Method for preparing urokinase and freeze-dried powder of urokinase

A technology of urokinase and freeze-dried powder, which is applied in the preparation method of urokinase and the field of freeze-dried powder, can solve the problems of high production cost, inability to ensure high purity and high activity yield of large molecular weight urokinase, and reduce production cost, reduce side effects, and ensure curative effect
CN110894495AActive Publication Date: 2020-03-20JIANGSU YOULIKA BIOTECHNOLOYG CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGSU YOULIKA BIOTECHNOLOYG CO LTD
Publication Date
2020-03-20

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Abstract

The invention relates to a method for preparing high-purity macromolecular urokinase and freeze-dried powder of the urokinase and belongs to the technical field of biochemical engineering. The methodspecifically comprises the steps: (1) preparing a urokinase crude product; (2) preparing a urokinase fine product; and (3) carrying out purifying, wherein in the step (3), the urokinase fine product obtained in the step (2) is dissolved, and then, the urokinase fine product solution is added into a cation exchange chromatography column for purification; and the purified urokinase is subjected to freeze-drying, thereby obtaining the freeze-dried powder of the urokinase. Fillers filled in the cation exchange chromatography column comprise one of Capto S impAct, SP sepharose HP and source 30S. According to the method for preparing the urokinase and the freeze-dried powder of the urokinase, high-resolution ion-exchange chromatography is selected, so that on the premise of guaranteeing purity,activity and yield, the production cost is reduced greatly, and large-scale production of high-purity high-molecular-weight urokinase becomes possible.
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Description

technical field

[0001] The invention belongs to the technical field of biochemical engineering, and in particular relates to a preparation method of urokinase and freeze-dried powder thereof. Background technique

[0002] Urokinase is a thrombolytic drug extracted from fresh human urine. It can activate plasminogen and convert it into active plasmin. dissolve. Therefore, it is mostly used clinically to treat thrombosis, thromboembolism and other diseases. When urokinase is used in combination with anticancer agents, because it can dissolve fibrin around cancer cells, anticancer agents can penetrate into cancer cells more effectively, thereby improving the ability of anticancer agents to kill cancer cells. So urokinase is also a good adjuvant therapy for cancer, and it has no antigenicity problem and can be used for a long time.

[0003] Urokinase is a serine protease produced by human renal tubular epithelial cells. It is a basic protein with an isoelectric point of aroun...

Claims

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