A solid-state up-conversion fluorescent probe and its preparation method and application

A fluorescent probe and solid-state technology, applied in the field of chemical detection, can solve the problems of low luminous efficiency of up-conversion nanoparticles, affecting the sensitivity and detection limit of biological detection, and low luminous efficiency

Active Publication Date: 2020-10-27
DALIAN MARITIME UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still two main problems in the process of its practical application: one is that the fluorescent probe is unstable: most of the up-conversion fluorescence detection reported in the past is carried out in a liquid phase environment, and the nanoparticle solution used as a fluorescent probe Concentration and dispersion of particles in solution will affect the stability of fluorescent probes; second, the luminous efficiency of upconversion nanoparticles is low: the electronic transition of rare earth ions mainly comes from the 4f-4f transition of the inner shell, resulting in a small absorption cross section At the same time, it is inevitable to introduce some phonon vibration functional groups during the synthesis of upconversion nanoparticles, resulting in low luminous efficiency, which will seriously affect the sensitivity and detection limit of biological detection.

Method used

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  • A solid-state up-conversion fluorescent probe and its preparation method and application
  • A solid-state up-conversion fluorescent probe and its preparation method and application
  • A solid-state up-conversion fluorescent probe and its preparation method and application

Examples

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Embodiment 1

[0027] Example 1 Preparation of solid-state up-conversion fluorescent probe and its use in the detection of carcinoembryonic antigen (CEA)

[0028] Disperse the PMMA microspheres in water, insert the glass substrate vertically into the turbid liquid of PMMA microspheres, and then place it in an oven at 28°C for 30 hours. The PMMA microspheres were assembled on the glass substrate orderly by the surface tension during the solution volatilization process. Then, the PMMA photonic crystal was placed in an oven at 120 °C for 40 min to increase its mechanical strength.

[0029] 0.4g polyetherimide (PEI), 2.5mmolNaCl, 0.798mmolY (NO 3 ) 3 , 0.2mmol Yb(NO 3 ) 3 and 0.002mmol Tm(NO 3 ) 3 Place in a beaker, add 15ml ethylene glycol and stir well, weigh 4mmol NH 4 F powder in another beaker, add 10ml ethylene glycol, and stir; when the two samples are stirred to a clear solution, add the latter dropwise to the former and continue to stir until clarified and put into the reaction k...

Embodiment 2

[0033] Example 2 Preparation of solid-state up-conversion fluorescent probe and its use in the detection of carbohydrate antigen (CA-125)

[0034] Disperse the PS microspheres in water, then insert the glass slide vertically into the turbid solution of PS microspheres, and then place it in an oven at 32°C for 24 hours. The PS microspheres were assembled on the glass substrate orderly by the surface tension during the solution volatilization process. Then, the PS photonic crystal was placed in an oven at 110 °C for 40 min to increase its mechanical strength.

[0035] 0.4g polyetherimide (PEI), 2.5mmolNaCl, 0.78mmolY(NO 3 ) 3 , 0.2mmol Yb(NO 3 ) 3 and 0.02mmol Er(NO 3 ) 3 Place in a beaker, add 15ml ethylene glycol and stir well, weigh 4mmol NH 4 Put F powder in another beaker, add 10ml of ethylene glycol, and stir; when the two samples are stirred to a clear solution, add the latter dropwise to the former and continue stirring for 10 minutes, then put it into the reactio...

Embodiment 3

[0040] SiO 2 The microspheres were dispersed in water, and then the glass slide was inserted vertically into the SiO 2 The turbid solution of the microspheres was then placed in an oven at 40°C for 20 hours. The surface tension of SiO during solution volatilization 2 The microspheres are orderedly assembled on the glass substrate. Then, the SiO 2 The photonic crystals were placed in an oven at 100°C for 60 min to increase their mechanical strength.

[0041] 0.4g polyetherimide (PEI), 2.5mmolNaCl, 0.79mmolY(NO 3 ) 3 , 0.18mmol Yb(NO 3 ) 3 and 0.03mmol Er(NO 3 ) 3 Place in a beaker, add 15ml ethylene glycol and stir well, weigh 4mmol NH 4 Put F powder in another beaker, add 10ml of ethylene glycol, and stir; when the two samples are stirred to a clear solution, add the latter dropwise to the former and continue to stir for 10 minutes, then put it into the reaction kettle, and react 2 samples at 240°C Hours, after the reaction kettle was naturally cooled to room temper...

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Abstract

The invention relates to a solid state up-conversion fluorescence probe and a preparation method and an application thereof. The method comprises the following steps: a) preparing opal photonic crystal; b) preparing up-conversion nanoparticles; c) performing up-conversion nanoparticles functionalization modification; and d) compositing the up-conversion nanoparticles and the opal photonic crystal to prepare the up-conversion fluorescence probe. The invention also discloses the solid state up-conversion fluorescence probe prepared by the above method and a method by using the up-conversion fluorescence probe for detecting a tumor marker. The method uses photonic crystal effect for regulation and control of luminescence to enhance the up-conversion luminescence intensity, increase the detection sensitivity, and reduce the detection lower limit. The up-conversion fluorescence probe is excitant based on near infrared source, can overcome the background fluorescence interference; and can avoid the interference on the detection due to factors of solution concentration and dispersibility in a liquid state fluorescence probe.

Description

technical field [0001] The invention relates to the technical field of chemical detection, in particular to a solid-state up-conversion fluorescent probe and its preparation method and application. Background technique [0002] Cancer is one of the most important diseases that threaten human life and health. According to the "2015 China Cancer Registration Annual Report" released by the National Cancer Registration Center, there were about 4.29 million new cancer cases in my country in 2015, and an average of 8 people were diagnosed with cancer every minute. Cancer, 5 people died of cancer, and the incidence of malignant tumors is also increasing year by year and younger. The most important reason for the increase in the incidence and mortality of malignant tumors is that most malignant tumor patients have no obvious clinical manifestations in the early stage of the disease. According to the statistics of the World Health Organization, the cure rate of early cancers can reac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 徐赛陈宝玖高跃峰李香萍张金苏
Owner DALIAN MARITIME UNIVERSITY
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