Broad-spectrum soil heavy metal passivation microbial inoculum as well as preparation method and application thereof

A heavy metal, broad-spectrum technology, applied in the direction of biochemical equipment and methods, chemical instruments and methods, applications, etc., can solve the problems of limited repair of heavy metals, reduce the toxicity of broad-spectrum heavy metals, and indirect passivation, so as to avoid Food safety issues, broad-spectrum heavy metal passivation effect, low price effect

Active Publication Date: 2022-04-29
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The bacterial agent can effectively solve the problems of limited types of heavy metals repaired by biological bacterial agents, indirect passivation, and large economic investment, so as to achieve efficient reduction of the toxicity of broad-spectrum heavy metals, and the removal rate of heavy metals in soil and rice has reached More than 70%, while reducing the toxicity of broad-spectrum heavy metals without affecting the physical and chemical properties of the soil, it has promoted the development of microbial remediation of heavy metal pollution technology

Method used

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  • Broad-spectrum soil heavy metal passivation microbial inoculum as well as preparation method and application thereof
  • Broad-spectrum soil heavy metal passivation microbial inoculum as well as preparation method and application thereof
  • Broad-spectrum soil heavy metal passivation microbial inoculum as well as preparation method and application thereof

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Effect test

Embodiment 1

[0090] Embodiment 1 a kind of broad-spectrum soil heavy metal passivation bacterial agent

[0091] In the present embodiment, the preparation method of broad-spectrum soil heavy metal passivation bacterial agent is:

[0092] (1) Activation strain: inoculate the above-mentioned Geobacter sulfurreducens HMP-1 into the above-mentioned NBAF liquid medium for activation.

[0093] (2) Bacterial liquid preparation: Inoculate the activated Geobacter sulfurreducens HMP-1 bacterial liquid into new NBAF liquid medium at an inoculation amount of 3% (v / v), and ferment anaerobically at 30°C for 7 days;

[0094] (3) Preparation of solid bacterial agent: the fermented bacterial liquid is vacuum-dried at low temperature to obtain Geobacter sulfurreducens HMP-1 bacterial powder, and then the ratio of the mass meter 1:35 is fully mixed with the Geobacter sulfurreducens HMP-1 bacterial powder and auxiliary materials (guarantee The number of live bacteria in the final product complies with the st...

Embodiment 2

[0099] Embodiment 2 A kind of broad-spectrum soil heavy metal passivation bacterial agent

[0100] In the present embodiment, the preparation method of broad-spectrum soil heavy metal passivation bacterial agent is:

[0101] (1) Activation strain: inoculate the above-mentioned Geobacter sulfurreducens HMP-1 into the above-mentioned NBAF liquid medium for activation.

[0102] (2) Bacterial liquid preparation: Inoculate the activated Geobacter sulfurreducens HMP-1 bacterial liquid into new NBAF liquid medium at an inoculation amount of 3% (v / v), and ferment anaerobically at 30°C for 7 days;

[0103] (3) Preparation of solid bacterial agent: the fermented bacterial liquid is vacuum-dried at low temperature to obtain Geobacter sulfurreducens HMP-1 bacterial powder, and then the ratio of the mass meter 1:40 is fully mixed with the Geobacter sulfurreducens HMP-1 bacterial powder and auxiliary materials (guarantee The number of live bacteria in the final product complies with the st...

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Abstract

The invention discloses a broad-spectrum soil heavy metal passivating microbial inoculum as well as a preparation method and application thereof. The broad-spectrum soil heavy metal passivating microbial inoculum contains Geobacter sulfurreducens HMP-1 and an auxiliary material. According to the broad-spectrum heavy metal passivation microbial inoculum, sulfydryl (-HS) rich on the surface of Geobacter sulfurreducens HMP-1 thalli directly forms metal sulfide minerals with heavy metal ions, so that the toxicity of heavy metal in the environment is reduced. The microbial agent can effectively solve the problems that the existing biological microbial agent is limited in heavy metal type remediation, not direct in passivation effect and large in economic investment, the removal rate of heavy metals in soil and rice reaches 70% or above, the toxicity of broad-spectrum heavy metals is reduced under the condition that the physical and chemical properties of the soil are not affected, and meanwhile, the cost is reduced. The development of a heavy metal pollution microbial remediation technology is promoted.

Description

technical field [0001] The invention belongs to the technical field of pollution treatment engineering, and in particular relates to a broad-spectrum soil heavy metal passivation bacterial agent and a preparation method and application thereof. Background technique [0002] According to the survey, heavy metal (Cd, Cu, Pb, Zn, Hg, etc.) pollution is one of the most important inorganic pollutants in farmland soil in the world. Because rice, wheat and various vegetables have a strong ability to absorb heavy metals, they are easy to absorb heavy metals in the soil and remain in plants. Long-term intake of these foods with excessive heavy metals will cause serious harm to the human body. Therefore, soil heavy metal pollution Restoration is an important problem that needs to be solved urgently to ensure food security and human health. [0003] At present, in the reports and practices of soil heavy metal remediation, chemical passivators can better fix heavy metals in soil. The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/74C12N15/65B09C1/10C09K17/40C12R1/01C09K101/00
CPCC12N1/20C12N15/74C12N15/65B09C1/10C09K17/40C09K2101/00Y02W30/40
Inventor 周顺桂靖宪月刘星
Owner FUJIAN AGRI & FORESTRY UNIV
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