217 results about "Thrombolysin" patented technology

The present invention relates to methods for the diagnosis and evaluation of stroke and stroke sub-type. A variety of bio-markers are disclosed for assembling a panel for such diagnosis and evaluation. Methods are disclosed for selecting markers and correlating their combined levels with a clinical outcome of interest. In various aspects: the invention provides methods for early detection and differentiation of stroke subtypes, for determining the prognosis of a patient presenting with stroke symptoms, and identifying a patient at risk for hemorrhagic transformation after thrombolyic therapy. Methods are disclosed that provide rapid, sensitive and specific assays to greatly increase the number of patients that can receive beneficial stroke treatment and therapy, and reduce the costs associated with incorrect stroke diagnosis.

The invention discloses a fungal fibrinolytic enzyme and a preparation method thereof. The fungal fibrinolytic enzyme is prepared by taking a Cordyceps militaris strain as a strain and performing liquid fermentation culture, separation and purification. The relative molecular weight of the fibrinolytic enzyme is about 21,000. The Cordyceps militaris fibrinolytic enzyme has good thrombolytic performance, is free from obvious acute toxicity, has prospects for clinical trial, development and application, and adds a new member to a rare fibrinolytic enzyme family. Particularly, a preferable fibrinolytic-enzyme Cordyceps militaris strain C.LSG-1 obtained through severe screening is rough in growth conditions, short in enzyme production cycle and capable of harvesting a large amount of Cordyceps militaris mycelium during enzyme production, and is the strain excellent in production performance. The Cordyceps militaris fibrinolytic enzyme is an extracellular enzyme which is particularly beneficial to subsequent separation and purification during preparation. The fungal fibrinolytic enzyme takes corn protein with extensive sources as a main culture medium, thereby having low cost for raw materials and providing a novel way for developing and utilizing the prior resources.

The invention discloses a fusing protein with plasminokin and anticoagulant function and DNA sequence to code the fusing protein, carrier with the DNA sequence and making method of fusing protein, which provides the application of fusing protein to prepare functional drug with peptide segment of continuous amino acid sequence as connecting structure, wherein the fusing protein connects 10-12 amino acids in the 55-66th amino acids sequence at IIIC end of hirudin with r-PA as t-PA bobbing mutant.

A Chinese medicine in the form of treating vasculitis, phlebitis, phlebothrombosis, lymphangitis, Raynaud's disease, etc is prepared from 6 Chinese-medicinal materials including pangolin scales, red sage root, dahurian angelica root, Chinese angelica root, etc through decocting four of them twice, concentrating the decoction, mixing with the powder of others, drying, pulverizing, sieving and loading in capsules.

The invention provides a thrombus thrombolysis and clearing device and a use method thereof, and relates to the technical field of a medical appliance. The invention aims at solving the problems thatan existing thrombus clearing device is difficult to clear the thrombus on the vascular wall, so that the thrombus clearing is unthorough. A thrombus suction head in the thrombus thrombolysis and clearing device provided by the invention is arranged on a fixed cutter holder and is rotationally connected with the fixed cutter holder; a transmission tube is arranged in an outer tube in a sleeved way; the end, provided with a through hole, of the transmission tube extends out of the outer tube; a liquid cavity bag is arranged on the outer tube wall of the outer tube between a baffle edge and a baffle ring; a sliding seat is arranged on the transmission tube in a sleeving way and is glidingly connected with the transmission tube; the sliding seat is positioned between the baffle edge and the baffle ring; and a thrombus breaking cutter is fixedly arranged between the fixed cutter holder and the sliding seat. According to the thrombus thrombolysis and clearing device, pressurized normal saline or thrombolysis medicine is sprayed out from the liquid cavity bag to break the thrombus; meanwhile, a driver drives the transmission tube to rotate at a high speed; the thrombus breaking cutter onthe sliding seat is driven to perform rotary cutting on the thrombus; and the tensioned thrombus breaking cutter can scrape away the thrombus on the vascular wall. The thrombus thrombolysis and clearing device and the use method provided by the invention are applied to the field of medical care.

The invention discloses a cDNA sequence and an amino acid sequence of coding lugworm fibrinolytic enzyme which are respectively shown in SEQIDNo.1 and SEQIDNo.2 in a sequence table, and belongs to the field of medical biological engineering. The cDNA sequence has the beneficial effects that according to the cDNA sequence of the lugworm fibrinolytic enzyme, the coding gene of the fibrinolytic enzyme can be cloned from digestive tract tissue of lugworm, and can be expressed in a prokaryotic expression system or a eukaryotic expression system through a gene recombination technology. The recombinant lugworm fibrinolytic enzyme has plasminogen activation activity, and can be used as a new thrombolytic medicament in genetic engineering.

