A fluorescent hydrogel and its application in the detection of carbaryl

A hydrogel and carbaryl technology, applied in the field of biosensors, can solve problems such as interference, achieve high stability and selectivity, and avoid background fluorescence interference.

Active Publication Date: 2022-04-01
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This will inevitably be interfered by the matrix in complex biological samples (such as proteins and pigments, etc.), resulting in false positive responses
In addition, most fluorescence images are collected under fully exposed UV lasers. If cut-off filters are not introduced, they will be greatly interfered by excitation light and ambient light.
Therefore, based on the existing portable detection equipment, it is still a challenge to construct a portable, low-background sensor to achieve high-performance pesticide detection, which will provide a new perspective for food and environmental safety monitoring

Method used

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  • A fluorescent hydrogel and its application in the detection of carbaryl
  • A fluorescent hydrogel and its application in the detection of carbaryl
  • A fluorescent hydrogel and its application in the detection of carbaryl

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Weigh 0.37980g erbium trichloride hexahydrate and 0.00192g thulium trichloride hexahydrate and join in the three-neck round bottom flask of 50mL, then add 6mL oleic acid and 15mL 1-octadecene to it, at 700r min -1 Nitrogen was introduced under stirring for 30 minutes to remove oxygen, and then heated to 160°C and continued to stir for 25 minutes until a pale pink precursor solution was formed; after the precursor solution was cooled, 10 mL of methanol solution containing 0.1 g of sodium hydroxide and Add dropwise to the above solution, heat to 75°C to remove methanol; heat the system to 300°C for 90 minutes, and then -1 Centrifuge at a rotating speed, wash with a mixture of cyclohexane and ethanol (the volume ratio of cyclohexane and ethanol is 3:1) for 4 times, and then dissolve in cyclohexane to obtain solution A. Weigh 0.15168g of yttrium trichloride hexahydrate and add it into 6mL of oleic acid and 15mL of 1-octadecene, stir for 30 minutes under nitrogen protection,...

Embodiment 2

[0020] Embodiment 2: a kind of based on NaErF 4 :0.5%Tm 3+ @NaYF 4 The preparation method of / PDA fluorescent hydrogel and its application in carbaryl detection comprise the following steps:

[0021] 50μL, 20ng mL -1 Carbaryl standard solution with 50μL, 2.5U L -1 The AChE was reacted at 37 °C for 30 min. It is added to the NaErF that contains embodiment 1 preparation 4 :0.5%Tm 3+ @NaYF 4 / PDA fluorescent hydrogel cuvette, react at 37°C for 30 minutes. Fluorescent images of different carbaryl concentrations were obtained by irradiating the cuvette with a small 980nm laser, and were analyzed and processed by ImageJ software. At the concentration of carbaryl in this example, the corresponding inhibition rate of the sample was 22.89%.

Embodiment 3

[0022] Embodiment 3: a kind of based on NaErF 4 :0.5%Tm 3+ @NaYF 4 The preparation method of / PDA fluorescent hydrogel and its application in carbaryl detection comprise the following steps:

[0023] 50μL, 100ng mL -1 Carbaryl standard solution with 50μL, 2.5U L -1 The AChE was reacted at 37 °C for 30 min. It is added to the NaErF that contains embodiment 1 preparation 4 :0.5%Tm 3+ @NaYF 4 / PDA fluorescent hydrogel cuvette, react at 37°C for 30 minutes. Fluorescent images of different carbaryl concentrations were obtained by irradiating the cuvette with a small 980nm laser, and were analyzed and processed by ImageJ software. Under the concentration of carbaryl in this example, the corresponding inhibition rate of the sample is 56.54%.

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Abstract

A NaErF-based 4 :0.5%Tm 3+ @NaYF 4 The fluorescent hydrogel of / PDA and its application in the detection of carbaryl belong to the technical field of biosensors. Based on prepared NaErF 4 :0.5%Tm 3+ @NaYF 4 / Polydopamine (PDA) fluorescent hydrogel, in which PDA can act as NaErF 4 :0.5%Tm 3+ @NaYF 4 effective quencher. After the introduction of acetylcholinesterase, its catalytic product has strong antioxidant properties and prevents the polymerization of dopamine, thereby restoring the fluorescence signal. The quantitative detection of pesticides in tea was achieved by introducing carbaryl to modulate the fluorescence response signal. Based on this principle, the portable detection of carbaryl in actual samples was further combined with fluorescence image analysis. The invention has the advantages of low background interference, low cost, portability and the like, and provides a new perspective for portable monitoring of food, environment and public safety based on near-infrared nanoprobes.

Description

technical field [0001] The invention belongs to the technical field of biosensors, specifically a NaErF-based 4 :0.5%Tm 3+ @NaYF 4 / PDA fluorescent hydrogel and its application in carbaryl detection. Background technique [0002] Carbamate pesticides can effectively prevent and control pests and diseases, and play an important role in improving the output of agriculture, forestry and animal husbandry. Although carbamate pesticides have the characteristics of low toxicity and short half-life, irrational use and long-term bioaccumulation will cause serious environmental and life safety hazards. Its toxicity mechanism is similar to that of organophosphorus pesticides, that is, it can effectively inhibit the activity of cholinesterase and affect biological nerve conduction. Exposure to carbamate pesticides can cause cerebral edema, coma, and respiratory depression. Because it can be converted into nitrosobenzene compounds under acidic conditions, the International Agency fo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/85C09K11/02B01J13/00G01N21/64
CPCC09K11/7773C09K11/02C09K11/025B01J13/0065G01N21/6428G01N2021/6432
Inventor 卢革宇闫旭苏丹丹刘晓敏王晨光贾晓腾
Owner JILIN UNIV
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