A sterilization composition containing a compound named as Chinese characters 'Pujuxitang' and ethylicin is provided. The composition includes effective components which are the 'Pujuxitang' and the ethylicin and which are compounded, with the balance being auxiliary components. The mass ratio of the effective components which are the 'Pujuxitang' and the ethylicin in the composition is 1-50:50-1. A preparation includes 1-80% by mass of the effective components which are the 'Pujuxitang' and the ethylicin, with the balance being auxiliary components permissable and acceptable in pesticides. The dosage form of the pesticide composition is an emulsifiable solution, a suspension, wettable powder, water-dispersible granules, an emulsion in water, a microemulsion, granules and a microcapsule. The composition is used for control of fruit tree rot disease, alternaria leaf spot, rise blast, soybean root rot, wilt of cucurbits, cotton verticillium wilt, rust disease of jujube trees, grape white rot, black spot of ginseng and panax pseudoginseng, tea gall of tea leaves, forest rot disease, canker, gummosis, leaf cast, forest seedling-stage blight, pathogenic bacteria for vegetable soft rot, pathogenic bacteria of cucumber angular leaf spot, pathogenic bacteria of rice bacterial leaf blight, pathogenic bacteria of apple ring spot, pathogenic bacteria of wheat scab, and the like.