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A determination method and application of nickel ion toxicity to wheat root elongation under hydroponic conditions

A determination method and technology for nickel ions, which can be used in material inspection products, measuring devices, instruments, etc., and can solve problems such as poor applicability and incomplete evaluation of heavy metal toxicity assessment methods.

Active Publication Date: 2018-03-02
NANJING UNIV
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Problems solved by technology

[0007] Aiming at the problems of incomplete assessment and poor applicability in existing heavy metal toxicity assessment methods, the present invention provides a determination method and application of nickel ion toxicity to wheat root elongation under hydroponic conditions. In soil solution, a relatively large amount of The cations are mainly K + 、Na + , Ca 2+ , Mg 2+ and H + At the same time, there are two forms of nickel ions that may produce biological toxicity in solution, namely Ni 2+ and NiHCO 3 +

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  • A determination method and application of nickel ion toxicity to wheat root elongation under hydroponic conditions
  • A determination method and application of nickel ion toxicity to wheat root elongation under hydroponic conditions
  • A determination method and application of nickel ion toxicity to wheat root elongation under hydroponic conditions

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Embodiment 1

[0082] For five competing cations (K + 、Na + , Ca 2+ , Mg 2+ and H + ) using the control variable method, only changing the concentration of a certain cation while controlling the concentration of other cations to remain unchanged, and each treatment group was repeated three times. The ionic components of the hydroponic solution in each treatment group are shown in Table 2. Before the experiment, the wheat seeds were bred, and the specific breeding method was as follows: the cleaned and sterilized wheat seeds were evenly placed in a petri dish soaked with ultrapure water containing filter paper, and the petri dish was placed in an artificial climate box. breeding in. The environmental parameters of the climate box are temperature 20°C, air humidity 80%, no light, and the breeding lasts for 48 hours, and the filter paper in the petri dish should be kept moist during the breeding period. After the 48h breeding was completed, the seeds with good germination condition and th...

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Abstract

The invention discloses a determination method for wheat root elongation toxicity of nickel ions under a hydroponic condition and application and belongs to the field of ecological heavy metal toxicity assessment. A control variable method is adopted, concentration of positive ions is controlled and changed separately, the inhibiting effects of five types of positive ions including K<+>, Na<+>, Ca<2+>, Mg<2+> and H<+> on nickel ion toxicity are calculated out step by step, meanwhile a MINTEQ program is utilized to calculate the amount of Ni<2+> and the amount of NiHCO3<+> under all conditions so as to calculate the toxic effects of two nickel forms, biological toxicity inhibition parameters of all the positive ions on Ni and toxic effect parameters of the two Ni forms are determined, the method for determining wheat root toxicity of Ni<2+> is established under the hydroponic condition, an obtained result can be used for prediction of wheat root toxicity of the Ni<2+> under the hydroponic condition, and a reference is made to soil heavy metal ecological risk assessment and soil contamination treatment.

Description

technical field [0001] The invention belongs to the field of ecological heavy metal toxicity evaluation, more specifically, relates to a method for determining the elongation toxicity of nickel ions to wheat roots under hydroponic conditions and its application, and is a method that considers the effects of cations and heavy metal ions on biofilm ligands. Competitive method to calculate biotoxicity. Background technique [0002] Nickel pollution is a kind of heavy metal pollution caused by excessive nickel and its compounds entering the environment. High concentrations of nickel in soil and water can have toxic effects on animals and plants, and food crops in soil containing high concentrations of nickel will have a toxic effect on nickel. There is a certain enrichment effect, and then it is eaten, which ultimately affects human life and health. As one of the three major grains in the world, wheat is a food crop widely grown in the world. Therefore, the prediction and esti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/50G01N33/00
CPCG01N33/0098G01N33/5097
Inventor 顾雪元姜洋童非谢锦宇王晓蓉
Owner NANJING UNIV
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