Method for determining microcystic toxins concentration

A technology of microcystin and concentration, which is applied in the direction of measuring devices, instruments, fluorescence/phosphorescence, etc., can solve the problems of low selectivity, high cost, and complicated operation of detection methods, and achieve simple operation, low detection limit, and avoid background The effect of fluorescence interference

Active Publication Date: 2019-04-30
YANGTZE NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a method for determining the concentration of microcystins, which solves the problems of complex operation, high cost and low selectivity of the existing detection methods.

Method used

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  • Method for determining microcystic toxins concentration
  • Method for determining microcystic toxins concentration
  • Method for determining microcystic toxins concentration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1) Preparation of molybdenum disulfide quantum dot stock solution with upconversion fluorescence:

[0038] Ammonium molybdate tetrahydrate, N-acetyl-L-cysteine ​​and thiourea were weighed according to the mass ratio of 3.16:1:0.39, and they were added to water and fully mixed under an ice bath to obtain a mixed solution, wherein, For each gram of sodium molybdate tetrahydrate, 64.7 mL of water needs to be added, and then the mixed solution is transferred to a hydrothermal reaction kettle for 4 hours at 200°C, cooled naturally, and then subjected to a centrifuge with a speed of 20,000 r / min. After centrifugation for 10 min, the supernatant was taken to obtain the original solution of molybdenum disulfide quantum dots.

[0039] 2) Draw a standard curve:

[0040] Take the microcystin nucleic acid aptamer solution with a concentration of 2 μM, the gold nanometer solution reduced with sodium borohydride with a concentration of 14.4 nM, the NaCl solution with a concentration...

Embodiment 2

[0048] 1) Preparation of molybdenum disulfide quantum dot stock solution with upconversion fluorescence:

[0049] Ammonium molybdate tetrahydrate, N-acetyl-L-cysteine ​​and thiourea were weighed according to the mass ratio of 3.16:1:0.39, and they were added to water and fully mixed under an ice bath to obtain a mixed solution, wherein, For each gram of sodium molybdate tetrahydrate, 64.7 mL of water needs to be added, and then the mixed solution is transferred to a hydrothermal reaction kettle for 4 hours at 220°C, cooled naturally, and then subjected to a centrifuge with a speed of 20,000 r / min. After centrifugation for 10 min, the supernatant was taken to obtain the original solution of molybdenum disulfide quantum dots.

[0050] 2) Draw a standard curve:

[0051] Take 5 μL of 2 μM microcystin nucleic acid aptamer solution, 100 μL of 14.4 nM sodium borohydride-reduced gold nano-solution and 30 μL of 100 mM KCl solution to obtain a mixed solution, and then add to the above ...

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Abstract

The invention provides a method for determining microcystic toxins concentration and is based on that molybdenum disulfide quantum dots can interact with gold nanoparticles through the internal filtration effect. Microcystic toxins can affect the degree of aggregation of aptamer-modified gold nanoparticles in the high salt solution, and detection of the microcystic toxins concentration in the water environment is achieved. The method is advantaged in that upconversion fluorescence of the molybdenum disulfide used can effectively avoid interference of background matrix fluorescence in the waterenvironment, optical stability is good, the detection result is accurate and reliable, cost is low, operation is simple, environmental protection is realized, detection specificity is good, influenceof other common metal ions or anions on determination of the microcystic toxins is avoided, the anti-interference ability is strong, the result is sensitive and reliable, the detection limit is low,the response speed is fast, the entire reaction process takes only 20 minutes, detection efficiency is greatly improved, good stability is achieved, real-time online fast, specific detection can be realized, and the method can be used for simple and rapid detection of microcystic toxins in the actual water environment.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a method for measuring the concentration of microcystins based on up-conversion fluorescence molybdenum disulfide quantum dots. Background technique [0002] Microcystins (MCs) are a class of cyclic heptapeptide hepatotoxins released by harmful cyanobacteria blooms. They have strong carcinogenic effects and are important environmental factors that induce liver cancer, gastroenteritis and other diseases. In view of the toxicity and harm of MCs, the World Health Organization (WHO) has listed MCs as harmful pollutants to be controlled in drinking water. MCs are listed together with hepatitis virus and aflatoxin as the three major environmental risk factors that induce the high incidence of primary liver cancer in southern my country. In this regard, the newly promulgated "Hygienic Standards for Drinking Water" (GB5749-2006) in 2006 The content of MCs is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 曹海燕石文兵董文飞陈果
Owner YANGTZE NORMAL UNIVERSITY
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