Long-noded pit viper poison dissolving fiber protein No.2 gene and use thereof
A gene and genetic engineering technology, applied in the preparation of fibrin-dissolving drugs and the field of embolism diseases, can solve the problems of low market share, inexplicability, toxic and side effects, etc., achieve huge social and economic benefits, and good thrombolytic effect , the effect of less bleeding reaction
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[0037] 1. Separation of fibrinolytic factor FII from snake venom in five steps
[0038] 1. DEAE-Sephadex A-50 ion exchange chromatography
[0039] Method: DEAE-Sephadex A-50 ion-exchange gel was rinsed repeatedly in 0.05M ammonium acetate buffer with pH 8.0, soaked at 25°C for about 24 hours, and then packed into a column (gel column diameter 2.6cm, height 100cm). When the pH value of the effluent is close to 8.0, the balance is completed. 3g of five-step snake venom crude venom is dissolved in 10ml of 0.05M ammonium acetate buffer solution with pH 8.0, centrifuged at 1500rpm for 15 minutes to remove sediment, and then put on the column. Use 750ml each of 0.05M pH 8.0 ammonium acetate buffer and 1M pH 5.0 ammonium acetate buffer for linear gradient elution, and the eluent collection rate is 4tube / h, 3ml / tube. Light absorption values were measured at a wavelength of 280 nm. The fibrinolytic activity of each peak was measured, and the components with fibrinolytic activity we...
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