Application of diamond-like carbon film used as matrix in laser desorption ionization mass spectra

A technology of ionization mass spectrometry and laser desorption, which is applied in the application field of laser desorption ionization mass spectrometry, can solve problems such as difficult to accurately control the amount of matrix added, weak carrier target surface effect, poor repeatability of detection signals, etc., to achieve quantitative analysis, Easy to operate and improve repeatability

Inactive Publication Date: 2010-09-29
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because these inorganic carbon materials are mostly solid powders, almost insoluble in water and general organic solvents, there are still some problems in the application as MALDI matrix: (1) it is difficult to accurately control the amount of matrix added, and the repeatability of operation is poor; (2) it is difficult to A uniform matrix layer is formed, the repeatability of the detection signal is poor, and it is difficult to carry out quantitative analysis; (3) the matrix layer is loose, and the interaction with the surface of the carrier target is weak, and it is easy to fly away from the target surface in the vacuum electric field of the ion source, contaminating the ion source

Method used

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  • Application of diamond-like carbon film used as matrix in laser desorption ionization mass spectra
  • Application of diamond-like carbon film used as matrix in laser desorption ionization mass spectra
  • Application of diamond-like carbon film used as matrix in laser desorption ionization mass spectra

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preparation example Construction

[0032] The diamond-like carbon film material is prepared by vacuum cathode arc deposition (vacuum cathodic arc deposition) method. In this method, graphite is used as the carbon source, and the graphite target is ablated by arc discharge in an inert gas to generate carbon atoms and carbon ions, and a dense, sp 3 Component DLC film. The diamond-like carbon film material prepared by this method has uniform pores, such as figure 2 As shown in the scanning electron microscope, the pore size is between 200nm and 50μm.

[0033] Preparation of diamond-like carbon film material

[0034] The graphite target is 99.99% high-purity graphite, and the sputtering gas is 99.99% high-purity argon, using monocrystalline silicon wafer (20mm×20mm), stainless steel sheet (100mm×100mm) or commercial MALDI target (100mm×150mm) as a carrier. First use analytical grade acetone and ethanol to ultrasonically clean the carrier for 10 minutes, take it out and dry it, and put it into a vacuum chamber....

Embodiment 1

[0040] Example 1 Analysis of low molecular weight compounds by laser desorption ionization mass spectrometry on the surface of diamond-like carbon film

[0041] 1. Preparation of sample solution: 3.2 mg of small peptide Phe-Ala, 3.0 mg of small peptide Gly-Val, and 2.9 mg of benzoyl-L-tyrosine ethyl ester hydrochloride (BTEE) were dissolved in 1 mL of 50 % methanol solution. Store in a 4°C refrigerator for later use.

[0042] 2. Laser desorption ionization mass spectrometry analysis on the surface of diamond-like carbon film: Take 1 μL of Phe-Ala, Gly-Val, and BTEE sample solutions, drop them on different positions on the surface of diamond-like carbon film, dry naturally, and use laser Desorption ionization mass spectrometry.

[0043] Analysis results:

[0044] Depend on image 3 It can be seen that the three low molecular weight compounds BTEE, Gly-Val and Phe-Ala have high desorption efficiency on the surface of diamond-like carbon film in the absence of conventional or...

Embodiment 2

[0045] Embodiment 2 uses the comparison of conventional organic matrix and diamond-like carbon film surface laser desorption ionization mass spectrometry analysis

[0046] 1. Preparation of sample solution: 2.9 mg of benzoyl-L-tyraminoethyl ester hydrochloride (BTEE) was dissolved in 1 mL of 50% methanol solution, and diluted with 50% methanol solution to 29 μg / mL and 290 ng / mL respectively. mL. Store in a 4°C refrigerator for later use.

[0047] 2. Laser desorption ionization mass spectrometry analysis on the surface of diamond-like carbon film: Take 1 μL of BTEE sample solution with a concentration of 29 μg / mL and 290 ng / mL, respectively, and drop them on different positions on the surface of diamond-like carbon film. After natural drying, use laser Desorption ionization mass spectrometry.

[0048] 3. Laser desorption ionization mass spectrometry analysis of conventional organic matrix: Take 1 μL of matrix solution (0.5 mg / mL) and spot on the surface of the metal target, a...

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Abstract

The invention relates to a laser desorption ionization mass spectra, in particular to application of diamond-like carbon film used as matrix in the laser desorption ionization mass spectra; the surface of the diamond-like carbon film is used as an assisting matrix of laser desorption ionization flying time mass spectra, the diamond-like carbon film has high desorption ionization efficiency to compounds with low molecular weight and can not generate background interference of matrix molecules, so as to realize high-efficiency and rapid detection to the compounds with low molecular weight; the laser desorption ionization flying time mass spectra which adopts the diamond-like carbon film as the assisting matrix is a new desorption ionization method without adding any organic matrix, so as toprovide a general, high-efficiency and rapid detection method for the compounds with low molecular weight such as natural products and ecological metabolites, promote the development of analysis of compounds with low molecular weight by the laser desorption ionization flying time mass spectra technology and find new field for the application of the diamond-like carbon film material.

Description

technical field [0001] The invention relates to desorption ionization mass spectrometry, in particular to the application of a diamond-like carbon film as a substrate in laser desorption ionization mass spectrometry. Background technique [0002] In 1988, Tanaka and Hillenkamp independently proposed the technology of using the matrix material to absorb laser energy to assist the analyte to enter the gas phase desorption ionization technology, called matrix assisted laser desorption ionization technology (Matrix Assisted Laser Desorption / Ionisation, MALDI). This technique coupled with time-of-flight mass spectrometry (MALDI-TOF MS) has become a powerful tool in biotechnology and has been successfully applied to the mass spectrometry detection of non-volatile and thermally unstable proteins, DNA / RNA, polysaccharides and other biomolecules , and won the 2002 Nobel Prize in Chemistry. The presence of matrix substances plays an extremely important role in energy transfer and mai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64
Inventor 邹汉法厉欣张宇
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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