164results about How to "Realize separation and purification" patented technology

The invention relates to the red beam luminescent dye which the receptor group is the 1, 3-indandione and the derivant 4H-pyrones. So the quenching effect of the new dyestuff has decreased efficiently and it has the saturated colour-purity. It can keep high efficient and the brightness in the high doping content. It is used for the organic photoconductor, the organic nonlinear optical material and the luminescent material of the electroluminescent cell. The emission band spectrum is in the red region of the visible spectrum and it has the high fluorescence quantum efficiency.

The invention provides a method for extracting high-purity sodium sulfate and sodium chloride products from high-salt content wastewater in the coal chemical industry. The method comprises the following steps: firstly, pretreating the high-salt content wastewater in the coal chemical industry to reduce the hardness thereof and primarily concentrate the wastewater till the TDS content is about 30000mg/L, and primarily separating sodium sulfate and sodium chloride from the high-salt content wastewater in the coal chemical industry by a nanofiltration process to obtain nanofiltration concentrated water with the sodium sulfate as a main component and a nanofiltration produced water with the sodium chloride as a main component; secondly, removing a part of organic matters from the nanofiltration concentrated water to reduce the COD by an electrolytic oxidizing technology, and performing a hot crystallization technology and a freezing crystallizing technology to obtain a pure sodium sulfate product and a small amount of sodium chloride product; treating the nanofiltration produced water by a membrane concentration technology and a hot crystallizing technology to obtain a pure sodium chloride product. In the whole technical process, on the basis of achieving zero discharge of the wastewater, reuse of all water and maximized recycle of the salts in the wastewater are achieved, so that the economic efficiency is improved and the environment pollution is avoided.

The invention discloses a method for coproducing collagen activity peptides and flavoured base materials with chicken bones. Through a two-step hot pressing and extracting method, and through slight pressure relief and pressure supplementation treatment, the yield of extracting the collagen activity peptides from the chicken bones is substantially increased. Through high-pressure homogenizing of osseins in extracting solutions before a step-by-step enzymolysis reaction of the osseins, the degree of hydrolysis of enzymolysis solutions is increased. In addition, two-step ultra filtration is performed on the enzymolysis solutions, and separation and purification of different products in the enzymolysis solutions are realized. Follow-up processing is performed aiming at the products of each molecule segment, different products of bone polysaccharide containing chondroitin sulfate, macromolecule polypeptide, micromolecule polypeptide and the like are obtained, making full use of ossein enzymolysis solutions is realized, and especially a Maillard reaction is performed on the macromolecule polypeptide, xylose, cysteine and thiamin to obtain the flavoured base material with a rich flavour.

The invention discloses a method for producing alpha-oxoglutarate under catalysis of L-glutamate oxidase. The method comprises the following steps: performing optimal design and synthesis on codon of an L-glutamate oxidase (L-GOX) gene; constructing a fusion expression vector pET-28a-LGOX-ELP of the L-GOX and Elastin-Like Proteins (ELP), transferring the vector into escherichia coli Rosetta (DE3), selecting a converter for cultivation, adding an inducer for expression, and obtaining recombined L-glutamate oxidase fusion protein crude enzyme fluid; performing purification by an ITC method, thus obtaining recombined L-glutamate oxidase fusion proteins, performing enzyme digestion on the recombined L-glutamate oxidase fusion proteins by protease, and obtaining mature L-glutamate oxidase; producing oxoglutarate under the catalysis of the mature L-glutamate oxidase. The method is simple, quick and efficient, can be used for large-scale separation and purification of L-glutamate oxidase, and is higher in actual application value in the enzyme production of oxoglutarate.

The invention relates to a method for preparing a material containing amorphous iron oxide hydroxide and two methods for regenerating the same after being used as desulfurizer. The content of the amorphous iron oxide hydroxide in the material prepared by the invention is as high as 65% to 100%, so the sulfur capacity of the material is high; and the desulfurizer in the prior art is non-regenerative or costly for regeneration, so that the desulfurizer in a large amount cannot but be dumped as waste liquid in landfills, while the amorphous iron oxide hydroxide in the material of the invention can be repeatedly regenerated and reused after the material is used as the desulfurizer, thereby solving the problems of the waste of effective resources in the original desulfurizer and the severe environmental pollution.

The invention provides a novel process for preparing high-purity monascus pigment. The process comprises the following steps of: crushing red yeast rice as a raw material; and separating and purifying to obtain 6 kinds of monascus pigment components by adopting a composite technology of extraction liquid extraction and high-speed countercurrent chromatography separation and crystallization, wherein the six monascus pigment components comprise rubropunctamine, monascorubramine, monascin, ankaflavin, rubropunctatin and monascorubin, and the purities of all the pigments are higher than 95 percent and can be used for preparing foods, cosmetic colorants and health products. The invention adopts easily-recovered organic solvent for separation to save the consumption of separation materials andovercome the problems of small treatment amount and inefficient single pigment component separation and purification of a TLC (Thin Layer Chromatography), an HPLC (High Performance Liquid Chromatography), a resin method and a silica gel column chromatography. The invention adopts the composite technology of the extraction liquid extraction and the high-speed countercurrent chromatography separation and the crystallization to prepare six kinds of high-purity monascus pigment crystals and has the advantages of simple steps and high yield.

