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Bacillus tropicalis resistant to heavy metal degradation of waste engine oil

A technology of bacillus and waste engine oil, applied in the field of microorganisms, can solve the problems of heavy metal and petroleum hydrocarbon compound pollution, and achieve the effects of avoiding disadvantages and inapplicability, no secondary pollution, and easy operation

Active Publication Date: 2021-11-16
CHONGQING TECH & BUSINESS UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, part of the heavy metal-contaminated soil will also be accompanied by oil pollution caused by machining and other operations, resulting in compound pollution of heavy metals and petroleum hydrocarbons

Method used

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  • Bacillus tropicalis resistant to heavy metal degradation of waste engine oil
  • Bacillus tropicalis resistant to heavy metal degradation of waste engine oil
  • Bacillus tropicalis resistant to heavy metal degradation of waste engine oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Study on the maximum degradation ability of bacteria strains to waste engine oil.

[0057] In order to study the maximum degradation ability of Bacillus tropicalis for waste engine oil, the cell fermentation broth (OD 600nm =1.3±0.02) Inoculate 10% of the inoculum amount into 100mL inorganic salt medium containing different concentrations of waste engine oil (initial concentrations of waste engine oil are 488, 1017, 2089, 3070, 4000mg / L respectively), and the initial pH value is adjusted The temperature is 7.0, the temperature is 35°C, shake culture at 160r / min to carry out the shaking flask experiment of Bacillus tropicalis degrading waste engine oil, and monitor two indicators respectively: 1) the concentration of waste engine oil in the sample liquid and calculate the degradation rate of waste engine oil, the waste engine oil The level of oil degradation rate represents the strength of the degradation ability of the bacteria at this concentration; 2) Determination of...

Embodiment 2

[0059] Research on the ability of strains to tolerate Cr(Ⅵ), Cd(Ⅱ), Cu(Ⅱ) and Pb(Ⅱ).

[0060] To study the tolerance of Bacillus tropicalis to Cr(VI), Cd(II), Cu(II), and Pb(II), the cell fermentation broth (OD 600nm =1.3±0.02) inoculate 30mL LB medium (Cr(Ⅵ), Cu(Ⅱ) The actual concentration of Pb(Ⅱ) is 50mg / L~550mg / L gradient increase; the actual concentration of Cd(Ⅱ) is 2mg / L~120mg / L gradient increase), the initial pH value is adjusted to 7.0, and the temperature At 35°C, shake culture at 160r / min for 24h to carry out the shake flask experiment of Bacillus tropicalis resistant to heavy metals Cr(VI), Cd(II), Cu(II), and Pb(II), and monitor the OD in the sample solution 600nm value. Figure 3-4 It reflects the resistance of Bacillus tropicalis to different initial concentrations of Cr(Ⅵ), Cd(Ⅱ), Cu(Ⅱ), and Pb(Ⅱ). It can be seen that the maximum tolerance concentrations of Bacillus tropicalis to Cr(Ⅵ), Cd(Ⅱ), Cu(Ⅱ), and Pb(Ⅱ) are 535mg / L, 120mg / L, 230mg / L, and 200mg / L, resp...

Embodiment 3

[0062] Study on the degradation ability of strains of waste engine oil under single metal stress.

[0063] In order to study the degradation ability of Bacillus tropicalis under single metal stress, the cell fermentation liquid (OD 600nm =1.3±0.02) Inoculate 60mL inorganic salt medium containing different concentrations of heavy metals (Pb(II), Cu(II), Cr(VI), Cd(II) at 10mg / L, 20mg / L, 30mg / L, 40mg / L, 50mg / L, 60mg / L, 70mg / L, 80mg / L, 90mg / L), the initial waste engine oil concentration is 500mg / L, and the initial pH value is adjusted to 7.0, The temperature is 35°C, shake culture at 160r / min for 120h to carry out the shake flask experiment of Bacillus tropicalis degrading waste engine oil, monitor the concentration of waste engine oil in the sample liquid, Figure 5 It reflects the degradation of waste engine oil by Bacillus tropicalis under the stress of different concentrations and different types of heavy metals. It can be seen that when the concentration of Pb(II), Cu(II)...

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Abstract

Aiming at the defects in the prior art, the invention provides bacillus tropicalis capable of tolerating the toxicity of heavy metals and effectively degrading waste engine oil and an application of the bacillus tropicalis. The bacillus tropicalis is preserved in Guangdong Microbial Culture Collection Center on May 24, 2021, and the preservation number is GDMCC No.61680. The strain can tolerate the toxicity of high-concentration heavy metals, and meanwhile, the strain grows by taking waste engine oil as a carbon source and degrades the waste engine oil. The strain can be used for degrading waste engine oil with heavy metals in soil.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a bacillus tropicalis capable of effectively degrading waste engine oil resistant to heavy metals and its application. Background technique [0002] The traditional oil pollution problem is still widespread and increasing. In addition, driven by policies, the development of polluted land left by pollution-producing enterprises represented by oil companies has become a new problem. It is mainly reflected in two aspects: first, with the construction of my country's industrial parks, there have been many incidents of enterprises relocating and "entering" the parks, and the land at the old sites has generally suffered from relatively serious oil pollution; second, under the country's strict environmental protection policies Driven and promoted by the internal technology upgrading of the industry, the closure of some "soil refining" and low-capacity enterprises has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/07
CPCB09C1/10
Inventor 姜岩夏如馨彭蕾
Owner CHONGQING TECH & BUSINESS UNIV
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