Antibacterial peptide LZ1 and application of antibacterial peptide in preparation of antibacterial medicament

An antibacterial peptide and antibacterial drug technology, applied in the field of antibacterial drug preparation, can solve the problems of low antibacterial activity, cytotoxicity of host cells, etc., and achieve the effects of strong bactericidal effect, extremely low eukaryotic cytotoxicity, and convenient artificial synthesis

Inactive Publication Date: 2013-02-13
SUZHOU KANGER BIOLOGICAL MEDICAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Antimicrobial peptides from natural sources have immunogenicity due to their large molecular weight, low antibacterial activity, cytotoxicity to host cells, or hemolysis, which limits the promotion and application of antimicrobial peptides as antibacterial drugs. Smaller, stronger antibacterial activity, especially antimicrobial peptides without hemolysis or cytotoxicity are the most critical factors to solve the problem of antimicrobial peptides as antibacterial drugs

Method used

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  • Antibacterial peptide LZ1 and application of antibacterial peptide in preparation of antibacterial medicament
  • Antibacterial peptide LZ1 and application of antibacterial peptide in preparation of antibacterial medicament
  • Antibacterial peptide LZ1 and application of antibacterial peptide in preparation of antibacterial medicament

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: the preparation of antimicrobial peptide LZ1

[0021] 1. Chemical synthesis method of antimicrobial peptide LZ1: According to the amino acid sequence described in the summary of the invention, its entire sequence was synthesized with an automatic peptide synthesizer (433A, Applied Biosystems), and purified by HPLC reverse-phase column chromatography desalting.

[0022] Ⅱ. Molecular weight was determined by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF).

[0023] Ⅲ. The purity of the purified antibacterial peptide LZ1 was identified by high performance liquid chromatography (HPLC), the molecular weight was determined by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF), the isoelectric point was determined by isoelectric focusing electrophoresis, and an automatic amino acid sequencer was used to determine Amino acid sequence structure.

[0024] The antibacterial peptide LZ1 co...

Embodiment 2

[0025] Embodiment 2: antibacterial experiment of antimicrobial peptide LZ1:

[0026] Minimum inhibitory concentration (minimal inhibitory concentration, MIC): is the lowest sample concentration where no bacterial growth can be detected. Using the two-fold dilution method, such as figure 1 , the specific method is as follows:

[0027] Bacteria were inoculated on Luria-Bertani (LB) solid medium and cultured upside down in a 37°C incubator. After the colony grows, pick a single colony with an inoculation loop and transfer it to LB liquid medium, and shake it in a 37°C incubator until the logarithmic growth phase. Detect the OD600 of the bacterial solution on the ultraviolet spectrophotometer, according to 1OD600=1×10 9 CFU / ml Dilute the bacterial solution to 2×10 with liquid LB medium 5 CFU / ml. Add 100 μl of LB liquid medium to each well of a sterile 96-well plate, then add 100 μl of antimicrobial peptide samples diluted to a certain concentration to the first well and filte...

Embodiment 3

[0032] Example 3: Hemolytic Activity Experiment of Antimicrobial Peptide LZ1:

[0033] Rabbit heart blood collection or human vein blood collection, the collected blood and Alsever Solution (Alsever Solution, 8.0g sodium citrate, 0.55g citric acid, 20.5g glucose, 4.2g NaCl, add deionized water to 1L, adjust the pH to 6.1 , stored at 4°C after autoclaving) were mixed at a ratio of 1:1 and placed in a centrifuge tube, centrifuged at 1000 rpm for 5 minutes, and washed with normal saline until the supernatant was no longer red. Dilute the washed red blood cells with normal saline to 10 7 -10 8 concentrated suspension. The above-mentioned diluted erythrocyte suspension was incubated with samples of different concentrations dissolved in normal saline at 37°C for 30 minutes, then centrifuged at 1000 rpm for 5 minutes, and the supernatant was measured for absorbance at 540 nm. Normal saline was used as the negative control, and Triton X-100 was used as the positive control. The he...

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Abstract

An antibacterial peptide LZ1 is an artificially designed and synthesized active polypeptide and contains 15 amino acid residues, the molecular weight is 2,228.77Da, and the isoelectric point is 12.05; and the full sequence of the antibacterial peptide is valine-lysine-arginine-tryptophan-lysine-lysine-tryptophan-tryptophan-arginine-lysine-tryptophan-lysine-lysine-tryptophan-valine-NH2. The antibacterial peptide LZ1 is small in molecular weight, strong in bactericidal effect and wide in antibacterial spectrum, almost does not have hemolytic activity or eukaryocyte toxicity, is a small molecular antibacterial peptide with application value, is convenient to artificially synthesize, and can be used for preparing an antibacterial medicament.

Description

Technical field: [0001] The invention belongs to the technical field of polypeptide drugs in biochemistry, and in particular relates to antibacterial peptide LZ1 and the use of the antibacterial peptide in preparing antibacterial drugs. Background technique: [0002] Since the discovery of antibiotics such as penicillin, the treatment of bacterial infectious diseases has been fundamentally improved. The use of antibiotics has saved the lives of countless patients and extended the average life span of humans. However, with the large-scale use or abuse of antibiotics, especially in developing countries, it has led to the emergence and spread of some drug-resistant bacteria, including some highly virulent pathogenic bacteria, such as Staphylococcus and Streptococcus pneumoniae . Therefore, finding safe and effective antibacterial drugs that are not prone to drug resistance has become the direction of competition and efforts of scientists all over the world. [0003] Antimicro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K38/10A61P31/04
Inventor 赖仞张治业
Owner SUZHOU KANGER BIOLOGICAL MEDICAL
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