69 results about "Monascus ruber" patented technology

This invention relates to a method for producing orange coloring matters by fermenting monacolin in liquid state, which takes a monascus moldin 9903 as a starter to produce monascus colors with orange as the principle and increases its content and tint, determines a formula of the best fermentation and culture medium, in which, the color content of a fermented fluid with corn flour and (NH4)2SO4 as the carbon and nitrogen sources reaches to 121.7U/mL and its tint is 1.13. The method provides several refining methods incliding: spraying and drying the fermented fluid to get a powder orange product, which is a mixture of multiple color matters showing the orange color tint, the orange content of which reaches to 1030.75U/g and the tint reaches to 1.99, the fluid is extracted to be sprayed and dried to get the content to over 2000U/g and the tint to 2.45 or chromatographed with plate or post to get pure orange pin crystals.

The invention relates to a Monascus ruber for the high generation of lovastatin and gamma-aminobutyric acid and the low generation of a pigment. The Monascus ruber is named as C002, belongs to Monascus sp., has a preservation number of CGMCC NO.6860, is preserved at China General Microbiological Culture Collection Center in Institute of Microbiology, Chinese Academy of Sciences on Datun Road, Chaoyang District of Beijing on November 26, 2012. The Monascus ruber has the advantages of high generation of lovastatin and gamma-aminobutyric acid, no generation or extremely-low generation of citrinin, no bitterness, and low generation of a Monascus pigment. Strains of the Monascus ruber CGMCC NO.6860 can utilize a plurality of culture mediums comprising rice, coarse rice, corn, cassava and the like to produce functional red yeast rice; and the Monascus ruber is especially suitable for the exploitation of relevant functional beverage products because of the Monascus pigment low-generation characteristic.

The invention relates to a microorganism, in particular to a monascus strain capable of obtaining high content of gamma-reanal and Lovastatin, the strain is named Monascus ruber Danxi-1, which is preserved in SIPO designated China General Microbiological Culture Collection Center with a docket number of CGMCC No: 1405, the preservation date being June 10, 2005.

The invention provides chocolates containing tea polyphenols and a preparation method of the chocolates, and relates to the technical field of chocolates. The chocolates are prepared from the following components in parts by weight: 80-100 parts of cocoa powder, 10-15 parts of cane sugar, 10-20 parts of dried milk, 40-50 parts of plant liquid fat, 20-40 parts of cocoa butter, 0.4-0.6 part of resveratrol, 0.2-0.4 part of tannin, 4-6 parts of soybean lecithin, 12-20 parts of green tea powder, 0.02-0.04 part of monascus ruber, and 100-150 parts of distilled water. The preparation method comprises the following steps of mixing the raw materials; performing fine grinding, wherein after fine grinding, the particle diameter is 60[mu]m or below; performing refining; adjusting the temperature: adjusting the temperature to 20-30 DEG C, then adding the resveratrol, the tannin and the monascus ruber, and then performing sufficient stirring; and performing pouring, shaping, drying and packing. The chocolates are long in preservation time, high in beneficial components, and good in health-care effects, people can eat the chocolates for a long term without worrying about weight gain, and the chocolates are fine and smooth in mouth feel, good in taste, high in quality and free from blooming.

The invention belongs to the field of biotechnology microorganisms, and relates to a monascus purpureus M-24 bacterial strain for producing Monacolin K by virtue of liquid fermentation, as well as application and a preparation method of the bacterial strain. The Monascus purpureus M-24 bacterial strain is preserved at the China Center for Type Culture Collection; the preservation number is CCTCC NO:M2014414; the preservation date is September 14, 2014; the monascus purpureus M-24 bacterial strain has excellent characters of high production of Monacolin K, high growth speed, high sporulation quantity and the like. After liquid fermentation of a culture medium and optimization of fermentation conditions, the yield of the Monacolin K can reach 170mg/L to 210mg/L.

The invention discloses a production method of food safety monascorubin, which comprises the steps of monascus slant seed culture, liquid seed culture and fermentation culture, wherein a transforming agent is added in the fermentation culture process, and the transforming agent is one or more of carotene, vitamin C, fulvic acid and EDTA (Ethylene Diamine Tetraacetic Acid)-2Na. The citrinin content in the monascorubin prepared by the method is reduced greatly. The production method has the advantages that the production method is high in safety, easy and simple to operate and efficient.

