belongs to the biology project pharmacy filed, which provides a Staphylokinase (SAK) with low pyrogen and it's preparing method. The Staphylokinase has low pyrogen, high purify and strong activity, which is completely fit for the medicinal demand. This invention includes the following steps: a breaking up the material containing with the Staphylokinase and distilling the floating liquid; b. sieving the product from step a with the DEAD gelatin rob, then removing salt and condensing; c. sieving the product form step b with CM gelatin rob, collecting the sample apex, condensing the washing liquid and transforming the medium; d. sieving the product from step c with the Q gelatin rob to obtain the Staphylokinase. The Staphylokinase of this invention has good bolt dissolving effect, and it is safe and efficient for users..

The invention provides a fermenting production method of low-temperature douche fibrinolytic enzyme by marine microorganisms. The method comprises the following steps: directionally domesticating marine microorganisms which produce douche fibrinolytic enzyme to lead the douche fibrinolytic enzyme to grow excellently in a culturing condition; culturing the directionally domesticated douche fibrinolytic enzyme producing strain at a temperature between 10 and 16 DEG C for 24 to 36 hours, and gradually propagating in an inoculum size between 5 to 8 percent; inoculating into a liquid ferment culture medium in an inoculum size of 3 to 9 percent of the volume of a fermentation broth, and culturing for 60 to 96 hours at a temperature between 10 and 16 DEG C to complete fermenting production of the douche fibrinolytic enzyme by the marine microorganisms; centrifuging the fermenting broth in 4000 to 8000rpm to collect liquid which is a crude enzyme; and further concentrating, separating and purifying the crude enzyme according to different requirements and different use objects to prepare the enzyme preparations with different activities, purities and preparation forms.

The application belongs to the technical field of biology, and particularly relates to a preparation method of urokinase. The urokinase expressed by the method is high in output and easy to purify; the expressed urokinase proteins glycosylation is similar with the urokinase expressed by human body, and functions are the same. Moreover, the method is free from the limit of natural resources, and there is no limit to produce the urokinase with the same structure as the human body according to market demand; the method is less in investment, high in output, good in thrombolysis effect, and free from toxic side effects.

The invention provides a reteplase mutant and application thereof. The mutant comprises brand-new mutation sites of two amino acids in a self-shearing ring region, and substitution, deletion or addition of at least one of amino acid residues including alanine and proline is included. The mutant is named as reteplase mutant. The mutant has the abilities of remarkably resisting inhibition of an endogenous inhibitor (PAI-1) of the mutant and enhancing plasminogen activation, can be used as a thrombotic disease medicine and is used for treating various diseases including acute myocardial infarction, acute pulmonary embolism, cerebral apoplexy and venous thrombosis.

The invention provides agrocybe cylindracea plasmin and a preparation method thereof, and relates to the technical field of bioengineering. The agrocybe cylindracea plasmin is composed of a subunit Aand a subunit B, and the molecular weight of the subunit A is 31.4 kDa; the molecular weight of the subunit B is 21.2 kDa; the twelve amino acid sequences of the N-terminus of the subunit A are as shown in SEQ ID No.1; and the twelve amino acid sequences of the N-terminus of the subunit B are as shown in SEQ ID No. 2. The optimum temperature of the plasmin is 47 DEG C; the activity can be preserved at physiological pH of a human body, and different concentration of Fe<2+> have obvious inhibitory effects on the agrocybe cylindracea plasmin; and thrombolytic performance is good, safety is good in use, and production cost is low. A certain research basis for application in the preparation of thrombolytic drugs and/or functional foods is provided, and a new way for the comprehensive utilization of agrocybe cylindracea is further provided.

The invention discloses fusion protein of cytoglobin and sipunculus nudus plasmin, and further provides a preparation method for the fusion protein and related bacterial strain construction. The method comprises the following steps that bacterial strain culture and amplification are carried out, supernatant is separated, sulfuric acid precipitating is carried out, Sephadex G-25 desalting is carried out, and DEAE step-by-step elution and purification are carried out so as to obtain the fusion protein. According to the fusion protein, the optimum pH range is within 5.0-9.0 at 40 DEG C or below,the enzyme activity keeps relatively stable, and the influence of PMSF and Cu<2+> on the activity of fusion protease is relatively large. The fusion protein has the characteristic of targeted degradation of fibrinogen, and the degradation sequence is firstly an alpha chain, secondly a beta chain and finally a gamma chain. The fusion protein has a better thrombolytic effect on urokinase in a thrombus experiment.

The invention discloses a preparation method of natural Sipunculus fibrinolysin and application thereof. The natural Sipunculus fibrinolysin has a partial amino acid sequence of SEQ ID NO: 1. The natural Sipunculus fibrinolysin has a very good antithrombotic curative effect.

The present invention discloses a rosa roxburghii composition for anticoagulation and a preparation method thereof. The composition is prepared from the following components in parts by weight: 10-90 parts of roxburgh rose concentrated solution, 2-10 parts of rhizoma polygonati, 2-10 parts of radix puerariae, 2-10 parts of rhizoma dioscoreae, 2-10 parts of poria cocos, 2-10 parts of radix ginseng, 1-5 parts of earthworm peptide, 1-5 parts of Y-aminobutyric acid, 1-5 parts of xylitol, 1-5 parts of cordyceps militaris, 1-5 parts of mulberry and the balance of purified water. According to the rosa roxburghii beverage for anticoagulation prepared by the preparation method disclosed by the invention, the roxburgh rose concentrated solution has a good anticoagulant effect and can be used for making up the defects of heparin medicines; and meanwhile, the medicine can directly penetrate through gastric digestive juice to enter small intestines, the high anticoagulation effect is achieved, and the good thrombus dissolving effect is achieved.