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Testing cotton swab as well as preparation method and testing method thereof

A technology of cotton swabs and swabs, which is applied in the field of medical devices, can solve the problems of non-rapid detection of drug sensitivity, troublesome operation, and high cost, and achieve the effects of shortening the time for proliferation and culture, simple structure, and low production and use costs

Inactive Publication Date: 2016-01-20
GUANGDONG HAID GROUP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In a word, the existing routine bacterial drug susceptibility testing methods have the following disadvantages: (1) the whole testing process takes a long time, (2) the operation is troublesome, (3) the cost is high, and it is almost impossible to be used for rapid drug susceptibility in aquaculture. detection

Method used

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  • Testing cotton swab as well as preparation method and testing method thereof
  • Testing cotton swab as well as preparation method and testing method thereof
  • Testing cotton swab as well as preparation method and testing method thereof

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preparation example Construction

[0038] Another object of the present invention is to provide a method for preparing a test cotton swab, by which a test cotton swab is obtained for testing bacterial drug susceptibility; the preparation method comprises the following steps:

[0039] 1) Preparation of antibiotic-drug susceptibility test cotton swab: the antibiotic is formulated into a solution, filtered with a 0.1-0.5 μm filter to obtain an antibiotic solution, and then the cotton swab head of the autoclaved cotton swab is aseptically prepared Insert into the above antibiotic solution;

[0040] 2) Soaking medium: add double distilled water to the solid medium and mix, then autoclave, when the temperature of the medium drops to 50-60°C, transfer it to an EP centrifuge tube, and put the antibiotic-medicine prepared in step 1) The cotton swab head of the sensitive test cotton swab is inserted into the culture medium for 1-3 seconds, taken out and placed in a sterile petri dish to cool, avoiding the contact between...

Embodiment 1

[0050] A kind of test cotton swab, it comprises cotton swab handle and cotton swab head, after described cotton swab head is immersed in antibiotic, inserts in solid culture medium and forms solid culture medium layer on the surface of cotton swab head; This test cotton swab passes Prepared by the following method:

[0051] 1) Preparation of antibiotic-drug susceptibility test cotton swab: prepare enrofloxacin into a solution with a concentration of 1-10 μg / ml, filter it with a 0.1 μm filter to obtain an antibiotic solution, and then sterilize it by autoclaving under sterile conditions Insert the cotton swab head of the cotton swab into the above solution;

[0052] 2) Soaking medium: add double distilled water to the solid medium and mix, then autoclave, when the temperature of the medium drops to 50-60°C, transfer it to an EP centrifuge tube, and put the antibiotic-medicine prepared in step 1) Insert the cotton swab head of the sensitive test cotton swab into the medium for ...

Embodiment 2

[0055] A kind of test cotton swab, it comprises cotton swab handle and cotton swab head, after described cotton swab head is immersed in antibiotic, inserts in solid culture medium and forms solid culture medium layer on the surface of cotton swab head; This test cotton swab passes Prepared by the following method:

[0056] 1) Preparation of antibiotic-drug susceptibility test cotton swab: Florfenicol is prepared into a solution with a concentration of 10-50 μg / ml, filtered with a 0.22 μm filter to obtain an antibiotic solution, and then sterilized by autoclaving under aseptic conditions. Insert the cotton swab head of the cotton swab into the above solution;

[0057] 2) Soaking medium: add double distilled water to the solid medium and mix, then autoclave, when the temperature of the medium drops to 50-60°C, transfer it to an EP centrifuge tube, and put the antibiotic-medicine prepared in step 1) Insert the cotton swab head of the sensitive test cotton swab into the medium f...

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Abstract

The invention discloses a testing cotton swab as well as a preparation method and a testing method thereof. The testing cotton swab comprises a cotton swab handle and a cotton swab head, wherein the cotton swab head is inserted into a solid culture medium after being immersed into antibiotics, and a solid culture medium layer is formed on the surface of the cotton swab head. The testing cotton swab is simple in structure and low in cost, a bacterial growth stimulating factor can remarkably stimulate growth of bacteria when the testing cotton swab is used for testing the drug sensitivity of bacteria, the multiplication culture time is remarkably shortened, and accordingly, time of the whole testing process is shortened; due to existence of a bacterial growth indicator, the condition of bacterial colonies formed through growth of bacteria can be indicated by color change, people can distinguish the bacterial colonies with naked eyes even in the early stage of formation of the bacterial colonies, and the testing sensitivity is high.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to a test cotton swab, a preparation method and a test method thereof. Background technique [0002] Antimicrobial susceptibility testing is a test that detects bacterial resistance to antibiotics. The culture medium used in the existing drug susceptibility testing method is divided into two kinds of liquid culture medium and solid culture medium, and its test method is usually the disc test method and the concentration test method, the disc test method uses solid culture medium, and the concentration test method uses liquid medium. The disk test method is to determine the sensitivity of the bacteria to the drug by observing the size of the drug inhibition zone formed by the drug disk when it is pasted on the surface of the solid medium containing specific bacteria; Drugs and different drug concentrations affect bacterial growth to determine the sensitivity of bacteria to ...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12Q1/18
CPCC12Q1/18
Inventor 陈克宏李其昌
Owner GUANGDONG HAID GROUP
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