Method for improving tobacco extractive incense by multiple biological enzymes

A tobacco extract, biological enzyme technology, applied in the directions of tobacco, tobacco preparation, application, etc., can solve the problems of less varieties of tobacco extract, hindering the development of the tobacco industry, etc.

Active Publication Date: 2006-02-15
YINGTAN HUABAO FLAVORS & FRAGRANCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] But, up to now, domestic tobacco extract kind is still relatively few, and quality still needs to be improved further, has hindered the further development of tobacco industry to a certain extent, for this industry sector

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029]Weigh 10 grams of flue-cured tobacco extract in a bioreactor, add 50 grams of deionized water, stir at a speed of 200 rpm, and stir for 20 minutes. Heat up to 85°C at a rate of 8°C / min and keep for 10 minutes for sterilization. After sterilization, cool the system quickly with water, and stop cooling when the temperature drops to 50°C. Under stirring, add 0.2% (W / W) cellulase, hemicellulase and pectinase respectively, the speed of stirring is controlled at 50 rpm, and the pH of the system is adjusted with 0.1N hydrochloric acid or sodium hydroxide to 5, enzymatic hydrolysis for 2 hours. After the enzymolysis of cellulase, hemicellulase and pectinase is completed, add 0.3% (W / W) β-glucosidase, the temperature of enzymolysis is still controlled at 50°C, the pH of the system is 5, and the enzyme The solution time is 2 hours. After the enzymatic hydrolysis is completed, heat the enzymatic hydrolysis system to inactivate the enzyme. The temperature for inactivating the enz...

Embodiment 2

[0031] Weigh 10 grams of flue-cured tobacco powder extract in a bioreactor, add 100 grams of deionized water, and stir at a speed of 200 rpm for 15 minutes. Heat up to 90°C at a rate of 10°C / min and keep for 5 minutes for sterilization. After sterilization, cool the system quickly with water, and stop cooling when the temperature drops to 55°C. Under stirring, add 0.1% (W / W) cellulase, hemicellulase and pectinase respectively, the speed of stirring is controlled at 50 rpm, and the pH of the system is adjusted with 0.1N hydrochloric acid or sodium hydroxide To 5.5, enzymatic hydrolysis for 90 minutes. After the enzymolysis of cellulase, hemicellulase and pectinase is completed, add 0.2% (W / W) β-glucosidase, the temperature of enzymolysis is controlled at 50°C, the pH of the system is 5.5, and the enzymolysis The time is 2 hours. After the enzymatic hydrolysis is completed, heat the enzymatic hydrolysis system to inactivate the enzyme. The temperature for inactivating the enz...

Embodiment 3

[0033] Weigh 10 grams of oriental tobacco extract into a bioreactor, add 80 grams of deionized water, and stir at a speed of 200 rpm for 20 minutes. Heat up to 75°C at a rate of 8°C / min and keep for 30 minutes for sterilization. After sterilization, cool the system quickly with water, and stop cooling when the temperature drops to 45°C. Under the condition of stirring, add 0.3% (W / W) compound enzyme of cellulase, hemicellulase and pectinase, the speed of stirring is controlled at 50 rpm, adjust the system with 0.1N hydrochloric acid or sodium hydroxide pH to 6, enzymatic hydrolysis for 2 hours. After the enzymolysis of cellulase, hemicellulase and pectinase is completed, add 0.1% (W / W) β-glucosidase, the temperature of enzymolysis is controlled at 50°C, the pH of the system is adjusted to 5, and the enzyme The solution time is 90 minutes. After the enzymatic hydrolysis is completed, heat the enzymatic hydrolysis system to inactivate the enzyme. The temperature for inactivat...

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PUM

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Abstract

Disclosed is a method for improving tobacco extractive incense by multiple biological enzymes, which comprises charging pectolase, cellulose, hemicellulase and beta-glucosidase for enzymolysis, thus producing many kinds of scent generation constituents originally non-existent in the tobacco extract, as well as a plurality of micromolecular carbohydrates and beta-galacturonic acid.

Description

technical field [0001] The invention relates to a method for preparing spices, in particular to a production method for enhancing and improving the aroma of tobacco extract by treating tobacco extract with biological enzymes. Background technique [0002] Tobacco extract is a dark brown viscous paste with a characteristic aroma of tobacco. According to the variety of tobacco leaves, tobacco extracts can generally be divided into three categories: flue-cured tobacco extracts, burley tobacco extracts and oriental tobacco extracts. Common tobacco extracts include Yunyan extract, Yuyan extract, oriental tobacco extract, Fenghuang tobacco extract and Burley tobacco extract. Tobacco extract can be used in tobacco essence to enhance the natural aroma, roundness and smoke that complement the inherent characteristics of tobacco, and to cover up the endemic flavor of tobacco leaves in a single region. The dosage in flue-cured tobacco flavor is 3%-5%, and the dosage in mixed flavor: ...

Claims

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Application Information

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IPC IPC(8): A24B3/12C11B9/00
Inventor 罗昌荣穆海菠翁昔阳赵慧黄汉荣
Owner YINGTAN HUABAO FLAVORS & FRAGRANCES
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