Preparation method of silage leach liquor and application in silage detection

Abstract

The Invention discloses a preparation method of silage leach liquor, which is particularly practiced by mixing and immersing sterilized distilling water with silage; performing sonic oscillation afterlow-concentration hydrochloric acid treatment; filtering by a filter membrane, wherein the filter fluid is the silage leach liquor. Besides, the invention further provides an application of silage leach liquor prepared by the preparation method in the silage detection. Diluted hydrochloric acid with 1% of mass concentration is added during the preparation process of silage leach liquor; on the one hand, the low-concentration diluted hydrochloric acid can promote the complete dissolution of fermenting indexes in the silage such as organic acid and ammoniacal nitrogen; on the other hand, the viscosity between the silages is weakened after being treated by hydrochloric acid, the solid and fluid can be separated rapidly in the process of filtering by adopting the filter membrane, thereby greatly shortening the experimental operation time and saving labor force and lab consumables.

Description

technical field

[0001] The invention belongs to the technical field of forage silage detection, and in particular relates to a method for preparing silage extract and its application in silage detection. Background technique

[0002] Silage is a commonly used feed, which is made of plant feed with high moisture content after sealing and fermenting, and is mainly used to feed ruminants. Silage is more resistant to storage than fresh feed, and its nutritional content is stronger than dry feed. Plus, silage storage takes up less land and has no fire problems.

[0003] The pH value, organic acid, and ammoniacal nitrogen in silage are important technical indicators for evaluating the fermentation quality of silage. In order to detect these indicators, it is necessary to extract the silage to obtain an extract, which is reflected by testing the relevant indicators in the extract. Silage performance.

[0004] The preparation method of the traditional silage extract in the labora...

Examples

Embodiment 1

[0025] A method for preparing silage extract, specifically implemented according to the following steps:

[0026] Step 1: Add distilled water equivalent to 2 / 3 of the volume of the glass container into the glass container, seal the mouth of the glass container, then sterilize the glass container under high temperature and high pressure for 20 minutes, take it out after sterilization, and cool it down to room temperature naturally. Obtain sterilized distilled water;

[0027] Step 2, according to the mass ratio of sterilized distilled water to alfalfa silage of 8:1, add alfalfa silage into the sterilized distilled water of step 1 and seal and soak for 3 hours, and obtain the mixture after soaking;

[0028] Step 3, adding mass concentration to the mixture of step 2 is 1% hydrochloric acid solution, and the addition of hydrochloric acid solution is 1 / 20 of the quality of alfalfa silage, ultrasonic oscillation 3min after adding, wherein, the temperature of ultrasonic oscillation is...

Embodiment 2

[0031] A method for preparing silage extract, specifically implemented according to the following steps:

[0032] Step 1, add distilled water equivalent to 2 / 3 of the volume of the glass container into the glass container, seal the mouth of the glass container, and then sterilize the glass container under high temperature and high pressure for 25 minutes, take it out after the sterilization is completed, and cool it down to room temperature naturally. Obtain sterilized distilled water;

[0033] Step 2, according to the mass ratio of the sterilized distilled water and the Leymus chinensis silage being 9:1, the Leymus chinensis silage was added into the sterilized distilled water of the step 1 and sealed and soaked for 4 hours, and the mixture was obtained after soaking;

[0034] Step 3, add the hydrochloric acid solution that mass concentration is 1% to the mixture of step 2, and the addition amount of hydrochloric acid solution is 1 / 25 of the quality of Leymus chinensis silage...

