40results about How to "Shorten experimental operation time" patented technology

The invention discloses a preparation method of ultrathin slices aiming at a Hainan rubber tree leaf tissue. The preparation method comprises a preparation condition, a preparation device, preparationof various reagents and preparation of the ultrathin slices. A whole flow of the preparation method of the ultrathin slices aiming at the Hainan rubber tree leaf tissue comprises the following steps:firstly, infiltrating plant leaves in an immersion solution of glutaric dialdehyde; then fixing by utilizing osmic acid and dehydrating with ethanol; infiltrating by utilizing Epon 812 and embeddingand trimming blocks to prepare the ultrathin slices; dyeing by utilizing uranium acetate and lead citrate; finally, carrying out microscope inspection. By adopting the preparation method, the problemsof a traditional preparation method of ultrathin slices of plants including the grass family and the like for Hainan rubber tree leaves that a cell tissue structure is not clear in a transmission electron microscope sample preparation process, the slices are cracked when meeting water, an embedding agent is separated from the tissue, the embedding agent is easily affected with dampness and the like are solved; the experiment operation time is effectively shortened, and an effective technical method is provided for researching an ultra-microstructure of the rubber tree leaves.

The invention provides a human HLA-B27 gene typing kit. The human HLA-B27 gene typing kit comprises a PCR amplification primer, DNA polymerase and a reaction buffer solution, wherein the DNA polymerase is a hot initiated Taq polymerase which is modified by polyethylene glycol (PEG) and can tolerate PCR reaction inhibitors in blood. The invention also provides a nucleotide sequence of the PCR amplification primer. The human HLA-B27 gene typing kit has the technical effects that the adopted DNA polymerase is the hot initiated Taq polymerase which is modified by PEG and can tolerate PCR reaction inhibitors, PCR amplification on whole blood of human (nucleic acid extraction does not need to be carried out) can be realized, PCR amplification is directly carried out by virtue of blood, and DNA extraction does not need to be carried out; the human HLA-B27 gene typing kit can realize HLA-B27 negative and positive identification on a sample; meanwhile, a gene subtype result of an HLA-B27 positive sample can be obtained, and 24 HLA-B27 gene subtypes and other gene subtypes of 13 HLAB sites can be detected by every eight holes (eight linked pipes).

The invention relates to the field of sterile detection, in particular to a fully closed membrane filter for sterile examination of a sterile packaging interlayer and a detection method of the fully closed membrane filter. The fully closed membrane filter comprises two bacterium collecting incubators and an air filter device, wherein the two bacterium collecting incubators and the air filter device are connected to one end of a three-phase circulator by virtue of a conduit; the other end of the three-phase circulator is connected to a sterile sampling needle by virtue of a detection tube; and an intercepting clip is arranged on the part, close to the three-phase circulation valve (the three-phase circulator), of the conduit. By virtue of the fully closed membrane filter disclosed by the invention, secondary pollution on leaching liquor during operating is reduced, a microorganism detection rate is improved and an experimental result is better guaranteed. The circulation of medicine liquid in a sealed space is achieved and the medicine liquid is prevented from getting into contact with external side, so that the fully closed membrane filter plays a great role in the prevention of microbial pollution. Meanwhile, as for operation time, the experimental operation time is shortened by half, the detection efficiency of test article sterile examination is greatly improved, and a detection pollution rate is reduced to be less than 5% from original 20-25%.

The invention discloses a chicken whole blood simple, convenient and quick DNA extraction method which comprises the following steps of after adding chicken whole blood in an anticoagulant, adding a mixture in lysate for mixing, putting a mixture in a water solution boiler with the temperature being 56-60 DEG C for 20-30min, adding a separating medium in a product, putting a mixture in the water solution boiler with the temperature being 70-80 DEG C for 20-30min for centrifugation, taking a supernate, transferring the supernate into another EP pipe, adding a precipitated solution, performing centrifugation, reserving precipitates, adding a washing solution, and performing uniform mixing and centrifugation; and carefully discarding the supernate, collecting the precipitates, drying the precipitates at the room temperature, and adding a TB buffer liquid to obtain a chicken DNA extracting solution. By adopting the chicken whole blood simple, convenient and quick DNA extraction method provided by the invention, the purity and the integrity of extracted DNA can both reach test requirements, and the concentration and the purity are superior to that of DNA extracted by an existing kit. Bycombining with application on a chicken molecular breeding aspect, proved that the method is an environment-friendly DNA extraction method with low cost and simple operation.

