Rapid and high-flux acute toxicity test method for luminous bacteria

A technology of luminescent bacteria and testing methods, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as taking a long time to recover or pre-culture, unfavorable toxicity screening of large numbers of samples, and prolonging toxicity testing time. , to shorten the toxicity test time, achieve high-throughput quantification, improve the sensitivity and the accuracy of test data
CN102465167AInactive Publication Date: 2012-05-23RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
0 Cites 14 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
Publication Date
2012-05-23
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
Patent Text Reader

Abstract

The invention relates to a rapid and high-flux acute toxicity test method for luminous bacteria, belonging to technical fields of analysis and detection. A luminous bacteria reviving solution is developed and can be used for rapidly reviving lyophilized luminous bacteria powder and cryopreserved strains within 30min, and the revived bacterium liquid can be directly used for a toxicity test as a working bacterium liquid without pre-culture after being stirred for a certain time. Meanwhile, a microporous plate is selected to serve as an exposing container and a detection tank for the toxicity test, a sample to be tested and the working bacterium liquid are added into pores and exposed for 15min, and then can be subjected to a luminous intensity test by adopting a chemiluminescence ELISA (Enzyme-Linked Immunosorbent Assay) reader. By adoption of the method, not only is a tedious and time-consuming working bacterium liquid pre-culture procedure omitted, but also the detection time is saved, the cost is reduced, the accuracy of a test result is increased, the high flux of the toxicity test is realized, and a wide popularization and application prospect is achieved.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a rapid and high-throughput acute toxicity test method for luminescent bacteria, which belongs to the technical category of environmental detection and evaluation. Background technique

[0002] With the progress of society and the development of industrial and agricultural production, more and more chemicals are produced and used. At present, there are more than tens of millions of registered chemicals, and more than 100,000 chemicals in daily use. These chemicals may enter the environment through various channels during production and use, and then accumulate in the human body and organisms, posing a serious threat to the ecological environment and human health. In order to effectively manage chemicals and accurately evaluate the safety of environmental samples, biological toxicity testing has gradually developed into an essential and most intuitive and effective method.

[0003] Luminescence bacterial toxicity detection is a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More