The present invention relates to one kind of and its culturing and preparing process. The plasmin is prepared with N. sitophila strain No. 17 in the preservation number of CGMCC No. 1836, and through fermentation, separation and purification. It consists of two subunits of molecular weight 30000 and 15500 separately and isoelectric point of 7.9+/-0.2. The plasmin has high thrombolysis performance, no bleeding activity, no blood coagulating activity and no obvious acute toxicity, and thus possesses clinical test, development and application foreground. The plasmin of the present invention is one kind of extracellular enzyme, and is favorable to post separation and purification. Its preparation adopts wheat bran and soybean residue as main culture medium material, and thus has low material cost.

The invention relates to a preparation method of spirulina kinase-rich health-care food. The preparation method comprises the following steps: 1) adding shell-broken spirulina into water to obtain spirulina fermentation substrate and sterilizing; 2) inoculating the spirulina fermentation substrate with bacillus subtilis, and performing deep liquid ventilation and fermentation; 3) inoculating fermented liquor with saccharomycetes, and performing low-temperature standing and fermentation; and 4) performing low-temperature vacuum concentration on the standing and fermented liquor, drying and crushing, thus obtaining the spirulina kinase-rich health-care food. The invention further discloses a spirulina kinase health-care food prepared by adopting the spirulina kinase fermentation product. The spirulina kinase health-care food not only has higher spirulina kinase activity, but also the finished spirulina has small fishy smell, is good in mouthfeeling, and is easily accepted by people. Compared with the traditional spirulina tablets, the thrombolysis and thrombus preventing, blood lipid regulation and hemorrheology change characteristics can be greatly improved.

The invention discloses a fibrinolytic activated protein from cantharis, a preparation method and an application thereof. In the invention, the single-component protein with fibrinolytic activity is extracted from the cantharis, and the molecular weight of the protein is 95.5KD; and the protein has the advantages of easy purification and batch preparation, stronger fibrinolytic activity, high specific activity, no toxicity, small molecular weight and low antigen reaction, thus being an ideal plasmin preparation raw material as well as an ideal thrombolytic drug. The invention further discloses the preparation method of the fibrinolytic activated protein and the application of the same for preparing the drug for treating thrombotic disease.

The invention relates to the technical field of medical apparatuses and instruments, in particular to a universal thrombus smashing and extracting device with biomimetic jellyfish contraction and expansion functions. The device comprises a spraying mechanism used for spraying a thrombolytic drug, a thrombus smashing and extracting mechanism used for guiding thrombus out and connected with the spraying mechanism, a guiding mechanism used for pushing the spraying mechanism to go into a human body lumen and a power mechanism used for driving the guiding mechanism to move. According to the universal thrombus smashing and extracting device with the biomimetic jellyfish contraction and expansion functions, the spraying mechanism can go into the human body lumen by means of the guiding mechanism,then the thrombolytic drug is sprayed by the spraying mechanism so that thrombolysis can be conducted, the thrombus is smashed and extracted by the thrombus smashing and extracting mechanism, operation is convenient, the thrombus elimination speed is high, and the efficiency is high.

A composite medicine used also as the additive of cosmetics and health-care food for lowering blood viscosity, dissolving thrombus, and regulating blood pressure, blood sugar and blood fat is prepared through extracting to obtain L-polybasic metallothionein (L.pMT), lumbrikinase and antibacterial peptide, proportionally mixing it with the extracts of astragalus root and red sage root, thiooctanoic acid and 1,6-pectose-zinc biphosphate, and dispensing.

A vena cava filter device capable of releasing a thrombolytic drug is characterized in that a drug release head with a plurality of irregular holes is arranged in the center of a filter, the drug release head and a drug conveying pipe are connected and arranged in a filter body connecting pipe, and one end of the drug conveying pipe is provided with a drug injection hole. According to the vena cava filter device, thrombi are stopped by the vena cava filter, the thrombolytic drug is injected into the drug conveying pipe in the a filter body connecting pipe through the drug injection hole, and if the thrombi are stopped by the vena cava filter, the thrombolytic drug is directly injected to the thrombi through the irregular holes; it is not needed that venipuncture is carried out on a patient again to achieve thrombolysis, so that secondary injuries to the patient are reduced and risks brought by venipuncture to the patient are avoided; the vena cava filter device has the dual effects of stopping the thrombi and achieving thrombolysis.

The invention discloses a water gel material with thrombin responsive thrombolysis capability and a preparation method thereof, the water gel material with thrombin responsive thrombolysis capability is prepared as follows thrombin substrate peptide is used as a crosslinking agent, water gel is formed by free radical polymerization, meanwhile the water gel coats thrombolysis active molecules, and the thrombolysis active molecules only can significantly release and play fibrinolytic function. The material has the thrombin responsive thrombolysis capability, avoids the defect of a series of complications caused by the long-term exposure of reactive molecules of traditional thrombolysis materials, and has application prospect in the aspect of human implanted blood contact materials.

