The invention provides a method for determining microcystic toxins concentration and is based on that molybdenum disulfide quantum dots can interact with gold nanoparticles through the internal filtration effect. Microcystic toxins can affect the degree of aggregation of aptamer-modified gold nanoparticles in the high salt solution, and detection of the microcystic toxins concentration in the water environment is achieved. The method is advantaged in that upconversion fluorescence of the molybdenum disulfide used can effectively avoid interference of background matrix fluorescence in the waterenvironment, optical stability is good, the detection result is accurate and reliable, cost is low, operation is simple, environmental protection is realized, detection specificity is good, influenceof other common metal ions or anions on determination of the microcystic toxins is avoided, the anti-interference ability is strong, the result is sensitive and reliable, the detection limit is low,the response speed is fast, the entire reaction process takes only 20 minutes, detection efficiency is greatly improved, good stability is achieved, real-time online fast, specific detection can be realized, and the method can be used for simple and rapid detection of microcystic toxins in the actual water environment.