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Method for preparing open-loop lovastatin

A lovastatin, ring-opening technology, which is applied in the field of preparation of ring-opening lovastatin from Monascus to achieve the effects of less pollution, mild working conditions and simple equipment

Inactive Publication Date: 2010-03-03
BEIJING WBL PEKING UNIV BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of converting closed-loop lovastatin into open-loop lovastatin generally adopts chemical method, and closed-loop lovastatin will be converted into open-loop lovastatin within a certain period of time under alkaline conditions; at present, there is no microbial biotransformation of closed-loop lovastatin Reports for open-loop lovastatin

Method used

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  • Method for preparing open-loop lovastatin
  • Method for preparing open-loop lovastatin
  • Method for preparing open-loop lovastatin

Examples

Experimental program
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Effect test

Embodiment 1

[0045] A, select the red yeast rice strain CGMCC NO.0272 to cultivate, and insert the fermentation medium to ferment;

[0046] Slant strain culture: add wort, sugar, and agar with a concentration of 4 degrees to water to make a medium with a Ph of 7, so that each ml of medium contains 0.15g of white sugar and 0.06g of agar; sterilize at 110°C , cooled after sterilization, made into a slant, inoculated, and cultivated at 30°C for 5 days as the slant strain;

[0047] Seed culture and fermentation: Add glucose, beef extract, peptone, ammonium nitrate, and magnesium sulfate to water to make a medium with a pH of 7, so that each ml of medium contains 0.05g of glucose, 0.05g of beef extract, 0.03g of peptone, and ammonium nitrate 0.03g, magnesium sulfate 0.04g; put 60ml of medium into a 250ml tripod bottle, sterilize at 110°C for 30 minutes, cool down after sterilization, insert the slant bacteria so that the volume of the slant bacteria occupies the medium 7%, at 30°C, shake flask...

Embodiment 2

[0054] A, select the red yeast rice strain CGMCC NO.0272 to cultivate, and insert the fermentation medium to ferment;

[0055] Slant strain culture: add wort, sugar, and agar with a concentration of 4 degrees to water to make a medium with a Ph of 7, so that each ml of medium contains 0.15g of white sugar and 0.06g of agar; sterilize at 110°C , cooled after sterilization, made into a slant, inoculated, and cultivated at 30°C for 5 days as the slant strain;

[0056] Seed cultivation and fermentation: Add glucose, beef extract, peptone, ammonium nitrate, and magnesium sulfate to water to make a medium with a pH of 7, so that each ml of medium contains 0.05g of glucose, 0.05g of beef extract, 0.03g of peptone, and nitric acid Ammonium 0.03g, magnesium sulfate 0.04g; put 60ml of culture medium in a 250ml tripod bottle, sterilize at 110°C for 30 minutes, cool down after sterilization, insert the slant bacteria so that the volume of the slant bacteria occupies 7% of the culture med...

Embodiment 3

[0063] A, select the red yeast rice strain CGMCC NO.0272 to cultivate, and insert the fermentation medium to ferment;

[0064] Slant strain culture: add wort, sugar, and agar with a concentration of 4 degrees to water to make a medium with a Ph of 8, so that each ml of medium contains 0.1g of white sugar and 0.08g of agar; sterilize at 100°C , cooled after sterilizing, made into a slant, inoculated, and cultivated at 20°C for 6 days as the slant strain;

[0065] Seed cultivation and fermentation: Add glucose, beef extract, peptone, ammonium nitrate, and magnesium sulfate to water to make a medium with a pH of 6, so that each ml of medium contains 0.08g of glucose, 0.03g of beef extract, 0.02g of peptone, and nitric acid Ammonium 0.04g, magnesium sulfate 0.06g; put 40ml of medium into a 250ml tripod bottle, sterilize at 110°C for 20 minutes, cool down after sterilization, insert the slant bacteria, so that the volume of the slant bacteria occupies 9% of the culture medium was ...

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Abstract

The invention discloses a method for preparing open-loop lovastatin by using monascus purpureus, which is a biological conversion method for utilizing monascus to produce enzyme and hydrolyze closed-loop lovastatin into the open-loop lovastatin. The method comprises the following steps: selecting and culturing a monascus strain CGMCC NO.0272; putting the cultured monascus strain CGMCC NO.0272 intoa fermentation medium for fermentation; preparing crude enzyme solution by a salting out method; performing substrate preparation; performing enzymatic conversion reaction; and performing other steps. The method for preparing the open-loop lovastatin by using the monascus purpureus adopts microbial conversion means, and compared with chemical means, the microbial conversion means has the advantages of moderate condition, simple equipment, less public hazard, quick reaction rate and relatively environmental protection.

Description

technical field [0001] The invention relates to a method for preparing lovastatin, in particular to a method for preparing ring-opening lovastatin by using monascus. Background technique [0002] Lovastatin (LOVASTATIN), chemical name: (S)-2-methylbutanoic acid-(1S,3S,7S,8S,8aR)-1,2,3,7,8,8a-hexahydro-3 , 7-dimethyl-8-{2-[(2R,4R)-4-hydroxyl-6 oxo-2-tetrahydropyranyl]-ethyl}-1-naphthyl ester; molecular formula is C 24 h 36 o 5 , molecular weight 404.55. [0003] Lovastatin is a fermentation product of Monascus and Aspergillus terreus, a 3-hydroxy-3-methylpentanediate coenzyme A (HMGCoA) reductase inhibitor, which has a very obvious effect of lowering blood lipids. Widely used in clinical and health products. Lovastatin has two structural forms of open ring and closed ring. Because the closed ring structure is relatively stable, it is used as the structural form of chemical medicine products for clinical use. In 1987, Merck's lipid-lowering drug lovastatin (lovastatin, M...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P17/06C12R1/66
Inventor 段震文郭树仁何大林巩金妹
Owner BEIJING WBL PEKING UNIV BIOTECH
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