The invention relates to a method for preparing a monascus pigment, in particular to a method for preparing a monascus red pigment by utilizing immobilized enzymes. The method comprises the steps as follows: taking a monascus liquid fermentation solution as a raw material, homogenizing, enzymatically hydrolyzing the immobilized enzymes, alkalifying and filtering to obtain a monascus red pigment solution; and carrying out acid deposition on the monascus red pigment solution, washing and neutralizing the deposits, dehydrating and drying to obtain the monascus red pigment. The method has the advantages as follows: on one hand, the fermentation solution is effectively dispersed, broken, emulsified and mixed by utilizing a colloid mill, so that the subsequent enzymatic hydrolysis efficiency is improved and the monascus red pigment in monascus is fully released; and on the other hand, an enzyme preparation can be recycled, the reaction conditions are moderate, the monascus red pigment is concentrated through the acid deposition method, the process is simple, the energy consumption is low, and the product is easy to separate. The production cost can be remarkably reduced through the monascus red pigment prepared with the method. The monascus red pigment has the maximum absorption wavelength of 499 nm, is good in water solubility and light, heat, acid, alkali and metal stabilities, and is favorable for the monascus pigment industry development.

The invention discloses a method for preparing open-loop lovastatin by using monascus purpureus, which is a biological conversion method for utilizing monascus to produce enzyme and hydrolyze closed-loop lovastatin into the open-loop lovastatin. The method comprises the following steps: selecting and culturing a monascus strain CGMCC NO.0272; putting the cultured monascus strain CGMCC NO.0272 intoa fermentation medium for fermentation; preparing crude enzyme solution by a salting out method; performing substrate preparation; performing enzymatic conversion reaction; and performing other steps. The method for preparing the open-loop lovastatin by using the monascus purpureus adopts microbial conversion means, and compared with chemical means, the microbial conversion means has the advantages of moderate condition, simple equipment, less public hazard, quick reaction rate and relatively environmental protection.

The invention discloses a monascus phy gene and application thereof in increasing the yield of yellow pigment. The monascus phy gene disclosed by the invention plays an important role in increasing the yield of the yellow pigment fermented by monascus, the nucleotide sequence of the monascus phy gene is shown as SEQ ID NO.1, and the encoded amino acid sequence of the monascus phy gene is shown asSEQ ID NO. 2. The application specifically comprises the following step: constructing a displacement type gene targeting vector to knock out the phy gene. The yield of the obtained phy gene knockout strain red kojic rice solid-state fermentation monascin is 3.66 times higher than the yield of an original strain, and the yield of ankaflavin is increased by 81.5%.

The invention provides a method for increasing yield of monascus pigment. According to the technical scheme, starting from a plant dual-element plasmid pCambia0380 vector, a dual-element plasmid vector pHph0380 suitable for knockout of flamentous fungus genes is modified and constructed. On the basis, upstream and downstream homologous arm fragments of glucose transporter gltp1 genes are cloned from monascus ruber CICC41233 and connected with the plasmid vector pHph0380, the fragments are transformed into parent monascus ruber through agrobacterium tumefaciens EHA105 mediation, and a recombinant strain is constructed. Monascus pigment fermentation results prove that the recombinant strain constructed with the method has monascus pigment accumulation time advanced significantly, the alcohol-soluble pigment yield is obviously increased, and when fermentation is performed for 144 h, the alcohol-soluble pigment yield is 74% higher than that of parent strains. The fermentation time of monascus for producing monascus pigment is advanced by use of a gene engineering means, the utilization rate of starch is increased, and the yield of the monascus pigment is increased.

The invention provides a method for improving extracellular pigment of Monascus. The method comprises knocking out gene encoding ERG4/ERG24 family protein on ergosterol synthesis pathway in Monascus.ERG4/ERG24 gene encode in Monascus are knocked out by an agrobacterium-mediated transformation technology. As that plant binary plasmid pCAMBIA1300 carry is used, the recombinant plasmid is mediate byAgrobacterium tumefaciens EHA105 to be transformed into Monascus, the production level of extracellular pigment is remarkably improved, and the result shows that the recombinant strain has good passage stability. A liquid fermentation result show that that extracellular monascus pigment of the parent strain is 4.92 U/mL, the extracellular monascus pigment of the recombinant strain constructed bythe invention is 6.52 U/mL, and the production amount of the extracellular pigment is increase by 32.52%, which shows that the production amount of the extracellular pigment of the monascus can be remarkably increased by the method of the invention.

The invention belongs to the field of biological feed, and relates to a monascus strain, biological feed fermented by the monascus strain and application of the red yeast rice biological feed in aquaculture. The monascus strain can generate multiple kinds of functional metabolites of monascus pigment, protease, amylase, lipase, ergosterol, statins, gamma-aminobutyric acid and the like, and fermentation products contain small peptides. By applying the biological feed to aquaculture, the survival rate and immune functions of aquatic animals can be significantly improved, and the growth performance is improved.