The invention discloses an arsenic fixing method for preparing scorodite through stabilization treatment of arsenic-alkali residue. The arsenic fixing method comprises the following steps: (1) the arsenic-alkali residue is subjected to oxidation leaching, filtering is conducted to obtain leachate containing sodium carbonate and sodium arsenate, and sodium antimonite precipitation, after the leachate is concentrated, CO2 is introduced for dealkalization, and filtering is conducted to obtain dealkalized leachate and sodium bicarbonate crystals; (2) acid is added into the dealkalized leachate obtained in the step (1) to control the pH of the dealkalized leachate to be 1.0-2.5, and an arsenic-rich solution is obtained; and (3) a mixed solution of ferrous salt and H2O2 is added into the arsenic-rich solution obtained in the step (2) according to the iron-arsenic molar ratio of 1.0-3.0, the pH of the mixture is controlled to be 1.2-2.0, the mixture reacts at 75-95 DEG C, and scorodite crystals are obtained. The arsenic-alkali residue is treated to obtain the scorodite crystals with the double-cone octahedron shape and uniform particles, the arsenic leaching concentration is lower than the concentration stipulated by the GB5085.3-2007 'hazardous waste identification standard-leaching toxicity identification', and the scorodite crystals can be safely stored for a long term.

The invention relates to a column-switching circulating volume exclusion chromatography system for biomacromolecule separation and purification, which comprises a liquid chromatography pump, a sampling valve, a three-way or four-way connector, a switching valve A, a switching valve B, a volume exclusion column A, a volume exclusion column B and a detector. The pump, the sampling valve, the volume exclusion column A, the switching valve A, the volume exclusion column B, the detector, the switching valve B and the three-way or four-way connector are connected with one another in turn through pipelines, the number of the biomacromolecules getting into a circulation and separation system can be controlled by adjusting the switching frequency or mode of the valve A, and the separation and the collection of the biomacromolecule components can be controlled by adjusting the switching of the valve B. Compared with the conventional volume exclusion chromatography, the system has the characteristics of strong separating power, high resolution and the like, and can be applied to the separation and the purification of biomacromolecule samples. Simultaneously, the system also has good utility value in constructing a continuous biomacromolecule multi-dimensional separation platform.

The invention relates to a system and method for comprehensive treatment of garbage and carbide slag. The system comprises a carbide slag treatment unit, a mixed forming unit, a pellet pyrolysis unitand a high-temperature roasting unit; the carbide slag treatment unit comprises a carbide slag inlet, a raw coke-oven gas inlet, a syngas outlet and a dried carbide slag outlet; the mixed forming unitcomprises a dried carbide slag inlet, a garbage inlet and a mixed pellet outlet; the pellet pyrolysis unit comprises a mixed pellet inlet, a flue gas outlet, a raw coke-oven gas outlet and a high-temperature pyrolysis pellet outlet; the high-temperature roasting unit comprises a high-temperature pyrolysis pellet inlet, a CO2-rich gas outlet and a roasted ceramsite outlet. The system and method ofthe comprehensive treatment of the garbage and carbide slag disclosed by the invention use an anaerobic pyrolysis furnace to carry out co-pyrolysis on the garbage and the carbide slag, and an obtained product is in direct contact with the carbide slag, so that the high-quality syngas is obtained while the carbide slag is dried; furthermore, the solidification effect of calcium oxide in the carbide slag on garbage heavy metals is fully utilized to prepare safe and high-performance building ceramsite.

The invention provides a biological enzyme purification separating device. The biological enzyme purification separating device comprises a raw material storage tank, a Graef pump, a micro-filter tube, a rejection liquid collection pump, a wash tank, a flow adjusting device, a valve, a pressure gage, a flow gauge, and a material tube. The biological enzyme purification separating device can achieve continuous filtering and separating of high turbidity liquid under the fully sealed condition and maintaining of high activity of zymin. Due to the fact that the micro-filter tube, with the pore diameter of 0.1-0.2 microns and high-strength, acid-base resistance and corrosion resistance, of a hollow fiber organic membrane or ceramic or acstainless steel component is adopted, the biological enzyme purification separating device can efficiently conduct edulcoration and degerming and achieve separation and purification effect, at the same time, cannot damage an original ingredient, an original structure, an original special flavor, and original energy activity, improves sanitary quality of products. The biological enzyme purification separating device is widely applied for filtering and separation of variousbiological enzymes with strict demands for microorganismsanitation and enzyme activity and at the same time is further suitable for filtering and separation of certain simple liquid state drinks and condiments.

