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Functionalization method for modifying surfaces of magnetic particles with gold nanoparticles

A technology of gold nanoparticles and surface modification, which is applied in the field of preparation of multifunctional nanoparticles, can solve the problems of separation of magnetic particles and gold nanoparticles, variability, and high cost of antigen and antibody, and achieves simple steps, simple operation and low price. Effect

Inactive Publication Date: 2015-04-22
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, antigens and antibodies are more sensitive to the environment and are easily denatured, resulting in the separation of magnetic particles and gold nanoparticles, and the price of antigens and antibodies is expensive

Method used

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  • Functionalization method for modifying surfaces of magnetic particles with gold nanoparticles
  • Functionalization method for modifying surfaces of magnetic particles with gold nanoparticles
  • Functionalization method for modifying surfaces of magnetic particles with gold nanoparticles

Examples

Experimental program
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Effect test

Embodiment 1

[0021] 1) Surface amination of magnetic particles: take 1mL of magnetic particle solution carboxylated on the surface, attract it to the wall of a 1.5mL centrifuge tube with a magnet, remove the liquid with a pipette gun, and add 0.05mol / L carbonate buffer (pH8.2 ), ultrasonic vibration to disperse the magnetic particles, repeat the above cleaning process twice, then add 5% polyetherimide carbonate buffer, and react for 2 hours. The magnetic particles were washed three times with carbonate buffer to remove excess polyetherimide.

[0022] 2) Pegylation on the surface of magnetic particles: add 1 mL of mercapto-polyethylene glycol (molecular weight: 3000)-N-hydroxysuccinimide (HS-PEG-3000-NHS) to the magnetic particles obtained in step 1) and methoxy-polyethylene glycol (molecular weight 2000)-N-hydroxysuccinimide (mPEG-2000-NHS) mixed with carbonate buffer (total PEG concentration of 40mM, HS-PEG-3000-NHS with mPEG-2000-NHS molar ratio is 1:4), reacted for 2h. The magnetic pa...

Embodiment 2

[0025] 1) Surface amination of magnetic particles: take 1mL of magnetic particle solution carboxylated on the surface, attract it to the wall of a 1.5mL centrifuge tube with a magnet, remove the liquid with a pipette gun, and add 0.05mol / L carbonate buffer (pH8.2 ), ultrasonic vibration to disperse the magnetic particles, repeat the above cleaning process twice, then add 5% polyetherimide carbonate buffer solution, and react for 1 h. The magnetic particles were washed three times with carbonate buffer to remove excess polyetherimide.

[0026] 2) Pegylation on the surface of magnetic particles: add 1 mL of mercapto-polyethylene glycol (molecular weight: 3000)-N-hydroxysuccinimide (HS-PEG-3000-NHS) to the magnetic particles obtained in step 1) Mix carbonate buffer with methoxy-polyethylene glycol (molecular weight 2000)-N-hydroxysuccinimide (mPEG-2000-NHS) (total PEG concentration is 20mM, HS-PEG-3000-NHS with mPEG-2000-NHS molar ratio of 1:9), reacted for 2h. The magnetic par...

Embodiment 3

[0029] 1) Surface amination of magnetic particles: take 1mL of magnetic particle solution carboxylated on the surface, attract it to the wall of a 1.5mL centrifuge tube with a magnet, remove the liquid with a pipette gun, and add 0.05mol / L carbonate buffer (pH8.2 ), ultrasonic vibration to disperse the magnetic particles, repeat the above cleaning process twice, then add 5% polyetherimide carbonate buffer, and react for 2 hours. The magnetic particles were washed three times with carbonate buffer to remove excess polyetherimide.

[0030] 2) Pegylation on the surface of magnetic particles: add 1 mL of mercapto-polyethylene glycol (molecular weight: 3000)-N-hydroxysuccinimide (HS-PEG-3000-NHS) to the magnetic particles obtained in step 1) and methoxy-polyethylene glycol (molecular weight 2000)-N-hydroxysuccinimide (mPEG-2000-NHS) mixed with carbonate buffer (total PEG concentration of 10mM, HS-PEG-3000-NHS with mPEG-2000-NHS molar ratio is 1:4), reacted for 2h. The magnetic pa...

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Abstract

The invention provides a simple functionalization method for modifying surfaces of magnetic particles with gold nanoparticles, namely a surface carboxylation method for modifying the surfaces of the magnetic particles with the gold nanoparticles in a functionalization mode. Firstly, polyetherimide (PEI) is used for conducting amination on carboxyl functional groups on the surfaces of the magnetic nanometer or micrometer particles; then, polyethylene glycol (PEG) macromolecules are coupled to the aminated magnetic particles, wherein sulfhydryl (SH) radical groups are located at one end of each PEG macromolecule, and N-Hydroxysuccinimide is located at the other end of each PEG macromolecule; finally, the magnetic particles react with the gold nanoparticles, and therefore the magnetic nanometer or micrometer particles are modified with the gold nanoparticles. According to the method, the steps are simple, operation is simple, price is relatively low, and a nanometer-level multifunctional research platform can be built.

Description

technical field [0001] The invention relates to a method for preparing multifunctional nanoparticles, in particular to a method for functionalizing the surface of magnetic particles to modify gold nanoparticles for biomolecular labeling, separation and purification, clinical molecular diagnosis, magnetic targeting and the like. Background technique [0002] Due to their unique optical properties and good chemical stability, gold nanoparticles have broad application prospects in nanoelectronics, nanophotonics, sensors, biomolecular labeling, and photothermal therapy. [0003] Magnetic micro- and nanoparticles can be used in biomolecular separation, magnetically targeted drugs, medical detection and diagnosis due to their magnetic induction properties in a magnetic field. [0004] Linking magnetic particles and gold nanoparticles can establish a multifunctional research platform at the nanometer level, which is expected to be used in biological imaging and diagnosis. For exam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B82B3/00B82Y30/00
Inventor 何丹农钟建姜杰颜娟王萍
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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