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Method for preparing monascus red pigment by utilizing immobilized enzymes

A technology of monascus red pigment and immobilized enzyme, which is applied in the field of preparing monascus red pigment by using immobilized enzymes, can solve the problems of high cost, complicated purification and enrichment process, difficult separation and recovery of enzyme liquid, etc., to improve efficiency and save The effect of increasing resources and stability

Active Publication Date: 2013-04-03
山东中惠生物科技股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Effectively disperse, crush, emulsify, and mix the fermentation liquid to improve the efficiency of subsequent enzymatic hydrolysis, and at the same time fully release the red pigment in the bacteria to improve the extraction rate of the pigment; and solve the problem that the enzyme liquid is not easy to separate and recover in the traditional process , the purification and enrichment process is complicated, the cost is high, the product color price is low, and the utilization rate of resources can be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Preparation of immobilized enzyme

[0048] Mix 35 g of sodium alginate and 25 g of gelatin, add it to 1L of water, stir to dissolve at 45°C, add 35g of complex enzyme, stir and mix well, shake on a constant temperature shaker at 45°C, 100r / min for 0.5h , and then inject the mixed solution dropwise into 1.5mol / L calcium chloride solution, let it stand for 50min at 4°C, make it condense and solidify, collect the gel beads, and add 0.2mol / L sodium chloride The solution was washed, and the free water was removed by suction filtration, and the gel beads were collected to obtain the immobilized enzyme.

[0049] (2) Preparation of Monascus Red Pigment

[0050] Put the liquid fermentation liquid of Monascus into a colloid mill for homogenization treatment, the crushing fineness is 5 μm, and the processing time is 30 minutes; after the homogenization, adjust the solution to pH 4.5, according to the mass ratio of wet weight to homogenization liquid Add the immobilized co...

Embodiment 2

[0052] (1) Preparation of immobilized enzyme

[0053] Mix 4g of sodium alginate solution and 2g of denatured starch, add it to 100ml of water, stir to dissolve at 45°C, add 2g of compound enzyme, stir and mix well, shake on a constant temperature shaker at 37°C, 60r / min for 1.5 h, then inject the mixed solution dropwise into 1.5mol / L calcium chloride solution, let it stand at 4°C for 110min, make it condense and solidify, collect the gel beads, and use 0.2mol / L calcium chloride solution Wash with sodium solution, remove free water by suction filtration, collect gel beads to obtain immobilized enzyme.

[0054] (2) Preparation of Monascus Red Pigment

[0055] Put the liquid fermentation liquid of Monascus into a colloid mill for homogenization treatment, the crushing fineness is 1 μm, and the processing time is 20 minutes; after the homogenization, adjust the solution to pH5. Add the immobilized compound enzyme at a ratio of 1:30, and then filter the enzyme after 6 hours of...

Embodiment 3

[0057] (1) Preparation of immobilized enzyme

[0058] Add 100g of sodium alginate to 8L of water, stir and dissolve at 40°C, add 50g of compound enzyme, stir and mix well, vibrate on a 37°C constant temperature shaker at 90r / min for 0.6h, and inject the mixture dropwise into 2.0 mol / L calcium chloride solution, let it stand at 3°C ​​for 120min to coagulate and solidify, collect the gel beads, wash with 0.15mol / L sodium chloride solution, remove free water by suction filtration, collect the gel beads Glue beads get immobilized enzymes.

[0059] (2) Preparation of Monascus Red Pigment

[0060] Put the liquid fermentation liquid of Monascus into a colloid mill for homogenization treatment, the crushing fineness is 4 μm, and the processing time is 60 minutes; after the homogenization, adjust the solution to pH4.5, according to the mass ratio of wet weight to homogenization Add the immobilized complex enzyme in the amount of 1:40, and filter it after 5 hours of enzymolysis on ...

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Abstract

The invention relates to a method for preparing a monascus pigment, in particular to a method for preparing a monascus red pigment by utilizing immobilized enzymes. The method comprises the steps as follows: taking a monascus liquid fermentation solution as a raw material, homogenizing, enzymatically hydrolyzing the immobilized enzymes, alkalifying and filtering to obtain a monascus red pigment solution; and carrying out acid deposition on the monascus red pigment solution, washing and neutralizing the deposits, dehydrating and drying to obtain the monascus red pigment. The method has the advantages as follows: on one hand, the fermentation solution is effectively dispersed, broken, emulsified and mixed by utilizing a colloid mill, so that the subsequent enzymatic hydrolysis efficiency is improved and the monascus red pigment in monascus is fully released; and on the other hand, an enzyme preparation can be recycled, the reaction conditions are moderate, the monascus red pigment is concentrated through the acid deposition method, the process is simple, the energy consumption is low, and the product is easy to separate. The production cost can be remarkably reduced through the monascus red pigment prepared with the method. The monascus red pigment has the maximum absorption wavelength of 499 nm, is good in water solubility and light, heat, acid, alkali and metal stabilities, and is favorable for the monascus pigment industry development.

Description

[0001] technical field [0002] The invention relates to a preparation method of monascus red pigment, in particular to a method for preparing monascus red pigment by using immobilized enzyme. Background technique [0003] With the development of the food industry and the improvement of people's living standards, food pigments have been paid more and more attention as an important part of food additives. Especially in recent years, with the national attention to food safety issues, the use of It is the trend of the times to replace natural pigments with synthetic pigments. As a deep-processing product of agricultural products, monascus red pigment has high safety and good stability, and has good development and application prospects. [0004] Chinese invention patent ZL200510044948.8 discloses a method for extracting water-soluble red yeast rice pigments by enzymatic hydrolysis. The method for extracting water-soluble red yeast rice pigments by enzymatic hydrolysis in the p...

Claims

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Application Information

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IPC IPC(8): C09B61/00
Inventor 赵吉兴周庆新
Owner 山东中惠生物科技股份有限公司
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