Preparation method of high-purity monascus pigment component
A red yeast pigment, high-purity technology, applied in the field of biochemical products, can solve the problems of small processing capacity, difficult separation and purification of red yeast pigment, and achieve the effect of simple operation steps, saving the consumption of separation materials and high yield
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[0021] Example 1
[0022] The value of red yeast rice is 5100U / g, containing red pigment (erythematine and monascus red amine) 10.1g / Kg, orange pigment (erythematine and monascus red pigment) 48.3g / Kg, yellow pigment (monascus and monascus Flavin) 41.9g / Kg. After crushing to a particle size of 0.1mm, using 70% (weight ratio, the same below) ethanol solution extraction (60℃ water bath, solid-liquid ratio 1:10), vacuum concentration to 1 / 2 the original volume to precipitate the crude monascus pigment extract , The yield is 10.1%.
[0023] A high-speed countercurrent chromatography system with a column volume of 300 mL was used for separation. Select n-hexane-methanol-water as the solvent system, according to the ratio of 10:7.5:2.5 (v:v:v:v), dispose the above solvent components in the separatory funnel, shake well and stand for stratification. Take the upper layer as the stationary phase and the lower layer as the mobile phase.
[0024] Weigh 200 mg of crude monascus pigment extr...
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[0027] Example 2
[0028] Red yeast rice has a color price of 3000U / g, crushed to a particle size of 1mm, extracted with 70% (weight ratio, the same below) methanol solution (60℃ water bath, solid-liquid ratio 1:15), vacuum concentrated to 1 / 3 of the original volume. The crude monascus pigment was separated out, and the yield was 6.7%.
[0029] A high-speed countercurrent chromatography system with a column volume of 1000 mL was used for separation. Select petroleum ether-ethyl acetate-ethanol-water as the solvent system, according to the ratio of 2.5:7.5:5:5 (v:v:v:v), dispose the above solvent components in the separatory funnel, shake well and then stand still 置layered. Take the upper layer as the stationary phase and the lower layer as the mobile phase.
[0030] Weigh 800mg of the crude monascus pigment extract and dissolve it with 50mL stationary phase for use. Before injection, fill the entire column volume with the stationary phase, adjust the host speed to 900rpm / min, pum...
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[0032] Example 3
[0033] Red yeast rice has a rice color price of 4000U / g, crushed to a particle size of 1mm, and extracted with a 90% acetone aqueous solution (60°C water bath, solid-liquid ratio 1:5), and the extracted extract is vacuum concentrated to 1 / 3 of the original volume to precipitate red yeast The crude extract of the pigment component has a yield of 8.5%.
[0034] A high-speed countercurrent chromatography system with a column volume of 1000 mL was used for separation. Choose cyclohexane-chloroform-isopropanol-water as the solvent system, according to the ratio of 5:6:8:2.5 (v:v:v:v) to configure the above solvent components in the separatory funnel, shake well Let stand for layering. Take the upper layer as the stationary phase and the lower layer as the mobile phase.
[0035] Weigh 800 mg of the crude monascus pigment extract and dissolve it with 50 mL of stationary phase for later use. Before injection, fill the entire column volume with the stationary phase, a...
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