Method of separating water soluble monascus pigment by the use of macroreticular resin and application thereof

Abstract

The invention discloses a method of separating water soluble monascus pigment by the use of macroreticular resin and application thereof. The method comprises the steps of conducting solid-liquid separation on fermented monascus fungi fermentation primary liquid in a liquid fermentation medium, taking liquid to obtain extracellular water soluble monascus pigment fermentation solution, then adding the macroreticular resin after pretreatment to conduct absorbing, then using ethyl alcohol to conduct elution, removing eluent from obtained eluant and conducting frozen vacuum drying on the eluant, and finally obtaining the water soluble monascus pigment. According to the method of separating the water soluble monascus pigment by the use of the macroreticular resin and application thereof, the problems of unstable water soluble monascus pigment and massive impurities in the fermentation solution are solved by utilizing the macroreticular resin to conduct separation and purification on the monascus pigment; meanwhile efficient separation of the extracellular water soluble monascus pigment is achieved by removing impurities of polysaccharide, inorganic salt and the like in the monascus pigment fermentation solution, and a good application prospect and market value are provided.

Description

technical field [0001] The invention relates to a method for separating monascus pigment, in particular to a method for separating water-soluble monascus pigment by using a macroporous adsorption resin and its application. Background technique [0002] Monascus pigment is a natural pigment produced by the fermentation of Monascus. The pigments extracted from the fermented red yeast rice by Monascus are mainly intracellular pigments. These pigments are easily soluble in polar organic solutions such as ethanol and acetone. , are non-water-soluble pigments, subject to certain restrictions in food applications. With the in-depth study of monascus pigment, the monascus pigment produced by liquid fermentation has good water solubility in recent years. Water-soluble Monascus pigment has good thermal stability, light resistance, oxidant resistance and reducing agent resistance, and its development and research have broad prospects and significant economic benefits. However, there ...

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Problems solved by technology

If it is reported that ion exchange resins (D380, D301R, 110H, etc.) are used to separate monascus pigments, there are problems such as difficulty in elution or low elution rate (Lian Xijun, etc., Food Industry Science and Technology, 2003, 24 (8): 81- 83); It has been reported that anion exchange resin D301 is used to separate water-soluble monascus pigments to obtain very high-purity monascus red pigments, but there is a problem that the pigments cannot be fully recovered (Xiong Hejian et al., Southwest University Journal of Natural Science Edition, 2009,31(9 ):20-24); It has been reported that macroporous adsorption resins (X-5, AB-8, D 14 、D 16 etc.) to separate the red yeast pigment, there are problems such as low recovery rate (Tong Qunyi, Sichuan Institute of Light Chemical Industry, 1999,12 (4): 9-12); there are reports to process the red yeast pigment waste liquid with macroporous resin NKA etc. There is the problem of low adsorption rate and elution rate (Rong Yanyun et al., China Food Additives, 2012, 01:154-157)

[0003] Usually, soluble proteins, peptides, and amino acids are added to the culture medium to promote the production of water-soluble red yeast pigments. Hassan Hajjaj et al. The ratio of carbon and nitrogen sources and the fermentation conditions increase the extracellular monascus red pigment, but make the composition of the fermentation broth more complex. The red, orange and yellow pigments combine with proteins, polypeptides, amino acids, glucosamine and other substances to form a variety of derivatives

In addition, Chen gong et al. (BMC Biotechnology, 2015, 15(1): 72-80) carried out high-density fermentation by adding a single carbon source (glucose), which greatly increased the extracellular yellow pigment of Monascus liquid fermentation, but the final red The residual sugar in the koji fermentation broth is higher than 25g / L, which increases the difficulty of the separation and purification of the water-soluble red yeast pigment products in the later stage. Therefore, a method for efficiently separating the water-soluble red yeast pigment is urgently needed