33results about How to "Improve the signal-to-background ratio" patented technology

An optical scanning system and method for detecting anomalies, including pattern defects and particulate contaminants, on both patterned and unpatterned surfaces, using a light beam, scanning at a grazing angle with respect to the surfaces, a plurality of detectors and an interchannel communication scheme to compare data from each detector, which facilitates characterizing anomalies. The light beam illuminates a spot on the surface which is scanned over a short scan-line. The surface is moved in a manner so that the spot is scanned over its entire area in a serpentine fashion along adjacent striped regions. The plurality of detectors include groups of collector channels disposed circumferentially around the surface, a bright field reflectivity / autoposition channel, an alignment / registration channel and an imaging channel. The collector channels in each group are symmetrically disposed, in the azimuth, on opposite sides of the center of the scan line. The position of the collector channels, as well as the polarization of the beam, facilitates distinguishing pattern defects from particulate contaminants. The bright field reflectivity / autoposition channel is positioned to receive specularly reflected light that carries information concerning local variation in reflectivity, which is used to classify detected anomalies, as well as determine variations in the height of the surface. The alignment / registration channel is positioned to detect a maximum of the light scattered from the pattern on the surface to ensure that the streets of die present on the surface are oriented so as not to be oblique with respect to the scan line. The imaging channel combines the advantages of a scanning system and an imaging system while improving signal / background ratio of the present system.

The object of the disclosure is to measure temperature using pyrometers, in a simple and economic way, enabling precise temperature measurement, even for low temperatures. The disclosure presents an apparatus and method for thermally treating substrates, wherein the substrate is exposed to at least a first and at least a second radiation; the predetermined wavelengths of the first radiation are absorbed between the first radiation source and the substrate; a radiation from the substrate is measured in the predetermined wavelength using a radiation detector arranged on the same side as a second radiation source; the second radiation from the second radiation source is modulated and determined.

Provided are a fluorescent on / off switchable compound for a gene analysis, which is highly stable and highly sensitive, and enables amplification and observation of a trace gene signal, and a labeling reagent for detection of a bio-related material, which uses the fluorescent on / off switchable compound. A compound represented by the following formula (I′):wherein X is a hydrogen atom or a carboxylic acid-protecting group, Y is a 2,4-dinitrobenzenesulfonyl group, a 2-cyano-4-nitrobenzenesulfonyl group or a 5-nitropyridin-2-ylsulfonyl group, R1, R2, R3 and R4 are each independently a hydrogen atom, a halogen atom, a hydroxyl group, an alkyl group having a carbon number of 1 to 6, an alkoxy group having a carbon number of 1 to 6, an aryl group having a carbon number of 6 to 10, or a cyano group.

The invention relates to a method for improving the detection sensitivity in homogenous TR-FRET based bioaffinity assays. The sensitivity is improved by the use of long lifetime donors together with a high energy transfer efficiency and by carrying out the detection of the energy transfer based emission of the acceptor in a time window which is opened after a delay of 1 microsecond or more, but less than 50 microseconds, calculated from the donor excitation, and which time window has a width of 1 microsecond or more, but less than 100 microseconds. The invention concerns also the use of the improved method in multianalyte assays. Furthermore, the invention concerns a device suitable for carrying out the improved method.

A method of enhancing the fluorescence of and / or producing an increased Stokes' shift in a fluorescent dye comprising combining the dye with a base and / or a detergent is described. The method is suitable in chemical or biochemical techniques using fluorescent dyes, particularly techniques requiring staining or labeling of organic molecules, such as electrophoresis.

A sample substrate adapted for use with electromagnetic excitation light includes a base and a layer system. The layer system includes a multilayer interference coating with at least two layers wherein the thicknesses of the layers ensure that light emitted by a fluorescent sample material disposed on top of said multilayer interference coating is reflected. Light directed to a fluorescent sample material disposed on the substrate causes light to be emitted from the sample. The layer system includes a multilayer interference coating with at least two layers wherein thicknesses of the layers cause separation of the excitation light from the emitted light.

A micro beam generation unit capable of simultaneously capturing anisotropic images in a high signal-to-background ratio with a compact configuration and an X-ray small-angle scattering apparatus are provided. A micro beam generation unit 110 generates X-rays having a micro spot size, with which a sample is irradiated, in order to detect diffracted X-rays by a one-dimensional detector or a two-dimensional detector. The micro beam generation unit 110 includes a slit 115 that is provided on an X-ray optical path and reshapes X-rays into parallel beams, and two channel-cut monochromator crystals 117 and 118 that are arranged in arrangement of (+, −, −, +) and remove parasitic scattering of parallel beams reshaped by the slit. Accordingly, it is possible to simultaneously obtain anisotropic images in a high signal-to-background ratio with a compact configuration.

The present invention relates to a method for detecting renal disease and for assessing the efficacy of dialysis treatment using imaging agents having desirable in vivo properties.

A switch-type liposome nanometer fluorescent probe comprises a near-infrared fluorescent dye and a liposome phospholipid membrane wrapped outside it; the liposome phospholipid membrane is composed of choline derivatives, cholesterol and distearoylphosphatidylethanolamine- Composed of polyethylene glycol 2000; the switch-type liposome nano-fluorescent probe encapsulates the hydrophobic dye in the liposome inner core, and compared with the traditional method of loading the hydrophobic dye on the lipid bilayer, the loading rate of the dye is higher High, better stability, and combined with the advantages of fluorescence can be activated. The preparation process of the probe is simple, the cost is low, the use is convenient, and the imaging effect in the body is good.

The invention discloses a total internal reflection illumination and semi-total internal reflection illumination dual optical path fluorescence microscope system. A beam-splitter plate I is arranged on the light path of exciting light and the angle between the beam-splitter plate I and the light path is 135 degrees so that the exciting light is divided into a vertical transmission light and a vertical reflected light; the transmission light is reflected through a reflector so as to be parallel to the reflected light; the light paths of the reflected light and the transmission light are respectively and sequentially provided with a graded-density neutral filter, a convex lens I, a convex lens II and a light spot aperture regulator; the reflected light and the transmission light then form two vertical light paths by adjusting transmission directions through the reflector; convex lens III are respectively arranged on the mutually vertical light paths of the reflected light and the transmission light; the reflected light and the transmission light pass through the convex lens III so as to be incident to a beam-splitter plate II having an angle of 135 degrees with a light path of the reflected light; and two parallel lights divided through the beam-splitter plate II are incident to an exciting light inlet of a microscope. The total internal reflection illumination and semi-total internal reflection illumination dual optical path fluorescence microscope system can be used for bleaching fluorescence molecule of a cell membrane area, background fluorescence interference caused when the fluorescence molecule of the cell membrane area images a focal plane in a cell is avoided, and a signal background ratio of semi-total internal reflection fluorescence imaging in the cell is further improved.