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Metal toxicity or metal poisoning is the toxic effect of certain metals in certain forms and doses on life. Some metals are toxic when they form poisonous soluble compounds. Certain metals have no biological role, i.e. are not essential minerals, or are toxic when in a certain form. In the case of lead, any measurable amount may have negative health effects. Often heavy metals are thought as synonymous, but lighter metals may also be toxic in certain circumstances, such as beryllium and lithium. Not all heavy metals are particularly toxic, and some are essential, such as iron. The definition may also include trace elements when in abnormally high doses may be toxic. An option for treatment of metal poisoning may be chelation therapy, which is a technique which involves the administration of chelation agents to remove metals from the body.
A high-sensitivity rapid fluorescence probe without metal toxicity, a preparation method and application in food pathogenic bacteria detection
This invention discloses a metal-free, highly sensitive, rapid fluorescent probe, its preparation method, and its application in the detection of pathogenic bacteria in food, belonging to the field of food detection technology. The probe consists of an organic fluorescent molecular graphene composite matrix, an organic-inorganic hybrid dopant, and a specific recognition molecule. The dopant is carboxylated cellulose and silica nanoparticles, with a mass ratio of 1:6~10 to the composite matrix. The specific recognition molecule is a pathogenic bacteria aptamer, grafted onto the probe surface via spray coating. The probe triggers a fluorescence response by the specific binding of the aptamer to the target pathogenic bacteria, completing detection within 5 minutes with a sensitivity ≥90% and a specificity ≥98%. The probe contains no metal components, posing no toxicity or environmental risk, and is suitable for rapid on-site food detection and live animal detection. Simultaneously, the preparation cost is reduced by 90%, and the cost per sample is reduced by 40%, demonstrating promising application prospects.
Owner:SHANDONG NORMAL UNIV