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Codonopsis pilosula uniform polysaccharide CPPib, preparation and application thereof

Codonopsis polysaccharide, homogeneous technology, applied in Codonopsis polysaccharide CPP1b and its preparation and application field, can solve problems such as unreported research

Active Publication Date: 2013-06-12
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, there is no report on the research on the homogeneous polysaccharide CPP1b of Codonopsis pilosula

Method used

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  • Codonopsis pilosula uniform polysaccharide CPPib, preparation and application thereof
  • Codonopsis pilosula uniform polysaccharide CPPib, preparation and application thereof
  • Codonopsis pilosula uniform polysaccharide CPPib, preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0121] The preparation method of Codonopsis pilosula homogeneous polysaccharide CPP1b comprises the following steps:

[0122] (1) Preparation of Codonopsis Codonopsis Crude Polysaccharide

[0123] Take Codonopsis pilosula (collected in Wenxian County, Gansu Province), pulverize, reflux with 95% ethanol to degrease, dry the dregs, weigh them, extract 4 times with 9 times the amount of water at 94°C, each time for 2 hours, filter, and combine the filtrates. Concentrate under reduced pressure after centrifugation, add 95% ethanol with stirring to make the ethanol concentration reach 30%, let stand overnight at 4-8°C, centrifuge (4000 rpm, 10 min), collect the precipitate, and freeze-dry. Use Savag method to remove protein and hydrogen peroxide method to depigment, concentrate to a certain volume, add ethanol to make the final concentration reach 80%, remove supernatant, precipitate and freeze-dry to obtain Codonopsis pilosula crude polysaccharide CPP1.

[0124] (2) Separation a...

Embodiment 2

[0127] The preparation method of Codonopsis pilosula homogeneous polysaccharide CPP1b comprises the following steps:

[0128] (1) Preparation of Codonopsis Codonopsis Crude Polysaccharide

[0129] Take Codonopsis pilosula (collected in Wenxian County, Gansu), crush it, reflux it with 95% ethanol to degrease, dry the dregs, weigh them, extract 4 times with 20 times the amount of water at 90°C, 1 hour each time, filter, combine the filtrates, and centrifuge Afterwards, concentrate under reduced pressure, add 95% ethanol with stirring to make the ethanol concentration reach 20%, let stand overnight at 4-8°C, centrifuge (3000 rpm, 15min), collect the precipitate, and freeze-dry. Use Savag method to remove protein and hydrogen peroxide method to depigment, concentrate to a certain volume, add ethanol to make the final concentration reach 80%, remove supernatant, precipitate and freeze-dry to obtain Codonopsis pilosula crude polysaccharide CPP1.

[0130] (2) Separation and purific...

Embodiment 3

[0133] The preparation method of Codonopsis pilosula homogeneous polysaccharide CPP1b comprises the following steps:

[0134] (1) Preparation of Codonopsis Codonopsis Crude Polysaccharide

[0135] Take Codonopsis pilosula (collected in Wenxian County, Gansu Province), crush it, reflux with 95% ethanol to degrease, dry the dregs, weigh them, extract 3 times with 10 times the amount of water at 90°C for 2 hours each time, filter, combine the filtrates, and centrifuge Concentrate under reduced pressure, add 95% ethanol with stirring to make the ethanol concentration reach 35%, let stand overnight at 4-8°C, centrifuge (4000 rpm, 10min), collect the precipitate, and freeze-dry. Use Savag method to remove protein and hydrogen peroxide method to depigment, concentrate to a certain volume, add ethanol to make the final concentration reach 80%, remove supernatant, precipitate and freeze-dry to obtain Codonopsis pilosula crude polysaccharide CPP1.

[0136] (2) Separation and purificati...

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Abstract

The invention discloses codonopsis pilosula uniform polysaccharide CPPib which is mainly composed of, by Mole percentage, 44.28% of rhamnose, 20.42% of galactose, 11.32% of arabinose and 23.97% of galacturonic acid, weight-average molecular weight of the codonopsis pilosula uniform polysaccharide CPPib is 1.45*105Da, and main carbohydrate chains are 1,2 connected rhamnose (rha, 45%), 1,4-connected galacturonic acid (GalA, 25%) and 1,2,6 connected galactose (Gal, 30%). A preparation method of the codonopsis pilosula uniform polysaccharide CPPib has the advantages of being simple, scientific, reasonable, and capable of preparing products with very high purity with only need of passing through an anion exchanging column chromatograph once. The prepared products have the advantages of having anti-tumor effect, educational effect, very high clinical application value and healthcare function.

Description

technical field [0001] The invention relates to a plant extract, in particular to CPP1b, a homogeneous polysaccharide from Codonopsis pilosula, and its preparation and application. Background technique [0002] Codonopsis ( Codonopsis pilosula ) is the dried root of Codonopsis genus in the Platycodonaceae family. It is a commonly used traditional Chinese medicine in China and is often used as a substitute for the precious medicinal material ginseng. Traditional Chinese medicine believes that it has the effects of nourishing the middle and replenishing qi, harmonizing the spleen and stomach, and eliminating polydipsia. Codonopsis polysaccharide is a main component of Codonopsis pilosula. Yongxu Sun et al. (Yongxu Sun, Jicheng Liu. Structural characterization of a water-soluble polysaccharide from the Roots of Codonopsis pilosula and its immunity activity [J]. International Journal of Biological Macromolecules, 2008, 43: 279-282.) isolated a neutral Codonopsis polysacchari...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P35/00A61P25/00
Inventor 胡芳弟
Owner LANZHOU UNIVERSITY
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