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Ziyang selenium-rich green tea selenium-containing polysaccharide, and preparation method and application thereof

A technology for selenium-enriched green tea and selenium polysaccharide, which is applied in the directions of food preparation, medical preparations containing active ingredients, and applications, can solve the problems of low functional factors of natural selenium-enriched tea and no reports of natural selenium-enriched tea polysaccharides, and achieves obvious results. Immunomodulatory effect, liver damage protection, effect of simple method

Active Publication Date: 2012-04-18
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few in-depth research reports on the functional factors of natural selenium-enriched tea, especially the natural selenium-enriched tea polysaccharides are hardly reported.

Method used

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  • Ziyang selenium-rich green tea selenium-containing polysaccharide, and preparation method and application thereof
  • Ziyang selenium-rich green tea selenium-containing polysaccharide, and preparation method and application thereof
  • Ziyang selenium-rich green tea selenium-containing polysaccharide, and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The selenium-enriched green tea of ​​Ziyang of this embodiment contains selenium polysaccharide. The composition of the selenium-containing polysaccharide is calculated by mass percentage: selenium 8.5 ppm, and the balance is heteropolysaccharide; the composition of the heteropolysaccharide is calculated as mannose by mole percentage 5.3%, ribose 2%, rhamnose 5%, glucuronic acid 3.5%, galacturonic acid 3.8%, glucose 30%, xylose 5.2%, galactose 23.2%, arabinose 22%.

[0042] The preparation method includes the following steps:

[0043] (1) Dry Ziyang selenium-enriched green tea leaves under natural conditions, and then crush them into tea powder;

[0044] (2) Add distilled water 10 times the mass of the tea powder and at a temperature of 80° C. to the tea powder described in step (1), extract for 2 hours and then filter;

[0045] (3) Repeat step (2) twice, combine the filtrate and concentrate to 1 / 4 of the volume of the filtrate;

[0046] (4) Add 5 times the volume of absolute et...

Embodiment 2

[0050] The selenium-enriched green tea of ​​Ziyang of this embodiment contains selenium polysaccharide. The composition of the selenium-containing polysaccharide is calculated by mass percentage: 1.5 ppm of selenium, and the balance is heteropolysaccharide; the composition of the heteropolysaccharide is calculated as mannose by mole percentage 5.3%, ribose 1%, rhamnose 3.5%, glucuronic acid 2%, galacturonic acid 2.6%, glucose 34.2%, xylose 3.6%, galactose 22.2%, arabinose 25.6%.

[0051] The preparation method includes the following steps:

[0052] (1) Dry Ziyang selenium-enriched green tea leaves under natural conditions, and then crush them into tea powder;

[0053] (2) Add distilled water 15 times the mass of the tea powder and at a temperature of 80° C. to the tea powder described in step (1), extract for 2 hours and then filter;

[0054] (3) Repeat step (2) twice, combine the filtrate and concentrate to 1 / 4 of the volume of the filtrate;

[0055] (4) Add 3 times the volume of abso...

Embodiment 3

[0059] The selenium-enriched green tea of ​​Ziyang of this embodiment contains selenium polysaccharide. The composition of the selenium-containing polysaccharide is calculated by mass percentage: 5 ppm of selenium, and the balance is heteropolysaccharide; the composition of the heteropolysaccharide is calculated by mole percentage: mannose 4.9 %, ribose 1.2%, rhamnose 4%, glucuronic acid 2.9%, galacturonic acid 3.4%, glucose 33.5%, xylose 5%, galactose 21.8%, arabinose 23.3%.

[0060] The preparation method includes the following steps:

[0061] (1) Dry Ziyang selenium-enriched green tea leaves, and then crush them into tea powder;

[0062] (2) Add 12 times the mass of the tea powder to distilled water at a temperature of 80°C to the tea powder described in step (1), extract for 2 hours and then filter;

[0063] (3) Repeat step (2) 3 times, combine the filtrate and concentrate to 1 / 4 of the volume of the filtrate;

[0064] (4) Add 4 times the volume of absolute ethanol to the concentra...

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Abstract

The invention discloses a Ziyang selenium-rich green tea selenium-containing polysaccharide, which comprises the following components in percentage by mass: 1.5-8.5 ppm selenium and the balance of heterosaccharide. The heterosaccharide comprises the following components in percentage by mol: 3.3-5.3% of mannitose, 1-2% of ribose, 3.5-5% of rhamnose, 2-3.5% of glucuronic acid, 2.6-3.8% of galacturonic acid, 30-34.2% of glucose, 3.6-5.6% of xylose, 19.8-23.2% of galactose and 21.5-25.6% of arabinose. The invention also provides a preparation method and application of the Ziyang selenium-rich green tea selenium-containing polysaccharide. The preparation method is simple and convenient, has the advantage of low cost, and is suitable for popularization; and the selenium-containing polysaccharide has the action of enhancing immunity, has obvious inhibiting actions on growth of colon cancer cells, and has obvious protective actions on hepatic injury.

Description

Technical field [0001] The invention belongs to the technical field of tea products and biological engineering, and specifically relates to a selenium polysaccharide of Ziyang selenium-enriched green tea, and a preparation method and application thereof. Background technique [0002] Tea leaves can be divided into three categories: green tea (unfermented tea), oolong tea (semi-fermented tea) and black tea (fermented tea). Among them, green tea has the characteristics of high fragrance, mellow taste, beautiful shape, and resistance to brewing. It is the most produced type of tea in my country. Its variety of patterns and colors ranks first in the world. It exports tens of thousands of tons per year, accounting for the world tea market trade volume. About 70% of the total. Modern scientific research shows that green tea plays an important role in the pretreatment of many diseases, especially in the prevention and treatment of chronic diseases such as cardiovascular disease and tumo...

Claims

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Application Information

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IPC IPC(8): C08B37/00A23L1/09A61K31/715A61P37/04A61P35/00A61P1/16
Inventor 杨兴斌赵燕何念武王东英田灵敏
Owner SHAANXI NORMAL UNIV
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