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Monascus purpureus strain, and fermentation product thereof and fermentation method thereof

A technique of Monascus violaceum and a fermentation method, applied in the field of fermentation, can solve the problems of adverse effects of production and utilization, difficult production cost, low efficiency, etc., and achieves the effects of low synthesis amount of citrinin, low cost and simple operation

Active Publication Date: 2018-12-18
BEIJING BEINONG HONGZE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the low efficiency of producing lovastatin by Monascus makes it difficult to reduce its production cost. At the same time, Monascus can also produce citrinin, which has a negative impact on its production and utilization.

Method used

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  • Monascus purpureus strain, and fermentation product thereof and fermentation method thereof
  • Monascus purpureus strain, and fermentation product thereof and fermentation method thereof
  • Monascus purpureus strain, and fermentation product thereof and fermentation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The purpose of this example is to separate and purify Monascus purpura strains from red yeast rice for screening high-yielding strains of lovastatin production. The specific experimental method is as follows:

[0036] (1) Grind red yeast rice from Fujian area, inoculate a small amount of red yeast rice on a PDA medium plate with an inoculation loop, and culture at 28°C for 5 days. Separation, protrusion, no cracking, dense aerial hyphae and fine radial folds, and punctate red pigment secretion in the medium on the back) were used as controls for screening.

[0037] (2) Isolation of strains: Take the Monascus purpura that was screened on the PDA plate and grow well, select a single colony, purify and culture it on the slant of the PDA medium test tube, and cultivate it at 28°C for 5 days to obtain the culture on the slant for future use.

[0038] Experimental results: In this example, a total of 30 strains of Monascus purpura were isolated and purified, and the strain c...

Embodiment 2

[0040] The purpose of this example is to detect the lovastatin-producing ability of the 30 strains of Monascus purpura described in Example 1 by high performance liquid chromatography (HPLC). The specific experimental method is as follows:

[0041] (1) Sample preparation

[0042] Take the 30 strains of Monascus purpura obtained from the isolation and purification of Example 1, pick the bacterium blocks with a colony diameter of 0.5 cm on the slant of the PDA medium test tube, and inoculate them in the seed medium respectively. After 3 days of shaking culture at 28 ° C and 160 r / min, Move it into the fermentation medium according to the inoculum size of 7%, and continue to ferment for 7 days under the same conditions.

[0043] After the fermentation, take 2mL of fermentation broth into a 50mL centrifuge tube and add 8mL of anhydrous methanol. Mix well and place on a shaker at 160r / min for 3h extraction, centrifuge at 5000r / min for 10min to take the supernatant, and filter thr...

Embodiment 3

[0057] The purpose of this example is to detect the ability of 30 Monascus purpura strains described in Example 1 to synthesize citrinin by high performance liquid chromatography (HPLC). The specific experimental method is as follows:

[0058] (1) Sample preparation

[0059] The 30 strains of Monascus purpura obtained after separation and purification in Example 1 were respectively inoculated into the seed medium with a 10% inoculation amount, and cultured in a shaker at 28° C. for 3 days. Afterwards, inoculate liquid culture medium according to the inoculum amount of 7%, and shake and cultivate at 160 r / min at 28°C for 10 days.

[0060] After the fermentation, take 2 mL of fermentation broth and add 4 mL of 50% ethanol. Mix well and place in a constant temperature water bath at 60°C for 45 minutes, cool to room temperature, centrifuge to get the supernatant, and filter through an organic filter membrane to obtain the sample to be tested and set aside.

[0061] (2) Utilize ...

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Abstract

The invention relates to a Monascus purpureus strain, and a fermentation product and a fermentation method thereof. The Monascus purpureus has a strain number of ZX26 and an accession number of CGMCCNO. 15992, and has the advantages of high lovastatin yield, low citrinin synthesis amount, compliance with food safety standards and good genetic stability. The fermentation product comprises myceliaproduced by fermentation and a fermented product lovastatin, and has the advantages of high lovastatin content, low citrinin content, compliance with food safety standards and high nutritional and health-care value. The method comprises a step of inoculating the seed solution of the Monascus purpureus strain into a fermentation medium for oscillating fermentation culture. The fermentation method is simple in operation, low in cost, and convenient for large-scale production. Furthermore, after the fermentation method is optimized, the ability of the Monascus purpureus strain in producing lovastatin can be greatly enhanced.

Description

technical field [0001] The invention relates to the field of fermentation, in particular to a strain of Monascus purpura and its fermentation product and fermentation method. Background technique [0002] Lovastatin is a 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor, which is a comprehensive lipid-lowering drug, which can competitively inhibit the endogenous cholesterol synthesis rate-limiting enzyme HMG-CoA Reductase, blocking intracellular valonate metabolic pathway, reducing intracellular cholesterol synthesis, thereby stimulating the increase in the number and activity of low-density lipoprotein (LDL) receptors on the cell membrane surface in a feedback manner, increasing serum cholesterol clearance and lowering the level Clinical It is mainly used to lower cholesterol, especially low-density lipoprotein-cholesterol (LDL-C). Due to its advantages of safe use, significant curative effect, and less adverse reactions in the prevention and treatment of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P17/06C12R1/645
CPCC12N1/14C12P17/06C12N1/145C12R2001/645
Inventor 张红星谢远红刘慧金君华
Owner BEIJING BEINONG HONGZE BIOTECH CO LTD
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