The invention provides a separation and recycle system for water-containing organic waste liquid and organic solvents as well as a recycle method, wherein the separation and recycle system consists ofa distillation system and a pervaporation membrane separation system through process pipeline connection. A packed tower is adopted as a rectifying tower, and the material inlet of the packed tower is arranged in the center of a packed tower liquid distributor. A damping structure is arranged in the pervaporation membrane unit of the pervaporation membrane separation system. The separation and recycle system provided by the invention is suitable for online dehydration and recycle of multiple kinds of water-containing and two or more than two azeotropic or non-azeotropic organic solvents as well as for separation and reuse of the organic solvents.

The invention discloses a method for carrying out extraction, inspection and content determination on 5-hydroxytryptamine in sea buckthorn and relates to the field of extraction, inspection and content determination of indole derivatives in the sea buckthorn. Sea buckthorn fruit peels and fruit branches are taken as a raw material; the raw material is smashed and screened; absolute ethyl alcohol is added; ultrasonic microwave extraction is carried out; centrifugal filtration is carried out; the raw material is concentrated to 1/4; methanol is added for dissolution; normal hexane is added, andmixing, extraction and concentration are carried out; ethyl acetate is added for dissolution; after sufficient dissolution is carried out, ultrapure water is added; liquid separation is carried out; ethyl acetate phase is obtained and is concentrated to dry; little absolute ethyl alcohol is added for dissolution; volume is fixed at 50mL; content is determinated through TLC reflection scanning; molecular weight information of samples is determinated through utilization of a mass spectrometer; functional groups are detected through infrared; optical rotation and specific rotation are determinated through utilization of a polarimeter; evaporation and crystallization are carried out; and a melting point is determinated. According to the method provided by the invention, time and labor are reduced; a separation effect is relatively good; purity is high; a result is accurate; and the method for carrying out the extraction, inspection and content determination is simple in operation and highin efficiency.

The invention relates to a recombinant beta-glucosidase and an expression and purification method and the immobilization application thereof, and belongs to the technical field of biological enzyme engineering. The recombinant beta-glucosidase is recombinant beta-glucosidase Glu-linker-ELP-GB (GLEGB) obtained by linking binary labels ELP and GB with beta-glucosidase (Glu) through a universal linking peptide linker, wherein the sequence of GB polypeptide is linked to the 3' end of an ELP gene. By virtue of a reversible phase change property of an ELP label on recombinant enzyme, separation andpurification of the enzyme can be achieved; the recombinant enzyme containing the ELP label can be precipitated only by adding a certain amount of (HN4)2SO4 for incubation for a period of time; by virtue of a temperature response behavior of the ELP label, one-step rapid separation and purification of a target enzyme molecule is achieved; the enzyme purifying effect of the expression and purification method is superior to that of an ammonium sulfate precipitation method, and the purification cost of the expression and purification method is much lower than that of a chromatography method.

The invention provides a method for removing calcium and magnesium from a bioleaching solution of nickel cobalt by a synergetic extraction method. The method comprises the following steps: homogeneous saponification is separately carried out for a nickel cobalt extractant Versatic10 and a synergic extractant Cynaex301 by using a NaOH solution, and saponification rate is 40-60%; an organic phase is composed of a combined extractant and a diluent Mextral DT100; the combined extractant comprises 20-40V% of the synergic extractant Cynaex301 and 80-60V% of the extractant Versatic; the volume of the combined extractant is 10-30% of the total volume of the organic phase; when an initial pH value is 1.5-3.0, the organic phase and the bioleaching solution of nickel cobalt are mixed with shaking, the pH value is controlled at 3.0-4.0 for balancing extraction, and after standing, the organic phase and the aqueous phase are separated; sulfuric acid is added into the organic phase for back extraction of nickel cobalt, so that effective separation between nickel cobalt as well as calcium and magnesium is realized.

The present invention relates to gene engineering, and is one kind of expression vector of human hair papilla cell HSPC016 gene. The vector is plasmid pET-22b(+) containing the functional sequence of HSPC016 gene, and the HSPC016 coded sequence is located between Nde I and Xho I cleavage site of pET-22b(+) vector. The present invention also relates to one kind of gene engineering bacterium transformed with the said vector and provides process of preparing human hair papilla cell HSPC016 target protein. The HSPC016 target protein is expressed through inducing colibacillus pET-22b(+)/HSPC016/BL21 with IPTG. The present invention has the advantages of high target protein expressing amount, expression rate over 28 %, addition of 6xHis purification label for easy protein purification, high bioactivity of the target protein, etc.

The invention discloses a separation and purification device for C8-C20 n-alkane mixed fractions and a separation and purification process thereof, the separation and purification device combines a partition plate rectifying tower and a conventional rectifying tower, the traditional multi-tower series process is simplified, a side extraction stream is added, and the separation of a target productis realized. The separation and purification are carried out on the target product C8-C20 n-alkane mixed fraction; according to the method, the seven products, namely the lightest C8-C11 mixture, theheaviest C17-C20 mixture and the high-purity C12, C13, C14, C15 and C16 single-component products, are obtained, so that the traditional process flow is greatly simplified, the energy consumption of the whole process is remarkably reduced, and the method has remarkable practicability and economical efficiency.