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Ratio fluorescence nano probe, and preparation method and application thereof

A nano-probe and ratiometric fluorescence technology, which is applied in the fields of biomedical detection and biochemical analysis, can solve the problems of poor cell permeability, low sensitivity, and poor water solubility of quinoline derivatives, etc., to achieve sensitive detection, improve accuracy, and eliminate Background Interference Effects

Active Publication Date: 2014-09-10
SUN YAT SEN UNIV
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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the defects of poor water solubility and poor cell permeability of quinoline derivatives in the existing zinc ion fluorescence detection method, and the single emission detection method is easily interfered by complex matrix background and low sensitivity, and provides a Dual Emission Based Ratiometric Fluorescence Detection Method

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  • Ratio fluorescence nano probe, and preparation method and application thereof
  • Ratio fluorescence nano probe, and preparation method and application thereof
  • Ratio fluorescence nano probe, and preparation method and application thereof

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[0026] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments. Unless otherwise specified, the reagents, equipment and methods used in the present invention are commercially available reagents, equipment and routinely used methods in this technical field.

[0027] like figure 1 Shown, the present invention first synthesized 8-chloroacetyl aminoquinoline by chemical method, then prepared water-soluble zinc ion recognition probe ( PEI-Q), and finally we covalently attached this water-soluble zinc ion recognition probe to the surface of bipyridyl ruthenium-embedded silica fluorescent nanoparticles to prepare a novel ratiometric for zinc ion detection Fluorescent nanoprobes.

[0028] (1) Synthesis of 8-chloroacetylaminoquinoline: First, accurately weigh 288 mg of 8-aminoquinoline in a 25 mL round-bottomed flask, then add 202 mg of triethylamine and 10 mL of dichloromethane into it, and store at room ...

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Abstract

The invention relates to the fields of biomedical detection and biochemical analysis, and particularly discloses a novel fluorescence probe of surface-functionalized silicon dioxide fluorescence nanoparticles for intracellular / extracellular zinc ion detection. The probe is prepared by covalently connecting the aminoquinoline-grafted dendriform polyethyleneimine derivatives to the surface of the dipyridyl-ruthenium-embedded silicon dioxide fluorescence nanoparticles; and a ratio fluorescence method is utilized to perform intracellular / extracellular zinc ion detection. The method is an improvement on the basis of the zinc ion fluorescence detection method, and can maximally enhance the detection sensitivity.

Description

technical field [0001] The invention relates to the fields of biomedical detection and biochemical analysis, and more specifically relates to a ratiometric fluorescent nanoprobe and its preparation method and application. Background technique [0002] Zinc ions are the second most abundant transition metal ions in the human body after iron ions. It mainly exists in the form of divalent cations in biological processes and plays a very important role in the whole biological system. Studies have shown that zinc ions can be combined with some specific proteins, as cofactors and catalytic centers of their structures, and play the role of enzymes. In addition, biological processes such as brain function, gene transfer, immune function, and nerve signal transduction are all related to zinc ions. Therefore, the detection of zinc ions has become an important research direction of biological analysis, and the rapid and sensitive detection of zinc ions is of great clinical significanc...

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Application Information

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IPC IPC(8): C09K11/06G01N21/64
Inventor 易长青时宇鹏陈志华程鑫潘益张恒张肇敏
Owner SUN YAT SEN UNIV
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