The invention provides a biological enzyme purification separating device. The biological enzyme purification separating device comprises a raw material storage tank, a Graef pump, a micro-filter tube, a rejection liquid collection pump, a wash tank, a flow adjusting device, a valve, a pressure gage, a flow gauge, and a material tube. The biological enzyme purification separating device can achieve continuous filtering and separating of high turbidity liquid under the fully sealed condition and maintaining of high activity of zymin. Due to the fact that the micro-filter tube, with the pore diameter of 0.1-0.2 microns and high-strength, acid-base resistance and corrosion resistance, of a hollow fiber organic membrane or ceramic or acstainless steel component is adopted, the biological enzyme purification separating device can efficiently conduct edulcoration and degerming and achieve separation and purification effect, at the same time, cannot damage an original ingredient, an original structure, an original special flavor, and original energy activity, improves sanitary quality of products. The biological enzyme purification separating device is widely applied for filtering and separation of variousbiological enzymes with strict demands for microorganismsanitation and enzyme activity and at the same time is further suitable for filtering and separation of certain simple liquid state drinks and condiments.