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Mode creature method for medicament toxicity research

A technology of drug toxicity and application mode, which is applied in the direction of testing pharmaceutical preparations, material inspection products, etc., and can solve problems that have not yet been applied

Inactive Publication Date: 2008-07-09
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestic research on zebrafish is mainly limited to developmental genetics, and has not yet been applied to pharmacy, especially toxicology research

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Study on Toxicity of Triptolide with Zebrafish Model Organism--LD 50 Determination of value.

[0017] Test drug: triptolide;

[0018] Source: Institute of Dermatology, Peking Union Medical College, Chinese Academy of Medical Sciences (Jiangsu, Nanjing);

[0019] Physical and chemical properties: white powder; content: 94%;

[0020] Preparation method: weigh triptolide according to the required concentration and dissolve it in 100% DMSO, then dilute the triptolide DMSO solution in ultrapure water at a ratio of 0.5%.

[0021] Animal: Zebrafish, provided by the Institute of Model Biology, Nanjing University.

[0022] Reagents: Dimethyl sulfoxide (DMSO) was purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd., and ultrapure water was obtained from a Millipore ultrapure water machine.

[0023] experimental method:

[0024] Take 120 zebrafish, place them in jars filled with 50mL solution, and divide them into 8 groups randomly, each group contains 15 fish, which a...

Embodiment 2

[0029] Study on the Toxicity of Matrine with the Model Organism Zebrafish--LD 50 Determination of value.

[0030] Test drug: matrine;

[0031] Source: Jiangsu Tiansheng Pharmaceutical Co., Ltd.;

[0032] Physical and chemical properties: light yellow powder; content: 98%;

[0033] Preparation method: according to the required concentration, take matrine and dissolve it in 100% DMSO, and then dilute the matrine DMSO solution in ultrapure water at a ratio of 0.5%.

[0034] Animal: Zebrafish, provided by the Institute of Model Biology, Nanjing University.

[0035] Reagents: Dimethyl sulfoxide (DMSO) was purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd., and ultrapure water was obtained from a Millipore ultrapure water machine.

[0036] experimental method:

[0037] Take 105 zebrafish, place them in jars filled with 50mL solution, and divide them randomly into 7 groups, each containing 15 zebrafish. For the administration groups of 150.0, 127.5, 108.4, 92.1, and 78...

Embodiment 3

[0042] Study on the toxicity of emodin with the model organism zebrafish--LD 50 Determination of value.

[0043] Test drug: emodin;

[0044] Source: Ruilei Phytochemical Preparation Factory (provider of standard products of China Inspection Institute);

[0045] Physical and chemical properties: yellow powder; content: 98%;

[0046] Preparation method: Weigh emodin according to the required concentration and dissolve it in 100% DMSO, then dilute the emodin DMSO solution in ultrapure water at a ratio of 0.5%.

[0047] Animal: Zebrafish, provided by the Institute of Model Biology, Nanjing University.

[0048] Reagents: Dimethyl sulfoxide (DMSO) was purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd., and ultrapure water was obtained from a Millipore ultrapure water machine.

[0049] experimental method:

[0050] Take 105 zebrafish, place them in jars filled with 50mL solution, and divide them randomly into 7 groups, each containing 15 zebrafish. ×10 3 , 1.4×10 3 , 0...

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PUM

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Abstract

A method for studying toxicity of drugs by using model organism zebra fish comprises the following steps of: selecting 100 to 200 adult zebra fishes, respectively disposing in bottles containing a solution of 30 to 50 mL, keeping the temperature in the bottles substantially constant at 22 to 25 DEG C, randomly grouping, each group containing 10 to 20 fishes, wherein the solution in one of the groups is pure water (blank), the solutions in other groups are medicinal solutions with different concentrations, and 0.5% to 2% dimethylsulfoxide (DMSO) can be added to assist dissolving a drug that has poor water solubility, when an additional solvent comparison group prepared by adding 0.5% to 2% DMSO into the pure water is needed; recording the death number of zebra fishes in the medicinal solutions of different concentrations within 24 h, and calculating the death rate (%); calculating median lethal dose-LD50 by Bliss method according to the experiment result; and representing the acute toxicity by the value of LD50. According to the method, the LD50s of zebra fish of triptolide, matrine and emodin are respectively 5.39*10<-3>, 112.3 and 1.08*10<3> mug / mL. The method can objectively reflect the toxicity of drugs.

Description

Technical field: [0001] The invention relates to the field of drug toxicity research and relates to a method for using a model organism zebrafish to study drug toxicity. Specifically, it refers to the toxicity studies of compounds such as triptolide, matrine and emodin using the model organism zebrafish. This method can be used as a new method for rapid drug toxicity research. Background technique: [0002] Drugs are safe, effective and quality controllable, and the three are indispensable. If there is a problem with the safety of the drug, its toxicity will cause harm to the patient, and even cause serious toxic reactions. According to reports, in the past 40 years, 2.9% of new drugs launched in the United States were withdrawn from the market by the FDA due to serious adverse reactions. Since 1993, 7 new drugs were forced to withdraw from the market after more than 1,000 patients died after being launched. We know that three parts of medicine are poisonous, and if used ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/15
Inventor 张陆勇舒斌韦英杰
Owner CHINA PHARM UNIV
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