Gentian acidity uniform polysaccharide and purification method and application thereof

A purification method and technology of gentian are applied in the field of gentian acidic homogeneous polysaccharide and its purification, which can solve the problems of few reports on the separation and purification of gentian polysaccharide, the determination of molecular weight of components and the unreported anticoagulant activity, etc. The effect of developing application prospects, simple and feasible operation, and avoiding potential safety hazards

Active Publication Date: 2015-09-30
ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on gentian polysaccharide mainly focuses on the extraction and other biological activities, but there are few reports on the separation and p...

Method used

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  • Gentian acidity uniform polysaccharide and purification method and application thereof
  • Gentian acidity uniform polysaccharide and purification method and application thereof
  • Gentian acidity uniform polysaccharide and purification method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035]A gentian acidic homogeneous polysaccharide, comprising the following monosaccharides and their molar percentages: 1.1% fucose, 11.5% rhamnose, 22.5% arabinose, 17.9% galactose, 3.3% glucose, xylose 3.1%, mannose 0.7%, galacturonic acid 39.9%. The specific preparation method is as follows:

[0036] polysaccharide extraction

[0037] After cleaning the root of gentian, dry it in an oven at 60°C, then pulverize it with a pulverizer, and pass through an 80-mesh sieve to obtain gentian powder. The mass-volume ratio of gentian powder and distilled water to the feed liquid is 1:25g / mL was leached at 90°C for 3 hours, and after suction filtration, the filtrate was obtained, and the filtrate was vacuum concentrated to 20% of the original volume at 55°C (the purpose was to reduce the amount of ethanol and subsequent centrifugation times) to obtain a concentrate , slowly add 4 times the volume of absolute ethanol and keep stirring, and place it in a refrigerator at 4°C for ov...

Embodiment 2

[0062] Preparation of anticoagulant health product—gentian drink

[0063] In parts by weight, the ratio of water to gentian coarse powder is 1 to 15:100, the extraction temperature is 75 to 95°C, and the time is 5 to 30 minutes. ℃, placed for 24h to clarify, and 0.5μm fine filtration to obtain clarified gentian juice. 0.04-0.2% gentian acid homogeneous polysaccharide GSP-3, 3-6% sucrose, 0.03-0.07% ascorbic acid and other auxiliary materials are firstly dissolved in some warm water and filtered. Mix it with the finely filtered gentian juice, sterilize at 135°C for 5-7s, and bottle it to obtain an anticoagulant health-care gentian drink.

Embodiment 3

[0065] Preparation of anticoagulant drugs

[0066] Get 4g gentian acid homogeneous polysaccharide GSP-3, 500g microcrystalline cellulose, 450g dextrin, 75g low-substituted hypromellose, 75% ethanol 75mL, magnesium stearate 5g, press 2000 by wet granulation tableting method piece.

[0067] Granules: take 4g gentian acid homogeneous polysaccharide GSP-3, 1400g dextrin, 100g powdered sugar, 50g tartaric acid, 50% ethanol in appropriate amount, use wet granulation to sieve, mix soft materials, extrude into granules, and dry at 60°C , packaged after whole grains, 2-5g per bag.

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Abstract

The invention discloses a gentian acidity uniform polysaccharide and a purification method and application thereof and belongs to the field of gentian acidity uniform polysaccharide purification. The gentian acidity uniform polysaccharide comprises the following components monosaccharide including, by mole percentage content, 1-1.5% of fucose, 11-12% of rhamnose, 22-23% of arabinose, 17-18% of galactose, 3-3.5% of glucose, 3-3.5% of xylose, 0.5-1% of mannose and 39-40% of galacturonic acid. According to the gentian acidity uniform polysaccharide and the purification method and the application thereof, further separation and purification of the gentian polysaccharide are achieved, the gentian acidity uniform polysaccharide with the weight-average molecular weight of 5.8*104 Da is obtained, the anticoagulant activity of the gentian acidity uniform polysaccharide is high, the preparation method is simple, the hidden danger existing in the residual chemical reagent is avoided, and the gentian acidity uniform polysaccharide has good development application prospect.

Description

technical field [0001] The invention belongs to the technical field of gentian polysaccharide purification, and in particular relates to a gentian acidic homogeneous polysaccharide and its purification method and application. Background technique [0002] With the improvement of people's living standards and changes in diet structure, cardiovascular and cerebrovascular diseases have become the main cause of death after cancer. Most patients with thrombosis require anticoagulant therapy during surgery. Heparin is the most common anticoagulant drug used in clinical treatment, and plays a very important role in the treatment of the above diseases. However, most of the production of heparin comes from animal organs, which may carry pathogenic microorganisms, animal allergens and pyrogens, and may cause harm to human health. In addition, the use of heparin is often accompanied by some side effects such as: easy to cause bleeding, hyperkalemia, heparin-induced thrombocytopenia a...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P7/02A23L2/52A23L1/09
Inventor 蔡为荣许惠玲
Owner ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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