Embodiment 3

[0037] A method for preparing silage extract, specifically implemented according to the following steps:

[0038] Step 1. Add distilled water equivalent to 2 / 3 of the volume of the glass container into the glass container, seal the mouth of the glass container, and then sterilize the glass container under high temperature and high pressure for 30 minutes. After the sterilization is completed, take it out and let it cool down to room temperature naturally. Obtain sterilized distilled water;

[0039] Step 2, according to the mass ratio of the sterilized distilled water and the stipa silage of 10:1, the stipa silage is added to the sterilized distilled water of step 1 and sealed and soaked for 5 hours, and the mixture is obtained after soaking;

[0040] Step 3, adding a mass concentration of 1% hydrochloric acid solution to the mixture in step 2, and the addition of the hydrochloric acid solution is 1 / 30 of the quality of Stipa silage silage, ultrasonic oscillation 5min after the...

technical field

[0001] The invention belongs to the technical field of forage silage detection, and in particular relates to a method for preparing silage extract and its application in silage detection. Background technique

[0002] Silage is a commonly used feed, which is made of plant feed with high moisture content after sealing and fermenting, and is mainly used to feed ruminants. Silage is more resistant to storage than fresh feed, and its nutritional content is stronger than dry feed. Plus, silage storage takes up less land and has no fire problems.

[0003] The pH value, organic acid, and ammoniacal nitrogen in silage are important technical indicators for evaluating the fermentation quality of silage. In order to detect these indicators, it is necessary to extract the silage to obtain an extract, which is reflected by testing the relevant indicators in the extract. Silage performance.

[0004] The preparation method of the traditional silage extract in the labora...

Examples

Embodiment 1

[0025] A method for preparing silage extract, specifically implemented according to the following steps:

[0026] Step 1: Add distilled water equivalent to 2 / 3 of the volume of the glass container into the glass container, seal the mouth of the glass container, then sterilize the glass container under high temperature and high pressure for 20 minutes, take it out after sterilization, and cool it down to room temperature naturally. Obtain sterilized distilled water;

[0027] Step 2, according to the mass ratio of sterilized distilled water to alfalfa silage of 8:1, add alfalfa silage into the sterilized distilled water of step 1 and seal and soak for 3 hours, and obtain the mixture after soaking;

[0028] Step 3, adding mass concentration to the mixture of step 2 is 1% hydrochloric acid solution, and the addition of hydrochloric acid solution is 1 / 20 of the quality of alfalfa silage, ultrasonic oscillation 3min after adding, wherein, the temperature of ultrasonic oscillation is...

Embodiment 2

[0031] A method for preparing silage extract, specifically implemented according to the following steps:

[0032] Step 1, add distilled water equivalent to 2 / 3 of the volume of the glass container into the glass container, seal the mouth of the glass container, and then sterilize the glass container under high temperature and high pressure for 25 minutes, take it out after the sterilization is completed, and cool it down to room temperature naturally. Obtain sterilized distilled water;

[0033] Step 2, according to the mass ratio of the sterilized distilled water and the Leymus chinensis silage being 9:1, the Leymus chinensis silage was added into the sterilized distilled water of the step 1 and sealed and soaked for 4 hours, and the mixture was obtained after soaking;

[0034] Step 3, add the hydrochloric acid solution that mass concentration is 1% to the mixture of step 2, and the addition amount of hydrochloric acid solution is 1 / 25 of the quality of Leymus chinensis silage...

Embodiment 3

[0037] A method for preparing silage extract, specifically implemented according to the following steps:

[0038] Step 1. Add distilled water equivalent to 2 / 3 of the volume of the glass container into the glass container, seal the mouth of the glass container, and then sterilize the glass container under high temperature and high pressure for 30 minutes. After the sterilization is completed, take it out and let it cool down to room temperature naturally. Obtain sterilized distilled water;

[0039] Step 2, according to the mass ratio of the sterilized distilled water and the stipa silage of 10:1, the stipa silage is added to the sterilized distilled water of step 1 and sealed and soaked for 5 hours, and the mixture is obtained after soaking;

[0040] Step 3, adding a mass concentration of 1% hydrochloric acid solution to the mixture in step 2, and the addition of the hydrochloric acid solution is 1 / 30 of the quality of Stipa silage silage, ultrasonic oscillation 5min after the...

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