Fluorescence ratio type spectral analysis method for detecting carcinoembryonic antigen, and application thereof

A carcinoembryonic antigen and spectral analysis technology, which is applied in the direction of fluorescence/phosphorescence, material analysis and material analysis through optical means, can solve problems such as interference of luminous intensity, achieve high sensitivity, eliminate background interference, improve accuracy and The effect of stability

Active Publication Date: 2019-12-24
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the analytical sensors constructed with afterglow nanomaterials are based on the change of a single luminescent signal to evaluate the content of the target substance in the sample to be detected, which cannot avoid the interference of certain factors in the detection environment on the luminous intensity.

Method used

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  • Fluorescence ratio type spectral analysis method for detecting carcinoembryonic antigen, and application thereof
  • Fluorescence ratio type spectral analysis method for detecting carcinoembryonic antigen, and application thereof
  • Fluorescence ratio type spectral analysis method for detecting carcinoembryonic antigen, and application thereof

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Effect test

Embodiment 1

[0036] A fluorescence ratio spectral analysis method for detecting carcinoembryonic antigen, the analysis method includes the following steps:

[0037] (1) Synthesis of ZGO:Sr NRs and surface CEA-Apta modification:

[0038] ①Add 0.5g Zn(NO 3 ) 2 , 0.0008g Sr(NO 3 ) 2 Add the solid to 8mL ultrapure water, mix and stir evenly, and add 200μL of concentrated HNO with a concentration of 14mol / L 3 After mixing uniformly, a colorless and transparent solution 1 is formed; then the concentration of 1mL is 0.02g / mL Na 2 GeO 3 The solution was added dropwise to the above colorless and transparent solution 1, and the pH value of the solution was adjusted to 7 by adding ammonia water, and the solution gradually changed from clear to white and turbid. Stir continuously and quickly at room temperature for 1 hour, then transfer it to a PTFE hydrothermal reactor and heat it up to 200°C for 4 hours at a heating rate of 2°C / min. After cooling to room temperature, collect the lower sediment by centrifu...

Embodiment 2

[0045] A fluorescence ratio spectral analysis method for detecting carcinoembryonic antigen, the analysis method includes the following steps:

[0046] (1) Synthesis of ZGO:Sr NRs and CEA-Apta modification:

[0047] ①Add 0.6g Zn(NO 3 ) 2 , 0.001g Sr(NO 3 ) 2 Add the solids to 10mL of ultrapure water, mix and stir evenly, and add 300μL of concentrated HNO with a concentration of 15mol / L 3 After mixing uniformly, a colorless and transparent solution 1 is formed, and then the 2mL concentration is 0.06g / mL Na 2 GeO 3 The solution was added dropwise to the above colorless and transparent solution 1, and the pH value of the solution was adjusted to 9 by adding ammonia water. The solution gradually changed from clear to white and turbid. Stir continuously at room temperature for 1.5 hours and then transfer it to a PTFE hydrothermal reactor to heat up to 220°C for 5 hours at a heating rate of 3°C / min. After cooling to room temperature, collect the lower sediment by centrifugation for precip...

Embodiment 3

[0055] A fluorescence ratio spectral analysis method for detecting carcinoembryonic antigen, the analysis method includes the following steps:

[0056] (1) Synthesis of ZGO:Sr NRs and CEA-Apta modification:

[0057] ①Add 0.7g Zn(NO 3 ) 2 ,0.0012g Sr(NO 3 ) 2 Add the solids to 12mL of ultrapure water, mix and stir evenly, and add 400μL of 16mol / L concentrated HNO 3 After mixing uniformly, a colorless and transparent solution 1 is formed, and then 3mL is 0.1g / mL Na 2 GeO 3 The solution was added dropwise to the above colorless and transparent solution 1, and the pH value of the solution was adjusted to 11 by adding ammonia water. The solution gradually changed from clear to white and turbid. Stir continuously and rapidly at room temperature for 2 hours, then transfer it to a polytetrafluoroethylene hydrothermal reactor and heat up to 240°C for 6 hours at a heating rate of 4°C / min. After cooling to room temperature, the lower sediment was collected by centrifugation, the sediment was ...

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Abstract

The invention provides a fluorescence ratio type spectral analysis method for detecting carcinoembryonic antigen, and belongs to the technical field of optical analysis. The method provided by the invention comprises the main contents of the preparation of long afterglow nanorods ZGO: Sr NRs, the modification of surface carcinoembryonic antigen aptamers, and the preparation of ZGC@PDA nanomaterials. By using ZGC@PDA as both a reference signal and a quencher, and using a detection probe ZGO: Sr NRs that can specifically recognize carcinoembryonic antigens, a ratio-type spectroscopic analysis method that can be used to detect carcinoembryonic antigens is constructed. The analysis method has good accuracy and stability.

Description

Technical field [0001] The invention belongs to the field of optical analysis technology, and particularly relates to a fluorescence ratio spectrum analysis technology for detecting carcinoembryonic antigen. Background technique [0002] Carcinoembryonic antigen (CEA) is a common tumor marker for early diagnosis of colon cancer and rectal cancer. After a large number of clinical trials, it has been found that in addition to gastrointestinal tumors, the content of carcinoembryonic antigen can be increased, and some other cancers Such as breast cancer, lung cancer, etc. can also cause an increase in the concentration of carcinoembryonic antigen in serum. Therefore, carcinoembryonic antigen is considered to be a broad-spectrum biomarker. Although it cannot be used as a specific marker for judging a certain tumor disease, accurate detection of the level of carcinoembryonic antigen in the body is useful in the differential diagnosis of malignant tumors. Many aspects such as monitorin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N33/53G01N21/64B82Y20/00B82Y40/00B82Y30/00
CPCB82Y20/00B82Y30/00B82Y40/00G01N21/6428G01N21/6486G01N33/53G01N33/533G01N2021/6432
Inventor 赵媛施丽霞刘瀚
Owner JIANGNAN UNIV
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