Fermenting production method of low-temperature douche fibrinolytic enzyme by marine microorganisms

Abstract

The invention provides a fermenting production method of low-temperature douche fibrinolytic enzyme by marine microorganisms. The method comprises the following steps: directionally domesticating marine microorganisms which produce douche fibrinolytic enzyme to lead the douche fibrinolytic enzyme to grow excellently in a culturing condition; culturing the directionally domesticated douche fibrinolytic enzyme producing strain at a temperature between 10 and 16 DEG C for 24 to 36 hours, and gradually propagating in an inoculum size between 5 to 8 percent; inoculating into a liquid ferment culture medium in an inoculum size of 3 to 9 percent of the volume of a fermentation broth, and culturing for 60 to 96 hours at a temperature between 10 and 16 DEG C to complete fermenting production of the douche fibrinolytic enzyme by the marine microorganisms; centrifuging the fermenting broth in 4000 to 8000rpm to collect liquid which is a crude enzyme; and further concentrating, separating and purifying the crude enzyme according to different requirements and different use objects to prepare the enzyme preparations with different activities, purities and preparation forms.

Description

technical field [0001] The invention relates to the fields of microbiology, enzyme engineering, fermentation engineering, biochemistry and the like, and in particular relates to a marine microbial fermentation production method of low-temperature tempeh thrombolytic enzyme. The marine low-temperature tempeh thrombolytic enzyme produced by the method has unique advantages in dissolving thrombus and activating plasminogen in the body, and can be widely used in the production of thrombolytic drugs and thrombolytic health products. Background technique [0002] Soybean thrombolytic enzyme (Nattokinase, referred to as NK) is an alkaline serine protease, non-toxic, can be taken orally (Li Jiangwei et al., Chinese Journal of Pharmaceutical Sciences, 1999), easily absorbed by the body, can directly dissolve fibrin, and has a strong thrombolytic effect. function (Hao Zhenghong et al., Chinese Journal of Agricultural Engineering, 2008; Peng Yong et al., High-Tech Communication, 2002),...

AI Technical Summary

Problems solved by technology

The optimum action temperature of NK produced by ordinary microbial fermentation is generally 45-55°C, while the physiological temperature of the human body is generally 36.5-37°C, so that the NK produced by ordinary microbial fermentation cannot fully play its role, and the therapeutic effect is poor or ineffective.