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Nano chitosan derivative and preparation method and application thereof

A technology of chitosan derivatives and nano-chitosan, which is applied in the field of biomedical nanomaterials to achieve the effects of stable properties, strong adsorption capacity, and good biocompatibility

Inactive Publication Date: 2009-11-11
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the enrichment and purification of glycosylated polypeptides / proteins using chitosan derivatives with active boronic acid functional groups as immobilized substrates

Method used

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  • Nano chitosan derivative and preparation method and application thereof
  • Nano chitosan derivative and preparation method and application thereof
  • Nano chitosan derivative and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: the preparation of chitosan-GMA-phenylboronic acid

[0034] 1) Preparation of chitosan-GMA epoxy intermediate (chitosan-GMA)

[0035]In a 100mL three-necked flask equipped with a stirrer, a thermometer and a condenser tube, 0.5 g of chitosan (Qingdao Haihui Biological Co., Ltd., deacetylation degree 91%) was dissolved in 30 mL of an aqueous solution containing 2 wt% of acetic acid, and 0.5 mL of glycidyl methacrylate, stirred, then added 0.035 g of ammonium persulfate and 0.035 g of sodium thiosulfate, heated to 50 ° C, reacted for 2 hours, stopped the reaction, cooled to room temperature, centrifuged to remove the supernatant, It was washed with water to obtain a solid.

[0036] 2) Preparation of chitosan-GMA-phenylboronic acid (chitosan-GMA-APB)

[0037] In the 100mL there-necked flask equipped with stirrer, thermometer and condensing tube, load the solid matter prepared in step 1, add 0.5 gram of 3-aminophenylboronic acid, 0.25 gram of sodium chloride...

Embodiment 2

[0039] Embodiment 2: the same as the method of embodiment 1, wherein raw material glycidyl acrylate replaces glycidyl methacrylate.

Embodiment 3-4

[0040] Embodiment 3-4: The method is the same as that of Embodiment 1, except that the initiators therein are azobisisobutyronitrile and n-butyllithium respectively.

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PUM

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Abstract

The invention discloses a nano chitosan derivative and a preparation method thereof, belonging to the technical field of biomedical nano materials. The preparation method of the derivative comprises the following steps of: dissolving the chitosan in dilute acid and then mixing the chitosan with methacrylic acid epoxy propyl ester or acrylic acid epoxy propyl ester so as to conduct self-polymerization grafting reaction, thereby obtaining an intermediate with an active epoxy group, then leading the intermediate and an amino group to replace phenylboronic acid to carry out ring-opening addition reaction for finally obtaining the nano chitosan derivative with a boric acid active functional group, with the particle size of 1nm to 300nm. The invention also discloses the application of the chitosan derivative in the aspect of enriching and purifying glycosylated polypeptide / protein. The chitosan derivative has very high specificity, can be used for the enrichment and purification of low-abundance glycosylated polypeptide / protein in biological samples, and can also be used in the biological and medical fields, including clinical diagnosis.

Description

technical field [0001] The invention relates to the technical field of biomedical nanometer materials. Specifically, it relates to a nano-chitosan derivative, a preparation method, and a method for using the nano-chitosan derivative to enrich and purify glycosylated polypeptide / protein with high selectivity and specificity. Background technique [0002] Post-translational protein modification is a hot research topic in proteomics. Protein glycosylation is the most common and important post-translational modification of proteins. Protein glycosylation regulates almost the entire process of life activities, including cell proliferation, development and differentiation, metabolism, immune response, tumor Some glycosylated proteins are used as relevant targets or biomarkers for the treatment of diseases for the early detection and identification of cancer, such as carcinoembryonic antigen for the detection of rectal cancer, breast cancer, prostate cancer and lung cancer; CA-125...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08C07K1/22
Inventor 邹霞娟娄雅欣钟丽君刘丹杨彬彭嘉柔
Owner PEKING UNIV
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