The invention relates to a method for directly converting plasma into hydrosulfite and application thereof. The method provided by the invention, in comparison with a similar hydrosulfite converted product on the market, has the greatest advantages that free DNA (Deoxyribonucleic Acid) of the plasma does not need to be extracted, the plasma can be directly used as a hydrosulfite conversion sample,and not only is the time of extracting the free DNA saved, but also the loss of the free DNA in an extraction process is avoided. Secondly, according to the method provided by the invention, in orderto protect the DNA from being influenced by miscellaneous protein, lipid and the like, a protective agent is added into a hydrosulfite conversion system. In addition, in order to further improve therecovery rate of the free DNA, the method adopts a high-sensitivity magnetic bead to carry out recovery. The entire conversion process does not exceed 2 hours; the centrifugal operation is not involved; the automation is more easily realized, and the method provided by the invention has the advantages of being low in cost, simple and convenient to operate, high in conversion ratio, high-purity inobtained DNA and the like.

The invention relates to a fluorescence in-situ hybridization method for endosymbiotic bacteria in an insect ovary. The method comprises following steps: the insect ovary is dissected under a microscope, the dissected insect ovary is transferred to a glass slide, an insect tissue stationary liquid is added for fixation, and a destaining solution is added for destaining; pre-hybridization and hybridization staining are performed on a specimen, and an eluant is added for elution after hybridization is completed; finally, a sample is sealed with glycerin, and observation and photographing are performed under a confocal microscope. By means of the method, a good cell staining effect can be kept, the operation can be simplified, time can be shortened, reagents can be saved and the cost can be reduced. Several endosymbiotic bacteria in the ovary can be subjected to multiple staining simultaneously, a good staining effect is obtained, and the method is simple, convenient, rapid and economical.

The invention relates to a method for improving the fluorescence quantum yield of II-VI family quantum dots, and belongs to the technical field of nano materials and inorganic materials, and the method comprises the following steps: adding an anion source and a reducing agent into deionized water, and stirring and dissolving in a nitrogen atmosphere to form a transparent solution, thereby obtaining an anion precursor solution; dissolving a cation source in deionized water, adding a ligand, uniformly stirring, regulating the pH value to 7.5-10.5 by using an alkaline medium, and introducing nitrogen for half an hour to obtain a cation precursor solution. A CdTe quantum dot solution is subjected to post-treatment by adopting ZnCl2, and dangling bonds on the surface of the quantum dot are passivated by utilizing Zn < 2 + >, so that the surface defect of the quantum dot is passivated under the conditions that the band gap of the CdTe quantum dot is not influenced and the original light-emitting wavelength of the quantum dot is kept, and the fluorescence quantum yield is effectively improved; and the fluorescence quantum yield of the obtained product is greater than 30%.

The invention provides a multichannel small animal ultrasonic stimulation device. The multichannel small animal ultrasonic stimulation device is characterized by comprising a supporting mechanism, a motion control mechanism, a treatment head fixing frame, a multichannel ultrasonic stimulator and a plurality of ultrasonic treatment heads, wherein the supporting mechanism is provided with an animal experiment platform and a support arranged on the animal experiment platform; the motion control mechanism is provided with a plurality of three-dimensional motion units and a plurality of rotary motion units, and the three-dimensional motion units are arranged on the support; the treatment head fixing frame is arranged on the three-dimensional motion units and used for moving in the front-back direction, the up-down direction and the left-right direction under the driving of the three-dimensional motion units; the multichannel ultrasonic stimulator is fixed to the support and used for transmitting pulse signals; and the ultrasonic treatment heads are installed on the treatment head fixing frame through the rotary motion units respectively and used for rotating under the driving of the rotary motion units, each ultrasonic treatment head is provided with an ultrasonic transducer, and the ultrasonic transducers are in communication connection with the multichannel ultrasonic stimulator and used for converting the pulse signals into ultrasonic stimulation signals. The multichannel ultrasonic stimulator is connected with the ultrasonic transducers through a plurality of cables respectively, and therefore the pulse signals are transmitted.

The invention mainly discloses a method and device for testing the function of refining of fusion metal grains through ultrasonic waves. A single-chip microcomputer, an ultrasonic pulse generation receiver, ultrasonic probes and a heating device are included. The single-chip microcomputer and the ultrasonic pulse generation receiver are connected through a wire. The ultrasonic pulse generation receiver is connected with the ultrasonic probes through a multi-direction connector. An alloy is placed on the heating device, the single-chip microcomputer receives upper computer instructions, and signals are transmitted to the ultrasonic pulse generation receiver through the single-chip microcomputer. The ultrasonic pulse generation receiver generates negative-high-voltage pulse signals to drivethe multiple ultrasonic probes to send ultrasonic waves, and the ultrasonic waves refine the alloy liquefied after heating. After the ultrasonic probes stop working and the alloy is cooled and solidified, and the alloy refined through the ultrasonic waves is cut to be observed. The single-chip microcomputer is utilized for control, the multiple ultrasonic probes are controlled, and under the sameoutside conditions, the fusion metal grain refining functions are compared under different ultrasonic frequencies and ultrasonic action time periods.

