Sucrose-glucose-fructose content determination kit and determination method thereof
A measurement method and kit technology, applied in biochemical equipment and methods, microbial measurement/inspection, color/spectral characteristic measurement, etc., can solve the problems of easy error and high cost of the detection method, and achieve simple and environmentally friendly operation process. Accurate detection results and improved experimental efficiency
Pending Publication Date: 2020-03-27
苏州格锐思生物科技有限公司
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Problems solved by technology
[0004] Aiming at the deficiencies in the prior art, the invention provides a sucrose-glucose-fructose content assay kit and assay method thereof, which has a simple and convenient operation process and is environmentally friendly; the experimental results are more accurate; one kit can obtain three sugars (sucrose, glucose And fructose) content, improve the experimental efficiency and other advantages, solve the problem that common detection methods are prone to errors and high cost
Method used
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Embodiment Construction
[0040] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention.
[0041] see Figure 1-2 , a sucrose-glucose-fructose content determination kit, comprising a kit body 1, a reagent tube 2, a movable connection device 3, a sealing cover 4 and a locking device 5, characterized in that: the inside of the kit body 1 is fixedly equipped with a reagent Tube 2, the right side of the kit body 1 is fixedly equipped with a movable connection device 3, the movable connection device 3 includes a round rod fixed block, a round rod and a rotating block, the right side of the kit body 1 is fixedly installed with a round rod fixed block, and the round A round rod is fixedly installed on the opposite side of the rod fixing block, the surface of the round rod is movably connected with a rotating block, the right end of the movable connection device 3 is fixedl...
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The invention relates to the technical field of sugar content determination, and discloses a sucrose-glucose-fructose content determination kit and a determination method thereof. The sucrose-glucose-fructose content determination kit comprises a kit body, a reagent tube, a movable connecting device, a sealing cover and a locking device, wherein the reagent tube is fixedly arranged in the kit body; the movable connecting device is fixedly arranged on the right side of the kit body; and the sealing cover is fixedly arranged at the right end of the movable connecting device. According to the sucrose-glucose-fructose content determination kit and the determination method thereof, specific enzymes respectively act on sucrose, glucose and fructose, background interference of other substances ina sample is eliminated, and the detection result is accurate; and moreover, the three pieces of sugar data can be detected at one time, the experimental operation time is shortened, personal errors caused by batch detection are reduced, and a time-saving, money-saving and nontoxic detection means is provided for detection of a large number of samples.
Description
technical field [0001] The invention relates to the technical field of sugar content determination, in particular to a sucrose-glucose-fructose content determination kit and a determination method thereof. Background technique [0002] At present, most of the domestic biochemical methods for detecting sucrose, glucose and fructose are traditional acid hydrolysis methods, and finally react with chemical reagents such as anthrone or resorcinol for color development, but these common chemical color reagents have relatively low sensitivity and specificity. Low, and there will be high background values, resulting in high measured values. In addition, the detection process involves strong acids such as concentrated sulfuric acid, and improper operation will cause certain harm to the human body. [0003] Another method is to use high-performance liquid chromatography (HPLC) to detect, and requires an expensive differential detector, and it takes a long time to prepare the sample to...
Claims
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Login to View More IPC IPC(8): C12M1/00C12Q1/54C12Q1/533C12Q1/48C12Q1/34G01N21/33
CPCC12Q1/54C12Q1/533C12Q1/485C12Q1/34G01N21/33
Inventor 张彩霞苏艳孟赵凤林
Owner 苏州格锐思生物科技有限公司