The invention relates to a fruit-vegetable-cereal fermentation beverage and a brewing process thereof. The brewing process comprises: fermenting cereal by using Monascus ruber to obtain Monascus ruberfermented cereal, mixing water and Monascus ruber fermented cereal, and adding the mixture into a cooking container; washing fruits and vegetables, removing mould rot parts, carrying out air drying,slicing, adding into the cooking container, and cooking the fruit and vegetable slices and the Monascus ruber fermented cereal soaked with the water to achieve a cooked state; and adding a small amount of white koji, spreading the cooked fruit and vegetable and the cooked Monascus ruber fermented cereal, cooling, placing into a storage container, sealing, carrying out standing culture, and regularly stirring to obtain the fruit-vegetable-cereal fermentation beverage after 2-8 months. Compared to the existing Monascus vinegar, the fruit-vegetable-cereal fermentation beverage of the present invention has advantages of short brewing period and high acidity.

The invention relates to an orange lyocell fabric prepared by dyeing a monascus fermentation extract and a preparation method of the orange lyocell fabric. The preparation method comprises the following steps of fermenting monascus, culturing and purifying to obtain monascus orange pigment; and preparing a phenol oxidase solution and a cellulase solution, preparing a dyeing solution from the monascus orange pigment, the phenol oxidase solution, the cellulase solution, a H2O2 solution and the like, putting manganese dioxide, a lyocell fabric and the dyeing solution into a dyeing machine for dyeing, carrying out enzyme deactivation on the dyed lyocell fabric, washing with tap water, and drying to obtain the orange lyocell fabric. According to the invention, cellulose and the monascus orange pigment are respectively activated by selecting cellulase and polyphenol oxidase, so that a graft polymerization reaction of the cellulose and the monascus orange pigment is realized. The dyeing conditions are mild, and the pollution to the environment is small; and the dyed orange lyocell fabric has a relatively high K/S value, and the soaping fastness and the dry rubbing fastness are relatively high in grade.

The invention belongs to the field of biotechnical health-care products, and relates to a monascus rubber Mr-99 strain capable of producing extracellular polysaccharide at high yield through liquid fermentation and application, as well as a polysaccharide preparation method. The monascus rubber Mr-99 strain is preserved in the China Center for Type Culture Collection (CCTCC) on March 2, 2011 with the preservation number of CCTCC No. M2011053. The strain has excellent characteristics of high yield of extracellular polysaccharide, high growing speed, high-temperature resistance and the like. The yield of the extracellular polysaccharide can be 18.6 g/L after liquid fermentation culture medium and fermentation condition optimization.

The invention provides a construction method of a high yield monascorubin strain. The method comprises the steps of connecting a pair of oligonucleotide sequences sequentially comprising Hind III, KpnI, Sac I, Pac I, Pme I, Xho I, Xba I and Bgl II enzyme cutting sites to dual plant plasmid pCambia0380 to construct a double plasmid expression vector pCambia0380G, connecting an hph expression cassette segment with the expression vector to form a double plasmid knockout vector pHph0380, amplifying upstream and downstream homologous arm segments of a gltp1 gene, connecting the homologous arm segments to the double plasmid knockout vector pHph0380 to form a double plasmid knockout vector pHph0380-GLTP, mediating and transforming the vector pHph0380-GLTP from a root knot agrobacterium EHA105 toparent monascus ruber CICC41233 to accomplish construction of the high yield strain. Experimental verification shows that the overall productivity of monascorubin is improved, an alcohol soluble pigment can be accumulated directionally, and at the same time, the accumulation of the monascorubin in a fermentation process is advanced significantly.

The invention relates to a process method for producing uranidin by liquid state fermentation of potatoes with monascus purpureus, which comprises the following steps: cutting fresh potatoes into blocks, adding a proper proportion of water, conducting pulping to form a homogenate, carrying out pretreatment sterilization by using a double-enzyme method, inoculating a monascus purpureus seed solution into the potato homogenate after the potato homogenate is cooled, conducting culturing for a proper time at a proper temperature and a proper shaking frequency, extracting monascus pigment after fermentation is completed, and preparing uranidin to obtain a product.

The invention discloses application of a composite yeast seed in fermentation of a soy sauce. The application is characterized in that each yeast seed in the composite yeast seed is respectively subjected to starter propagation, and inoculated into a clinker of raw materials to prepare the composite yeast after being mixed according to different combinations and ratios, wherein each yeast seed in the composite yeast seed corresponds proper raw materials, so as to prepare a corresponding yeast; the procedures of temperature control at an early stage, yeast turnover, temperature control to yeast discharge are needed in preparation of the composite yeast; the yeast seed comprises at least two of proso millet aspergillus, aspergillus niger and monascus ruber.