Preparation method of ultrathin slices aiming at Hainan rubber tree leaf tissue

Abstract

The invention discloses a preparation method of ultrathin slices aiming at a Hainan rubber tree leaf tissue. The preparation method comprises a preparation condition, a preparation device, preparationof various reagents and preparation of the ultrathin slices. A whole flow of the preparation method of the ultrathin slices aiming at the Hainan rubber tree leaf tissue comprises the following steps:firstly, infiltrating plant leaves in an immersion solution of glutaric dialdehyde; then fixing by utilizing osmic acid and dehydrating with ethanol; infiltrating by utilizing Epon 812 and embeddingand trimming blocks to prepare the ultrathin slices; dyeing by utilizing uranium acetate and lead citrate; finally, carrying out microscope inspection. By adopting the preparation method, the problemsof a traditional preparation method of ultrathin slices of plants including the grass family and the like for Hainan rubber tree leaves that a cell tissue structure is not clear in a transmission electron microscope sample preparation process, the slices are cracked when meeting water, an embedding agent is separated from the tissue, the embedding agent is easily affected with dampness and the like are solved; the experiment operation time is effectively shortened, and an effective technical method is provided for researching an ultra-microstructure of the rubber tree leaves.

Description

technical field [0001] The invention relates to the field of micromorphology of rubber tree leaf tissue, in particular to a method for preparing ultrathin slices of Hainan rubber tree leaf tissue. Background technique [0002] A transmission electron microscope (TEM) emits a high-speed electron beam through an electron gun and irradiates the sample evenly through a condenser. The incident electrons and the sample will collide and inelastically collide, forming elastic scattering electrons, loss electrons and absorption electrons. The generated light wave interferes and diffracts again, so that the uniform incident electron beam becomes uneven after interacting with the sample, and then magnified by the objective lens, intermediate mirror and projection mirror in turn to present an image on the fluorescent screen or film, reflecting the sample It is an important tool for observing and studying microstructures such as submicrostructure or ultrastructure of matter. [0003] Tr...

AI Technical Summary

Problems solved by technology

The cell wall of the rubber tree leaf tissue is thick and dense. When the traditional method is used, the penetration of the liquid medicine is often insufficient. Except for the cell wall, other structures are blurred and cannot be observed and analyzed normally.

[0005] In addition, the annual average temperature in Hainan is 22-26°C, the annual sunshine is 1750-2650 hours, the light rate is 50%-60%, the light is sufficient, the photosynthetic potential is high, and the annual humidity is ≥85%. Under the environmental conditions where the humidity cannot be precisely controlled, the slices will shrink and break due to the softening of the embedding material block, the thickness of the prepared slices will be too thick, and the cell structure will be blurred during microscopy, which will lead to the failure of sample preparation

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