Testing cotton swab as well as preparation method and testing method thereof
A technology of cotton swabs and swabs, which is applied in the field of medical devices, can solve the problems of non-rapid detection of drug sensitivity, troublesome operation, and high cost, and achieve the effects of shortening the time for proliferation and culture, simple structure, and low production and use costs
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[0038] Another object of the present invention is to provide a method for preparing a test cotton swab, by which a test cotton swab is obtained for testing bacterial drug sensitivity; the preparation method includes the following steps:
[0039] 1) Prepare antibiotic-susceptibility test cotton swabs: formulate antibiotics into a solution, filter with a 0.1-0.5μm filter to prepare an antibiotic solution, and then under aseptic conditions the cotton swab head of the autoclaved cotton swab Insert into the above antibiotic solution;
[0040] 2) Soaking medium: Add double-distilled water to the solid medium, mix it, and then autoclave it. When the medium is cooled to 50-60°C, transfer it to an EP centrifuge tube and transfer the antibiotic-medicine prepared in step 1). The cotton swab head of the sensitive test cotton swab is inserted into the culture medium for 1-3 seconds, taken out and placed in a sterile petri dish to cool, to avoid contact of the cotton swab head with the wall of t...
Example Embodiment
[0049] Example 1
[0050] A test cotton swab, which comprises a cotton swab handle and a cotton swab head. The cotton swab head is immersed in antibiotics and inserted into a solid medium to form a solid medium layer on the surface of the cotton swab; the test cotton swab passes Prepared by the following method:
[0051] 1) Prepare antibiotic-susceptibility test cotton swabs: formulate enrofloxacin into a solution with a concentration of 1-10μg / ml, filter with a 0.1μm filter to prepare an antibiotic solution, and then autoclave it under sterile conditions Insert the cotton swab head of the cotton swab into the above solution;
[0052] 2) Soaking medium: Add double distilled water to the solid medium, mix it, and then autoclave it. When the medium is cooled to 50-60°C, transfer it to an EP centrifuge tube and transfer the antibiotic-medicine prepared in step 1). The cotton swab head of the sensitive test cotton swab is inserted into the culture medium for 1-3 seconds, taken out and ...
Example Embodiment
[0054] Example 2
[0055] A test cotton swab, which comprises a cotton swab handle and a cotton swab head. The cotton swab head is immersed in antibiotics and inserted into a solid medium to form a solid medium layer on the surface of the cotton swab; the test cotton swab passes Prepared by the following method:
[0056] 1) Preparation of antibiotic-susceptibility test cotton swab: florfenicol is prepared into a solution with a concentration of 10-50μg / ml, filtered with a 0.22μm filter to prepare an antibiotic solution, and then autoclaved under aseptic conditions Insert the cotton swab head of the cotton swab into the above solution;
[0057] 2) Soaking medium: Add double-distilled water to the solid medium, mix it, and then autoclave it. When the medium is cooled to 50-60°C, transfer it to an EP centrifuge tube and transfer the antibiotic-medicine prepared in step 1). The cotton swab head of the sensitive test cotton swab is inserted into the culture medium for 1-3 seconds, taken...
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