Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of high-purity monascus pigment component

A red yeast pigment, high-purity technology, applied in the field of biochemical products, can solve the problems of small processing capacity, difficult separation and purification of red yeast pigment, and achieve the effect of simple operation steps, saving the consumption of separation materials and high yield

Inactive Publication Date: 2010-10-20
FUZHOU UNIVERSITY
View PDF0 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dai Chunhua[4] used silica gel column chromatography to separate monascus yellow pigment, which has a small processing capacity and is difficult to separate and purify single component monascus pigment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of high-purity monascus pigment component
  • Preparation method of high-purity monascus pigment component
  • Preparation method of high-purity monascus pigment component

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Monascus rice color price 5100U / g, containing red pigment (erythromycin and monascus amine) 10.1g / Kg, orange pigment (erythematin and monascus red pigment) 48.3g / Kg, yellow pigment (monascus and monascus Flavin) 41.9g / Kg. Crushed to a particle size of 0.1mm, extracted with 70% (weight ratio, the same below) ethanol solution (water bath at 60°C, solid-to-liquid ratio 1:10), concentrated in vacuum to 1 / 2 of the original volume, and the crude extract of monascus pigment was precipitated , The yield is 10.1%.

[0023] A high-speed countercurrent chromatography system with a column volume of 300 mL was used for separation. Select n-hexane-methanol-water as the solvent system, and arrange the above-mentioned solvent components in a separatory funnel according to the ratio of 10:7.5:2.5 (v:v:v:v), shake well and let stand to separate layers. Take the upper layer as the stationary phase and the lower layer as the mobile phase.

[0024] Weigh 200 mg of the crude extract of Mo...

Embodiment 2

[0028] The color price of red yeast rice is 3000U / g, crushed to a particle size of 1mm, extracted with 70% (weight ratio, the same below) methanol solution (60°C water bath, solid-to-liquid ratio 1:15), and vacuum concentrated to 1 / 3 of the original volume. The crude extract of monascus pigment was precipitated, with a yield of 6.7%.

[0029] A high-speed countercurrent chromatography system with a column volume of 1000 mL was used for separation. Select petroleum ether-ethyl acetate-ethanol-water as the solvent system, and arrange the above solvent components in a separatory funnel according to the ratio of 2.5:7.5:5:5 (v:v:v:v), shake well and let it stand. Set layers. Take the upper layer as the stationary phase and the lower layer as the mobile phase.

[0030] Weigh 800 mg of the crude extract of Monascus pigment, and dissolve it in 50 mL of stationary phase for later use. Before sample injection, fill the entire column volume with the stationary phase, adjust the speed...

Embodiment 3

[0033] The color price of red yeast rice is 4000U / g, crushed to a particle size of 1mm, and extracted with 90% acetone aqueous solution (60°C water bath, solid-to-liquid ratio 1:5), and the extracted extract is concentrated in vacuum to 1 / 3 of the original volume, and the red yeast rice is precipitated The crude extract of the pigment component has a yield of 8.5%.

[0034] A high-speed countercurrent chromatography system with a column volume of 1000 mL was used for separation. Select cyclohexane-chloroform-isopropanol-water as the solvent system, and arrange the above-mentioned solvent components in a separatory funnel according to the ratio of 5:6:8:2.5 (v:v:v:v), shake well Let stand to layer. Take the upper layer as the stationary phase and the lower layer as the mobile phase.

[0035] Weigh 800 mg of the crude extract of Monascus pigment, and dissolve it in 50 mL of stationary phase for later use. Before sample injection, fill the entire column volume with the station...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a novel process for preparing high-purity monascus pigment. The process comprises the following steps of: crushing red yeast rice as a raw material; and separating and purifying to obtain 6 kinds of monascus pigment components by adopting a composite technology of extraction liquid extraction and high-speed countercurrent chromatography separation and crystallization, wherein the six monascus pigment components comprise rubropunctamine, monascorubramine, monascin, ankaflavin, rubropunctatin and monascorubin, and the purities of all the pigments are higher than 95 percent and can be used for preparing foods, cosmetic colorants and health products. The invention adopts easily-recovered organic solvent for separation to save the consumption of separation materials andovercome the problems of small treatment amount and inefficient single pigment component separation and purification of a TLC (Thin Layer Chromatography), an HPLC (High Performance Liquid Chromatography), a resin method and a silica gel column chromatography. The invention adopts the composite technology of the extraction liquid extraction and the high-speed countercurrent chromatography separation and the crystallization to prepare six kinds of high-purity monascus pigment crystals and has the advantages of simple steps and high yield.

Description

technical field [0001] The invention belongs to the technical field of biochemical products, and in particular relates to a preparation method of high-purity monascus pigment. Background technique [0002] High-speed counter-current chromatography (High-Speed ​​Counter-Current Chromatography, HSCCC) is a chromatography that uses the difference in the distribution coefficient of the solute in two immiscible solvent systems to separate. High-speed countercurrent chromatography technology does not require solid-phase support, avoiding sample loss and denaturation caused by irreversible adsorption. The organic solvent used for separation is easy to recycle and use, and saves the consumption of separation materials. This technology has been widely used in the separation and preparation of flavonoids, alkaloids, plant polyphenols, esters, terpenes, lignans, coumarins, saponins and other natural phytochemical components and antibiotics. The preparation of six high-purity monascus ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C09B61/00C09B67/54
Inventor 郑允权郭养浩石贤爱
Owner FUZHOU UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com