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Monascus rubber Mr-99 strain and its application

A technology of Monascus red, mr-99, which is applied in the direction of fungi, microorganisms, biochemical equipment and methods, etc., and can solve the problems of lack of high-yielding red yeast polysaccharide strains and imperfect fermentation production processes, etc.

Active Publication Date: 2011-12-14
杭州双马生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the increasing demand for red yeast polysaccharides in the market, the lack of high-yield red yeast polysaccharide strains and the imperfect fermentation process

Method used

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  • Monascus rubber Mr-99 strain and its application
  • Monascus rubber Mr-99 strain and its application
  • Monascus rubber Mr-99 strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Isolation, purification and screening of Monascus rubra Mr-99 strain

[0036] 1.1 Medium

[0037] (1) PDA medium: 200 g potato, 20 g glucose, 18 g agar powder, 1000 mL water, natural pH;

[0038] (2) Cha's synthetic medium (seed medium): glucose 30 g, NaNO 3 3 g, yeast extract 1 g, K 2 HP0 4 1 g, KCl 0.5 g, MgS0 4 .7H 2 O 0.5 g, FeS0 4 ·7H 2 0.01 g, 1000 mL water, pH 5.0;

[0039] (3) Fermentation broth: 50 g glucose, 20 g peptone, KH 2 PO 4 8 g, MgS0 4 .7H 2 O 0.5 g, KCl 0.5 g, deionized water 1000 mL, pH 6.0;

[0040] (4) CYA medium (refer to Li Zhongqing et al., 2004, for identification): sucrose 30.0 g, NaNO 3 3 g, yeast extract 5.0 g, K 2 HPO 4 1 g, KCl 0.5 g, MgSO 4 . 7H 2 O 0.05 g, FeSO 4 . 7H 2 O 0.01 g, agar 18 g, water 1000 mL, pH 6.0.

[0041] Separation, purification and screening

[0042] The red yeast strain Mr-99 is isolated from naturally fermented red yeast rice. Grind 0.5 g of red yeast rice into a powder with a mortar, pour a small amount even...

Embodiment 2

[0043] Example 2 The process flow of fermentation production and preparation of extracellular polysaccharide

[0044] Take the Monascus rubrum Mr-99 strain to preserve the strains, under aseptic conditions (sterile room or ultra-clean workbench), pick a few strains with an inoculation needle, transfer to a culture plate containing PDA, and activate culture at 30 ℃ 108 h. Connect the activated strains to the PDA culture plate in the same way, and cultivate them at 30 ℃ for 120 h, which is the first-class seed. Fill a 250 mL Erlenmeyer flask with 50 mL of Cha's synthetic culture solution, insert 5 pieces of 1cm diameter Mr-99 strain first-level seed bacteria cake, 32 ℃ shaker (200 r / min) culture for 96 h, that is the second-level fermentation seed . According to the scale of fermentation, for example, a 3.7 L fermenter is filled with 2.5L of fermentation broth, the inoculum is 8% (V / V), the speed is 200 r / min, the temperature is 32 ℃, the aeration rate is 100 L / h, and the tank ...

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Abstract

The invention belongs to the field of biotechnical health-care products, and relates to a monascus rubber Mr-99 strain capable of producing extracellular polysaccharide at high yield through liquid fermentation and application, as well as a polysaccharide preparation method. The monascus rubber Mr-99 strain is preserved in the China Center for Type Culture Collection (CCTCC) on March 2, 2011 with the preservation number of CCTCC No. M2011053. The strain has excellent characteristics of high yield of extracellular polysaccharide, high growing speed, high-temperature resistance and the like. The yield of the extracellular polysaccharide can be 18.6 g / L after liquid fermentation culture medium and fermentation condition optimization.

Description

technical field [0001] The invention belongs to the field of biotechnology health care products, and relates to a Monascus ruberus Mr-99 strain with high yield of exopolysaccharide by liquid fermentation, its application and a polysaccharide preparation method. Background technique [0002] Fungal polysaccharides are polymers isolated from fungal fruiting bodies, mycelia, and fermentation broths, which are composed of more than 10 monosaccharides linked by glycosidic bonds. They have the ability to control cell division and differentiation, regulate cell growth and aging, and enhance A class of active polysaccharides with functions such as body immunity. Since Qianyuan Haneda, Japan isolated an anti-tumor polysaccharide from shiitake mushrooms in 1970, the scientific community has set off an upsurge in the study of edible fungal polysaccharides. At present, the fungal polysaccharides that have been clinically used in China mainly include: lentinan, versicolor polysaccharide,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P19/04C12R1/645
Inventor 蒋冬花汪鹏荣刘伟芬曹丽凌
Owner 杭州双马生物科技股份有限公司
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