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Diaphragma juglandis acidic polysaccharose and preparation and application thereof

A technology of acidic polysaccharides and distracted wood, applied in the field of distracted wood acidic polysaccharides and its preparation and application, can solve the problem of repeated and small-scale test stages of retention levels, failure of large-scale application of distracted woody polysaccharides, unseen analysis Achieving the effects of low cost, easy control, and promotion

Active Publication Date: 2015-06-24
FENGQING COUNTY YUZEGU WALNUT PROFESSIONAL COOP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there are few in-depth studies on the preparation and application of distracted xylopolysaccharides.
The polysaccharide PDJL-A3 extracted and separated from Xinjiang walnut septum by Gao Li et al. has a certain ability to scavenge superoxide and hydroxyl radicals, but the activity is not obvious (Gao Li, Xinjiang walnut septum polysaccharide extraction, purification and antioxidant activity research , Master Degree Thesis of Xinjiang University, 2010)
[0004] Most of the above-mentioned reports are about the preparation method and biological activity research of the water extract of fenugreek. Large-scale application in China, and no distracting xylopolysaccharide products

Method used

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  • Diaphragma juglandis acidic polysaccharose and preparation and application thereof
  • Diaphragma juglandis acidic polysaccharose and preparation and application thereof
  • Diaphragma juglandis acidic polysaccharose and preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0058] (1) Water extraction: Clean the heartwood produced in Yunnan with tap water, dry it in an oven at 40°C until it reaches constant weight, crush it to obtain the heartwood powder, add distilled water, add 30mL of distilled water to each gram of heartwood powder, and mix well Then extract in a water bath at a constant temperature at 95°C for 3 hours, centrifuge at 3500rpm at 4°C for 20 minutes, and take the supernatant; the precipitate obtained by centrifugation is repeated twice according to the water extraction method described above, and the supernatant is combined to obtain the aqueous extract of xylopolysaccharide ;

[0059] (2) Alcohol precipitation: in the aqueous extract of xylopolysaccharide in step (1), add the ethanol aqueous solution that accounts for 4 times of the volume of aqueous extract of xylopolysaccharide in the center of focus, and the volume percent concentration is 90%, stir and mix , placed in a refrigerator at 4°C for precipitation overnight, then ...

Embodiment 2

[0064] (1) Water extraction: clean the fenugreek produced in Yunnan with tap water, dry it in an oven at 40°C to constant weight, crush it to obtain fenugreek powder, add distilled water, add 20mL distilled water per gram of fenugreek powder, and mix well Afterwards, extract in a water bath at a constant temperature at 90°C for 2 hours, centrifuge at 3,000 rpm at 4°C for 15 minutes, and take the supernatant; the precipitate obtained by centrifugation is repeated twice according to the water extraction method described above, and the supernatant is combined to obtain the aqueous extract of xylopolysaccharide ;

[0065] (2) Alcohol precipitation: in the aqueous extract of xylopolysaccharide in the step (1), add the ethanol aqueous solution that accounts for 4 times of the volume of the aqueous extract of xylopolysaccharide in the center of focus, and the volume percentage concentration is 95%, stir and mix , placed in a refrigerator at 4°C for precipitation overnight, then centrif...

Embodiment 3

[0070] (1) Water extraction: Clean the heartwood produced in Yunnan with tap water, dry it in an oven at 40°C to constant weight, crush it to obtain the heartwood powder, add distilled water, add 40mL of distilled water to each gram of heartwood powder, and mix well Then extract in a water bath at a constant temperature at 100°C for 4 hours, centrifuge at 4000rpm at 4°C for 30 minutes, and take the supernatant; the precipitate obtained by centrifugation is repeated twice according to the water extraction method described above, and the supernatant is combined to obtain the aqueous extract of xylopolysaccharide ;

[0071] (2) Alcohol precipitation: in the aqueous extract of xylopolysaccharide in the step (1), add the ethanol aqueous solution that accounts for 5 times of the volume of the aqueous extract of xylopolysaccharide in the center of focus, and the volume percentage concentration is 96%, stir and mix , placed in a refrigerator at 4°C for precipitation overnight, then ce...

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Abstract

The invention discloses a diaphragma juglandis acidic polysaccharose and a preparation and an application thereof. The diaphragma juglandis acidic polysaccharose is prepared from polysaccharide with the weight percent being 99% or above, wherein the polysaccharide comprises galacturonic acid, glucose, galactose and arabinose at the mass ratio of (66.30-72.50) to (13.50-18.60) to (6.20-11.40) to (2.80-4.40); the preparation method of the diaphragma juglandis acidic polysaccharose comprises the following steps: extracting diaphragma juglandis powder crude polysaccharide by water extraction and alcohol precipitation, removing protein by employing an enzyme-Sevage combined technique, dialyzing and purifying by anion exchange chromatography and gel filtration chromatography; and carrying out vacuum freeze-drying, separating and purifying to obtain the diaphragma juglandis acidic polysaccharose. The purified polysaccharide is subjected to component analysis, structural identification and immune function research, so that the diaphragma juglandis acidic polysaccharose has relatively high anti-oxidation activity, can be used as an antioxidant or applied to preparation of the antioxidant, and can be widely applied to cosmetics, food supplements, animal feed additives, medicines and the like.

Description

technical field [0001] The invention relates to the field of natural plant polysaccharides, in particular to a kind of acidic polysaccharide from woody wood and its preparation and application, and belongs to the field of macromolecule and molecular biology. Background technique [0002] Fenxin wood is derived from the dry woody septum of the Juglans regia L. core of the Juglans plant. Astringent essence, clinical indications for seminal emission, gonorrhea, hematuria, leukorrhea, diarrhea, etc. In recent years, many compounds such as flavonoids, polysaccharides, saponins, quinones, and phenolic acids have been isolated and obtained from fenugreek, and the contents of flavonoids, tannins, and sugars have been determined. Pharmacological activity studies have shown that in addition to antibacterial and anti-oxidative effects, Fenxinmu has a significant effect on the treatment of kidney deficiency, spermatorrhea and enuresis. [0003] In recent years, there have been many re...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K8/73A61K31/715A61Q19/08A61P17/18A61P39/06A23L1/09A23K1/16
Inventor 贺亮李杰君李文魁李晓君
Owner FENGQING COUNTY YUZEGU WALNUT PROFESSIONAL COOP
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