Unlock instant, AI-driven research and patent intelligence for your innovation.
A method for measuring microcystin concentration
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A microcystin and concentration technology, applied in the field of environmental protection, can solve the problems of low selectivity, high cost, and complicated operation of the detection method, and achieve the effect of simple operation, low detection limit, and avoiding background fluorescence interference
Active Publication Date: 2021-07-27
YANGTZE NORMAL UNIVERSITY
View PDF12 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0004] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a method for determining the concentration of microcystins, which solves the problems of complex operation, high cost and low selectivity of the existing detection methods.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0037] 1) Preparation of molybdenum disulfide quantum dot stock solution with upconversion fluorescence:
[0038] Ammonium molybdate tetrahydrate, N-acetyl-L-cysteine and thiourea were weighed according to the mass ratio of 3.16:1:0.39, and they were added to water and fully mixed under an ice bath to obtain a mixed solution, wherein, For each gram of sodium molybdate tetrahydrate, 64.7 mL of water needs to be added, and then the mixed solution is transferred to a hydrothermal reaction kettle for 4 hours at 200°C, cooled naturally, and then subjected to a centrifuge with a speed of 20,000 r / min. After centrifugation for 10 min, the supernatant was taken to obtain the original solution of molybdenum disulfide quantum dots.
[0039] 2) Draw a standard curve:
[0040] Take the microcystin nucleic acid aptamer solution with a concentration of 2 μM, the gold nanometer solution reduced with sodium borohydride with a concentration of 14.4 nM, the NaCl solution with a concentration...
Embodiment 2
[0048] 1) Preparation of molybdenum disulfide quantum dot stock solution with upconversion fluorescence:
[0049] Ammonium molybdate tetrahydrate, N-acetyl-L-cysteine and thiourea were weighed according to the mass ratio of 3.16:1:0.39, and they were added to water and fully mixed under an ice bath to obtain a mixed solution, wherein, For each gram of sodium molybdate tetrahydrate, 64.7 mL of water needs to be added, and then the mixed solution is transferred to a hydrothermal reaction kettle for 4 hours at 220°C, cooled naturally, and then subjected to a centrifuge with a speed of 20,000 r / min. After centrifugation for 10 min, the supernatant was taken to obtain the original solution of molybdenum disulfide quantum dots.
[0050] 2) Draw a standard curve:
[0051] Take 5 μL of 2 μM microcystin nucleic acid aptamer solution, 100 μL of 14.4 nM sodium borohydride-reduced gold nano-solution and 30 μL of 100 mM KCl solution to obtain a mixed solution, and then add to the above ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The present invention provides a method for determining the concentration of microcystins, based on the fact that molybdenum disulfide quantum dots can interact with gold nanoparticles through the inner filter effect, but microcystins can affect the aptamer-modified gold nanoparticles in The degree of aggregation and dispersion in high-salt solution realizes the detection of microcystin concentration in water environment. The up-conversion fluorescence of molybdenum disulfide used in the present invention can effectively avoid the interference of background matrix fluorescence in the water environment, has good optical stability, accurate and reliable detection results, low cost, simple operation, environmental protection, and good detection specificity. Avoid the influence of other common metal ions or anions on the determination of microcystins, strong anti-interference ability, sensitive and reliable results, low detection limit, fast response speed, the whole reaction process only takes 20 minutes, which greatly improves the detection efficiency and is stable It has good performance, can realize rapid and specific detection on-line in real time, and can be used for simple and rapid detection of microcystins in actual water environment.
Description
technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a method for measuring the concentration of microcystins based on up-conversion fluorescence molybdenum disulfide quantum dots. Background technique [0002] Microcystins (MCs) are a class of cyclic heptapeptide hepatotoxins released by harmful cyanobacteria blooms. They have strong carcinogenic effects and are important environmental factors that induce liver cancer, gastroenteritis and other diseases. In view of the toxicity and harm of MCs, the World Health Organization (WHO) has listed MCs as harmful pollutants to be controlled in drinking water. MCs are listed together with hepatitis virus and aflatoxin as the three major environmental risk factors that induce the high incidence of primary liver cancer in southern my country. In this regard, the newly promulgated "Hygienic Standards for Drinking Water" (GB5749-2006) in 2006 The content of MCs is ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More 
