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Method for preparing solid granular biochemical reagent

A technology of biochemical reagents and solid particles is applied in the field of preparation of biochemical reagents, which can solve the problems of inaccurate measurement of biochemical reagents, and achieve the effects of facilitating sub-packaging, saving operation time and increasing storage time.

Active Publication Date: 2012-12-19
CHENGDU SEAMATY TECH
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the existing biochemical reagents cannot be accurately measured under the condition of weightlessness or turbulence, and provide a method for preparing a solid granular biochemical reagent that can be accurately measured and is easy to operate

Method used

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  • Method for preparing solid granular biochemical reagent
  • Method for preparing solid granular biochemical reagent
  • Method for preparing solid granular biochemical reagent

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Experimental program
Comparison scheme
Effect test

Embodiment

[0023] A method for preparing a solid granular biochemical reagent mainly consists of the following steps:

[0024] (1) Configure concentrated biochemical reagents;

[0025] In this example, the preparation of alanine aminotransferase (ALT) solid particle biochemical reagent is taken as an example. The alanine aminotransferase is concentrated by 2.4 times, and its formula is shown in Table 1:

[0026] Table 1

[0027] tris hydroxymethyl amino methane

28.952g

L-alanine

106.462g

Alpha-ketoglutarate disodium salt, dihydrate

8.107g

EDTA.Na 2 .2H 2 o

6.405g

Sodium azide

0.5g

NADH.Na 2 .3H 2 o

0.475g

D-lactate dehydrogenase

7.2ku

pure water

1000mL

[0028] Add 8g / L surfactant Triton X-100 during the mixing process of the above materials to effectively control the generation of air bubbles during the particle dissolution process.

[0029] At the same time, adding exc...

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Abstract

The invention discloses a method for preparing a solid granular biochemical reagent, solving the problem that the conventional biochemical reagent cannot be accurately metered under the condition of zero gravity or bumping. The method mainly comprises the following steps of: (1) preparing the biochemical reagent; (2) distributing liquid drops through a liquid dispensing pump; (3) dropping the liquid drops into liquid nitrogen so that the liquid nitrogen in contact with the liquid drops absorbs heat so as to be gasified to form a gas flow, wherein the gas flow enables the liquid drops to be floated on the surface of the liquid nitrogen; and when the liquid drops are completely solidified, the liquid drops are formed into ice pellets and sunk to the bottom of the liquid nitrogen; and (4) freeze-drying the ice pellets in a freeze dryer, thereby obtaining the finished product. The method is high in accuracy, simple and convenient for operation, good in detection result and the like.

Description

technical field [0001] The invention relates to a preparation method of a biochemical reagent, in particular to a preparation method of a solid granular biochemical reagent. Background technique [0002] Biochemical analysis is the most basic and necessary diagnostic method in laboratory medicine in medical institutions at all levels. In this analysis process, biochemical reagents corresponding to various inspection items must be used. The biochemical reagents used in conventional biochemical analysis are generally liquid, solid powder after mechanical mixing, and freeze-dried reagents using ordinary freeze-drying processes. kind of form. The two forms of biochemical reagents after production are to obtain a longer shelf life than liquid reagents, but they must be redissolved in water before final use and used as ordinary liquid biochemical reagents. [0003] However, in many special cases, such as: under the weightless conditions of the space shuttle or under the conditio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
Inventor 冉鹏
Owner CHENGDU SEAMATY TECH
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