The invention provides an adherent tumor cell cryopreservation method and an improved cryopreservation solution formula. By configuring an improved cell cryopreservation solution, the intermediate centrifugation step is omitted, so that the experimental operation time is greatly shortened, the experimental consumables can be saved, the experimental efficiency is increased, and the problems that the traditional adherent tumor cell cryopreservation method is longer in experimental operation time, needs to remove the culture solution by means of centrifugation, and is cumbersome in operation process and larger in damage to the cells, the activity of the resuscitated cells is lower, and the like can be solved. The adherent tumor cell cryopreservation method and the improved cryopreservation solution formula belong to the technical field of biology.

The invention provides a method for detecting biological activity of granulocyte colony stimulating factors. The method comprises the following three steps: constructing a cell line, preparing a standard substance and a test sample, and determining. The detection method is more convenient to operate, cells do not need to be specially treated, the operation time is shortened, and the experiment operation time is shortened by 1-2 days; meanwhile, according to the method, the detection flux is improved, the sample is diluted by 6-12 concentration points, the sample can be vertically arranged in a 96-well plate, and the sample detection number of each cell plate is increased.

The invention discloses a method for testing chlorine resistance of an automobile part made of a polyamide material, and belongs to the technical field of performance testing of high polymer materials. The testing method comprises the steps that n cuboid splines are cut out from the surface of the automobile part made of the polyamide material, one end, in the length direction, of each spline is marked, the midpoint position of the tail end of each spline is marked as B, and the center, located on the same side of the midpoint of the tail end and having a certain distance from the midpoint of the tail end, of each spline is marked as A; one unmarked end of the sample strip in the step 1) is fixed on a clamping part of a clamp, the other marked end of the sample strip is positioned below a pulse pressure head, and the pulse pressure head is over against a point A of the sample strip; then, the influence of the chlorine salt on the mechanical property of the automobile part made of the polyamide material is judged by measuring the deformation quantity of the sample strip under different salt mist concentrations, different load acting forces and different times. The testing method designed by the invention not only is simple in sample preparation, but also is high in objective accuracy of an evaluation system.

The Invention discloses a preparation method of silage leach liquor, which is particularly practiced by mixing and immersing sterilized distilling water with silage; performing sonic oscillation afterlow-concentration hydrochloric acid treatment; filtering by a filter membrane, wherein the filter fluid is the silage leach liquor. Besides, the invention further provides an application of silage leach liquor prepared by the preparation method in the silage detection. Diluted hydrochloric acid with 1% of mass concentration is added during the preparation process of silage leach liquor; on the one hand, the low-concentration diluted hydrochloric acid can promote the complete dissolution of fermenting indexes in the silage such as organic acid and ammoniacal nitrogen; on the other hand, the viscosity between the silages is weakened after being treated by hydrochloric acid, the solid and fluid can be separated rapidly in the process of filtering by adopting the filter membrane, thereby greatly shortening the experimental operation time and saving labor force and lab consumables.

The invention relates to the technical field of sugar content determination, and discloses a sucrose-glucose-fructose content determination kit and a determination method thereof. The sucrose-glucose-fructose content determination kit comprises a kit body, a reagent tube, a movable connecting device, a sealing cover and a locking device, wherein the reagent tube is fixedly arranged in the kit body; the movable connecting device is fixedly arranged on the right side of the kit body; and the sealing cover is fixedly arranged at the right end of the movable connecting device. According to the sucrose-glucose-fructose content determination kit and the determination method thereof, specific enzymes respectively act on sucrose, glucose and fructose, background interference of other substances ina sample is eliminated, and the detection result is accurate; and moreover, the three pieces of sugar data can be detected at one time, the experimental operation time is shortened, personal errors caused by batch detection are reduced, and a time-saving, money-saving and nontoxic detection means is provided for detection of a large number of samples.

The invention discloses a tissue slice experimental treatment device which comprises a box body, the upper end of the box body is connected with a box cover, a plurality of slice frames capable of being placed in a stacked mode are arranged in the box body, and slice frame handles capable of being freely detached are connected to the slice frames. The invention further discloses a using method ofthe tissue slice experimental treatment device. The problem that the efficiency is low when a slice experiment is conducted at present is solved.