The invention relates to a method for preparing a thrombolytic medicament Reteplase (rPA) directly through an escherichia coli expression system without inclusion-body renaturation. A gene segment of the rPA is obtained through PCR amplification, and is cloned into a vector pET40b to construct a pET40b-rPA recombinant plasmid, and the rPA is made to perform fusion expression with the protein disulfide isomerase DsbC in the vector pET40b. The recombinant plasmid is transformed into the escherichia coli BL21 (DE3), and induction expression conditions of IPTG and lactose are respectively established optimally. The obtained target fusion protein is mainly expressed by soluble components, and is purified by Ni-NTA affinity chromatography and processed by a Xa factor (or formic acid or thrombin) to form the high-purity rPA target protein. Fibrin plate method detection results show that the fusion protein DsbC-rPA obtained by the method and the rPA protein obtained by removing a label and purifying have obvious thrombolytic activity.

The present invention relates to a compound simultaneously having triple activities of thrombolysis, antithrombosis and free radical scavenging, as well as the preparation method, composition, and applications thereof. The compound is represented by the formula I shown below:

wherein the definitions of T, Q, R₁ and R₂ are described herein. The compound of the present invention simultaneously has triple functions of thrombolysis, free radical scavenging and thrombus-targeting/antithrombosis. The present invention also relates to a pharmaceutical composition comprising the compound, and a preparation method and a nanostructure of the compound.

The invention provides a determination method of fingerprint of Poecilobdella manillensis. Poecilobdella manillensis, commonly known as yellow-stripped leech, belongs to Arhynchobdellida, Hirudinidae and Poecilobdella, has evident activities on anticoagulation, antithrombosis, thrombolysis and platelet aggregation resistance, and has evident medicinal value. Up to now, no pharmacopeia or local standard records Poecilobdella manillensis, and no reports on fingerprint of Poecilobdella manillensis are present. Poecilobdella manillensis material is determined by efficient capillary electrophoresis, a fingerprint of the Poecilobdella manillensis material is established, and a method of overall quality estimation on the Poecilobdella manillensis material is provided and is used to control the quality of the Poecilobdella manillensis material.

The invention discloses cicada slough protease enzymolytic products and an application thereof. The cicada slough protease enzymolytic products are obtained by the following steps particularly: A, selecting cicada slough, crushing into fine powder; B, putting the cicada slough fine powder in artificial gastric juice, adding pepsase for hydrolysis to obtain a pepsase enzymolytic liquid, then performing centrifugation to obtain an supernatant and residues to keep in reserve; C, taking the supernatant, drying to obtain a cicada slough pepsase enzymolytic product; D, taking the residues, transferring the residues into artificial intestinal juice, adding trypsin for hydrolysis to obtain a cicada slough trypsin enzymolytic product. The pepsase enzymolytic product obtained in step C and the trypsin enzymolytic product obtained in step D are the cicada slough protease enzymolytic products. The cicada slough protease enzymolytic products have anticoagulation or thrombolytic effect.

A catheter-implemented transducer device for intravascular thrombolysis, is described herein. Such a transducer device includes a catheter defining a longitudinal axis and having opposed proximal and distal ends. At least one ultrasonic transducer arrangement is disposed about the distal end. The ultrasonic transducer arrangement is oriented with acoustic waves propagating parallel or perpendicular to the longitudinal axis. Optionally, the ultrasonic transducer arrangement is configured as a multi-layer stacked structure of ultrasonic transducer elements. Optionally, the ultrasonic transducer arrangement is a laser ultrasonic transducer arrangement. Optionally, the ultrasonic transducer arrangement is configured to operate in a lateral mode.

The invention discloses four (R)-6, 7-dihydroxy-1, 1-dimethyl isochroman-3-formyl-AA (AA is L-Phe, L-His, L-Trp and L-Tyr residues) of the following formula, a preparation method thereof and an application of the (R)-6, 7-dihydroxy-1, 1-dimethyl isochroman-3-formyl-AA in thrombolytic drug preparation. Therefore, the invention discloses an application of the compounds in preparation of thrombolyticdrugs.

The invention discloses a recombined bifunctional plasmin freeze-drying preparation for injection and a preparation method thereof. The recombined bifunctional plasmin freeze-drying preparation is prepared from a recombined bifunctional plasmin stock solution, mannite, a Tris buffer solution, tween 80, zinc chloride and sterile injection water. The preparation method comprises the following steps of: respectively preparing the mannite, the Tris buffer solution, the tween 80 and the zinc chloride into diluents by using the sterile injection water, and then regulating the recombined bifunctional plasmin stock solution until the concentration is 100 mg/ml; mixing in proportion, then filtering by using a microporous filtering film, and charging into bottles in a splitting manner; and freeze-drying to form the finished preparation in the shape of a powder block. The finished preparation is preserved under the condition of 2-8 DEG C, and the preservation period of the active components of the finished preparation can reach 30 months. The invention has stable property and double functions of thrombolysis and platelet aggregation inhibition, can be preserved for a long time by being accelerated at 55 DEG C for 3 months and accelerated at 37 DEG C for 6 months and at room temperature, keeps the plasmin content and the solubility basically unchanged without obvious degradation and keeps the activity more than 80 percent.