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High yield gamma-reanal monascus ruber Mr-5 bacterial strain, screening method and use thereof

A technology of Monascus rubrum and aminobutyric acid, applied in the field of biotechnology food, can solve the problems of lack of safety in chemical synthesis, low GABA content, inappropriateness and the like

Inactive Publication Date: 2010-12-08
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its GABA content is very low, and it is very difficult to extract GABA from these raw materials as food additives or health products
The chemical synthesis of GABA is not suitable as a food additive or medical GABA due to lack of safety

Method used

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  • High yield gamma-reanal monascus ruber Mr-5 bacterial strain, screening method and use thereof
  • High yield gamma-reanal monascus ruber Mr-5 bacterial strain, screening method and use thereof
  • High yield gamma-reanal monascus ruber Mr-5 bacterial strain, screening method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Monascus ruber Mr-5 bacterial strain screened from red yeast rice

[0027] The gradient dilution separation method was used. Red yeast rice (10mg) was ground into powder, and carried out gradient dilution (10 -1 -10 -4 ), choose the appropriate concentration (10 -2 ) 200 μL coated plate, cultivated at 25°C, picked early colonies that were white (about 3 days), and later single colonies that were red or purple (about 5 days), were continuously streaked and purified to obtain pure red Monascus Mr. -5 strains, numbered, stored in a refrigerator at 4°C, for later use. Use PDA solid slant medium to activate and subculture the strains, and transfer the strains every 4 weeks; the strains need to be activated before use.

[0028] The colony characteristic of the above-mentioned Monascus ruberus Mr-5 strain is 25 DEG C cultured on the malt extract agar (MEA) medium for 7 days, the diameter of the colony is 32 mm, the texture is loose, the edge is irregular, and the...

Embodiment 2

[0031] The method that embodiment 2 Monascus ruber Mr-5 strain is used for the synthesis of gamma-aminobutyric acid

[0032] (1) culture medium

[0033] PDA medium: 200g potato, 20g glucose, 20g agar powder, 1000mL water, pH 5.5.

[0034] PD culture medium: 200g of potatoes, 20g of glucose, 1000mL of water, pH 5.5.

[0035] Bean sprout juice medium: 200g bean sprouts, 20g glucose, 20g agar powder, 1000mL water, pH 5.5.

[0036] Wheat flour fermentation medium: 50g wheat flour, 25g sodium nitrate, 5g sodium glutamate, 1g zinc sulfate, 1g calcium chloride, initial pH 5.5.

[0037] Sweet potato starch fermentation medium: sweet potato starch 50g, sodium nitrate 25g, sodium glutamate 5g, zinc sulfate 1g, calcium chloride 1g, initial pH 5.5.

[0038](2) Synthetic method

[0039] Take the Monascus ruberus Mr-5 strain to preserve the strain, under sterile conditions (sterile room or ultra-clean workbench), pick a little strain with an inoculation needle, transfer it to a petri di...

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Abstract

The invention belongs to the biotechnology food field, and in particular relates to a high-yield gamma-aminobutyric acid Monascus ruber Mr-5 bacterial strain, as well as a screening method and a use of the same. The preservation number of the high-yield GABA Monascus ruber Mr-5 bacterial strain is CCTCC No: M208043, which is preserved in China Center for Type Culture Collection. In addition, the invention also discloses a screening method and a use of the Monascus ruber Mr-5 bacterial strain, as well as a method of using the Monascus ruber Mr-5 bacterial strain for the synthesis of gamma-aminobutyric acid. A fermentation liquid obtained through the method of synthesizing the gamma-aminobutyric acid contains 6-9 g of the gamma-aminobutyric acid per liter by biological methods. Moreover, the produced gamma-aminobutyric acid has no potential safety trouble and can reach the GRAS.

Description

technical field [0001] The invention belongs to the field of biotechnology food, and in particular relates to a high-yield γ-aminobutyric acid Monascus ruber (Monascus ruber Mr-5) strain, a screening method and application thereof. Background technique [0002] γ-aminobutyric acid (GABA for short) is a naturally occurring active amino acid and an important inhibitory neurotransmitter in the mammalian central nervous system. Memory, control asthma, regulate hormone secretion, promote reproduction, strengthen liver and kidney, relieve menopausal syndrome and other physiological functions. Foods rich in GABA have good immune health effects and can be used as a functional food. [0003] GABA is widely distributed in animals, plants and microorganisms. Enrichment of GABA with edible plants (such as tea, mulberry leaves), grains (such as rice, rice germ), and agricultural by-products (such as rice bran) can achieve GABA fortification of foods processed with these raw materials. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12Q1/04C12P13/00C12R1/66
Inventor 蒋冬花后家衡李杰
Owner ZHEJIANG NORMAL UNIVERSITY
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